长期施肥对大麦-双季稻种植方式中大麦根际土壤微生物群落功能多样性的影响

发布时间：2018-04-18 07:44

本文选题：不同施肥 + 双季稻田复种　；参考：《四川农业大学学报》2017年02期

【摘要】：【目的】微生物群落功能多样性是土壤质量变化的敏感指标,施肥措施与土壤微生物群落功能多样性关系密切。本文的目的在于探明不同施肥措施对土壤微生物群落功能多样性的影响。【方法】以双季稻主产区肥料长期定位试验田为研究对象,应用Biolog技术分析了化肥(MF)、秸秆还田+化肥(RF)、30%有机肥+70%化肥(LOM)、60%有机肥+40%化肥(HOM)、无肥对照(CK)不同施肥处理对大麦成熟期双季稻田根际土壤微生物功能多样性的影响,从根际土壤微生物功能多样性的角度评价施肥对土壤质量的影响。【结果】MF、LOM和HOM处理大麦根际土壤微生物对碳源的利用程度均显著高于其他处理,而RF处理大麦根际土壤微生物对碳源的利用程度低于CK处理。在大麦成熟期,Richness和Shannon指数大小顺序均表现为:HOMLOMRFCKMF,Mc Intosh指数均以HOM和LOM处理为最高,其次为RF和CK处理,MF处理最低。土壤微生物碳源利用的主成分分析结果表明,各施肥处理大麦根际土壤微生物群落利用的碳源主要为氨基酸类和糖类,不同施肥处理间碳源利用类型存在明显的差异。【结论】不同施肥处理对大麦根际土壤微生物功能多样性产生了不同影响,长期有机无机肥配施有利于维持稻田根际土壤微生物群落多样性。
[Abstract]:[objective] functional diversity of microbial communities is a sensitive indicator of soil quality change, and fertilization measures are closely related to functional diversity of soil microbial communities.The purpose of this paper is to investigate the effects of different fertilization measures on the functional diversity of soil microbial communities.Biolog technique was used to analyze the effects of different fertilization treatments on the microbial functional diversity of rhizosphere soil in the double cropping rice field at the mature stage of barley. The effects of different fertilization treatments on the microbial functional diversity in the rhizosphere soil of the double cropping rice field at the mature stage of barley were analyzed, including 30% organic fertilizer, 70% organic fertilizer, 60% organic fertilizer, 40% organic fertilizer, 40% chemical fertilizer, and 40% CK, respectively.The effects of fertilization on soil quality were evaluated from the point of view of microbial functional diversity in rhizosphere soil. [results] the utilization of carbon sources in barley rhizosphere soil treated with MFLOM and HOM was significantly higher than that of other treatments.However, the utilization of carbon in rhizosphere soil of barley treated with RF was lower than that of CK.At the mature stage of barley, the order of Shannon index and Richness index showed that the Intosh index of HOM and LOM were the highest, and that of RF and CK treatments were the lowest.The results of principal component analysis of soil microbial carbon source utilization showed that the main carbon sources of soil microbial community utilization of barley rhizosphere were amino acids and sugars.[conclusion] different fertilization treatments have different effects on microbial functional diversity in barley rhizosphere soil.Long-term application of organic and inorganic fertilizers was beneficial to maintain the diversity of microbial communities in the rhizosphere of paddy fields.
【作者单位】：湖南生物机电职业技术学院;湖南省土壤肥料研究所;湖南省宁乡县农业局;
【基金】：国家自然科学基金“双季稻田根际土壤微生物群落结构及其多样性对长期施肥措施的响应机制”(31571591);“不同施肥模式对大麦-双季稻田甲烷氧化菌多样性及活性影响的研究”(31201178)
【分类号】：S154.36

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