新型拟除虫菊酯降解酶的分子改造及降解特性

发布时间：2018-03-09 17:18

  本文选题：拟除虫菊酯　切入点：农药降解酶　出处：《中山大学学报(自然科学版)》2017年04期 　论文类型：期刊论文

【摘要】：为了获得稳定性和酶活性提高的拟除虫菊酯类降解酶,利用易错PCR技术对来源于海底泥宏基因组文库的新型酯酶基因(est825)进行体外定向进化,获得了一个酶活性和热稳定性均提高的突变酶(Est M46)。与野生型相比其酶活力提高1.5倍;最适反应温度提高了5℃,同时热稳定性明显增强。此外,Est M46对氯氟氰菊酯、氯氰菊酯、氰戊菊酯和溴氰菊酯的降解率分别提升至92.21%、99.75%、93.21%和89.48%。这对降低酶的生产成本、促进工业化发展和环境保护有重要意义。
[Abstract]:In order to obtain pyrethroid ester-degrading enzymes with improved stability and enzyme activity, a novel esterase gene, est825, which was derived from the submarine mud macro genomic library, was developed by error-prone PCR in vitro. A mutant enzyme, est M46, was obtained. The enzyme activity was 1.5 times higher than that of wild type, the optimum reaction temperature was increased 5 鈩，

本文编号：1589499