香蕉不同生育期根际土壤细菌群落变化研究

发布时间：2018-04-16 19:03

本文选题：香蕉 + 根际　；参考：《热带作物学报》2016年06期

【摘要】：土壤微生物是土壤质量和健康的敏感性指标。研究认为,根际微生物区系失调是作物土传病害发生的一个重要原因。通过对香蕉不同生长期根际土壤中细菌多样性和数量的研究,对香蕉枯萎病发生的机理及生物防治具有重要意义。实验采用Real-time PCR和T-RFLP技术,对巴西蕉不同生长期根际细菌数量和多样性进行了研究。结果表明:随着香蕉生长期的增加,根际土壤细菌数量从3月的1.1×1010copies/g土壤逐渐增加到7月的2.7×1010copies/g土壤,随后逐渐减少。然而,根际细菌多样性从3月到7月呈逐渐减少趋势,到7月达到最低,为1.99。另外,不同月份中优势菌的种类和在总细菌中所占的比例也不同。在实验期内,随着香蕉生育期的延长,某些细菌种类大量繁殖,土壤根际细菌群落结构失衡,土壤微生物环境有恶化趋势。
[Abstract]:Soil microorganisms are sensitive indicators of soil quality and health.It is considered that the imbalance of rhizosphere microflora is an important cause of soil-borne diseases of crops.The study on the diversity and quantity of bacteria in rhizosphere soil of banana at different growth stages is of great significance to the occurrence mechanism and biological control of banana wilt.Real-time PCR and T-RFLP techniques were used to study the number and diversity of rhizosphere bacteria in different growth stages of Canna brasiliensis.The results showed that the number of bacteria in rhizosphere soil increased from 1. 1 脳 1010copies/g in March to 2. 7 脳 1010copies/g in July, and then decreased with the increase of banana growth period.However, the rhizosphere bacterial diversity decreased gradually from March to July and reached its lowest level of 1.99 in July.In addition, the dominant bacteria in different months and the percentage of the total bacteria are also different.During the experiment period, with the extension of banana growth period, some bacterial species propagated in large numbers, the soil rhizosphere bacterial community structure was out of balance, and the soil microbial environment had a tendency to deteriorate.
【作者单位】：农业部热带作物生物学与遗传资源利用重点实验室中国热带农业科学院热带生物技术研究所;
【基金】：海南省重大科技项目子课题热带生物种质与基因资源研究(No.ZDZX2013023-1)
【分类号】：S154.3;S668.1

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