硝化抑制剂对农业废物好氧堆肥理化性质及反硝化功能基因的影响

发布时间：2018-02-28 13:36

本文关键词： 好氧堆肥硝化抑制剂理化性质反硝化功能基因　出处：《环境工程学报》2016年08期 　论文类型：期刊论文

【摘要】：以秸秆等农业废物为主要原料,添加硝化抑制剂双氰胺(dicyandiamide,DCD)进行静态好氧堆肥实验,设置相同堆肥条件不添加DCD作为空白对照,分析了堆肥过程中各个时期不同处理的p H值、NH+4-N浓度、NO-3-N浓度和水溶性有机碳(WSC)理化参数的变化趋势,并应用定量聚合酶链式反应(real-time PCR)技术对参与反硝化过程的功能基因(nir K、nir S和nos Z)丰度随时间的变化情况进行了研究。结果表明,添加DCD明显地改变了堆肥过程的氮素转化,两组堆肥样品的p H值、NH+4-N浓度、NO-3-N浓度有显著差异(p0.001),但是对WSC的下降过程没有明显影响。DCD对nir K基因的抑制效果最显著,同时nir S和nos Z基因因为理化参数的改变而受到了间接影响。
[Abstract]:The static aerobic composting experiment was carried out by adding nitrification inhibitor dicyandiamide DCD with straw and other agricultural wastes as main raw materials. The same composting conditions were not added with DCD as the blank control. The changing trend of pH value NH 4-N concentration and water soluble organic carbon (WSCC) physical and chemical parameters of different treatments during composting process were analyzed. Quantitative polymerase chain reaction (PCR) real-time PCR technique was used to study the changes of the abundance of functional genes (nir Knir S and nos Z) involved in the denitrification process. The results showed that the addition of DCD significantly changed the nitrogen conversion in the composting process. There was significant difference in pH value of NH _ 4-N and no _ 3-N concentration between the two groups, but there was no significant effect on the decrease of WSC. DCD had the most significant inhibitory effect on nir K gene. At the same time, nir S and nos Z genes were indirectly affected by the change of physical and chemical parameters.
【作者单位】：湖南大学环境科学与工程学院;环境生物与控制教育部重点实验室(湖南大学);湖南城市学院化学与环境工程学院;湖南农业大学资源环境学院;
【基金】：国家自然科学基金资助项目(51378190;21276069) 湖南省自然科学基金资助项目(13JJB002) 中央高校基本科研业务费专项资金资助
【分类号】：X71;S141.4

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