不同施氮水平下再生水灌溉对土壤细菌群落结构影响研究

发布时间：2018-04-09 18:57

本文选题：再生水　切入点：细菌群落多样性　出处：《环境科学学报》2017年01期

【摘要】：微生物是土壤环境变化的敏感因子,为探明再生水灌溉和氮素减量施用对土壤环境的影响,以温室棚栽番茄为研究对象,借助Miseq高通量技术,比较研究了再生水灌溉下氮素常规施肥和氮肥减量施用对土壤细菌群落结构所产生的影响,并采用冗余分析(Redundancy Analysis,RDA)方法分析导致土壤细菌群落结构差异的因素.结果表明:再生水灌溉对土壤硝化螺菌门(Nitrospirae)、芽单胞菌门(Gemmatimonadetes)、厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)和放线菌门(Actinobacteria)群落结构的影响明显;再生水灌溉土壤细菌共53个属,其中41个属是再生水灌溉和清水灌溉土壤的共有菌属,其余12个属是再生水灌溉土壤的特有菌属.随氮素施用水平的降低,土壤细菌种群优势度呈先增加后降低然后再增加的趋势,减少氮肥施用有利于土壤细菌种群丰富度和多样性的增加.土壤微生物群落结构受土壤化学特性的影响.再生水灌溉能够促进与土壤碳、氮转化相关的微生物的增长,改变土壤微生物的群落结构.
[Abstract]:Microorganism is a sensitive factor to explore the changes of soil environment, reclaimed water irrigation and nitrogen reduction effects on soil environment in greenhouse, greenhouse tomato as the research object, using Miseq high-throughput technologies, a comparative study of the effects of reclaimed water irrigation under conventional fertilization of nitrogen and nitrogen reduction application of soil bacterial community structure, and by using redundancy analysis (Redundancy Analysis RDA) method to analyze the factors that lead to the difference of the soil bacterial community structure. The results showed that: the soil nitrification magneticum door of reclaimed water irrigation (Nitrospirae), gemmatimonadetes (Gemmatimonadetes), Firmicutes, Proteobacteria (Firmicutes) (Proteobacteria) and actinobacteria (Actinobacteria) significantly affect the community structure; reclaimed water irrigation soil bacteria belonging to 53 genera, including 41 genera is irrigated with reclaimed water and fresh water irrigation soil total bacteria, the remaining 12 is recycled water The unique irrigation soil bacteria genera. With the decrease of nitrogen application, soil bacterial population dominance was increased and then decreased after the first increase, reduction of nitrogen fertilizer increased soil bacterial species richness and diversity. The influence of soil microbial community structure was affected by soil chemical characteristics. Reclaimed water irrigation can promote soil carbon and the conversion of nitrogen, microbial growth, soil microbial community structure change.

【作者单位】：中国农业科学院农田灌溉研究所;中国农业科学院研究生院;中国农业科学院农业水资源高效安全利用重点开放实验室;
【基金】：国家高技术研究发展(863)计划项目(No.2012AA101404) 国家自然科学基金(No.51479201)~~
【分类号】：S154.3;S273

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