高效纤维素优势分解菌的筛选和鉴定

发布时间：2018-03-09 18:07

本文选题：纤维素分解菌　切入点：酶活力测定　出处：《中国土壤与肥料》2017年02期 　论文类型：期刊论文

【摘要】：本试验基于获得高效纤维素优势分解菌的目的,通过分离纯化初步得到30株菌株,利用刚果红染色法初筛共得到14株纤维素分解菌,并通过滤纸条崩解实验进一步进行筛选得到5株效果较好的纤维素分解菌,通过发酵产酶利用DNS显色法测定CMC酶活力和FPA酶活力最终确定了4株优势纤维素分解菌,通过测定4株菌株的羧甲基纤维素酶(CMCase)、滤纸酶(FPA)以及β-葡萄糖苷酶(β-Gase)活,验证4株纤维素优势分解菌的产酶能力,并分别命名为X-1、X-6、X-7和X-11,并将该4株优势纤维素分解菌应用于秸秆的液态发酵,其对秸秆的降解率较自然降解相比,降解率分别提高了31.92%、40.15%、35.29%和39.98%。对4株优势菌株进行了分子鉴定,根据16S r DNA序列比对结果表明,菌株X-1、X-7和X-11均为粪产碱杆菌;菌株X-6属于解糖假苍白杆菌。
[Abstract]:The advantage of efficient cellulose decomposing bacteria based on the purpose of the 30 strains obtained by the separation and purification by Congo red staining method for screening a total of 14 strains of cellulose decomposing bacteria, and the experiment further screened 5 strains of good effect of fiber cellulose decomposing bacteria through the collapse of filter, through fermentation using DNS color determination of CMC and FPA enzyme activity to 4 strains of dominant cellulose decomposing bacteria were determined, through the determination of 4 strains of carboxymethyl cellulose (CMCase), filter paper enzyme (FPA) and beta glucosidase (beta -Gase), enzyme production capacity verification of 4 strains of cellulose decomposing bacteria were dominant. Named X-1, X-6, X-7 and X-11, and the advantages of liquid fermentation of 4 strains of cellulose decomposing bacteria used in straw, the straw degradation rate was 31.92%, compared to natural degradation, the degradation rate increased by 40.15%, 35.29% and 39.98%. of 4 strains of dominant The strain was identified. According to the 16S R DNA sequence alignment, the strain X-1, X-7 and X-11 were all Bacillus faecalis, and the strain X-6 belonged to Bacillus Pseudomonas.

【作者单位】：东北农业大学资源与环境学院;
【分类号】：S141.4

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