安徽查湾自然保护区常绿阔叶林不同林分土壤微生物群落代谢特征

发布时间：2018-05-07 14:55

本文选题：土壤微生物群落 + 林分因子　；参考：《生态环境学报》2017年07期

【摘要】：为探讨亚热带常绿阔叶林不同林分土壤微生物群落特征,在安徽祁门县查湾自然保护区亚热带常绿阔叶林内,分别选择以甜槠(Castanopsis eyrei(Champ.)Tutch.)为单一优势种的林分P1,以青冈栎(Cyclobalanopsis glauca(Thunb.)Oerst.)、甜槠和马尾松(Pinus massoniana Lamb.)为优势种的林分P2,各设置3个重复样地,采用Biolog方法探讨了土壤微生物群落功能多样性与林分结构的关系。结果表明,林分P2土壤养分(铵态氮、硝态氮、可溶性有机碳氮)均高于林分P1,但没有显著差异(P0.05);两种林分0~10 cm土层的土壤养分含量明显高于10~20 cm土层。培养48 h后,林分P2的0~10 cm与10~20 cm土层AWCD均大于P1,并且0~10 cm土层差异较大,但不同土层AWCD在两种林分之间均未达到显著差异(P0.05)。林分P2 0~10 cm土层AWCD、Shannon指数(H')、Simpson指数(D)、Mclntosh指数(U)和丰富度指数(S)均高于林分P1,林分P2的H'与D显著高于林分P1(P=0.03,P=0.02),但10~20 cm土层所有功能参数在P1与P2之间没有显著差异(P0.05)。0~10 cm土层,林分P1土壤微生物对醇类和糖类利用率较高,林分P2对糖类和酯类利用率较高。10~20 cm土层,林分P1对醇类利用率较高,林分P2对胺类和醇类利用率较高。冗余分析(RDA)显示,铵氮、林分胸高断面积、林分丰富度指数、土壤含水率、溶解性有机碳(DOC)及溶解性氮(DN)对微生物碳源利用影响较大。其中,铵氮、林分丰富度指数、含水率、DN几乎与所有碳源代谢呈正相关。林分结构因子除林分丰富度指数外均与所有碳源代谢呈负相关;蒙特卡罗置换实验显示林分胸高断面积与土壤微生物群落代谢呈显著负相关(r2=0.42,P=0.02)。以上结果说明亚热带北部常绿阔叶林土壤微生物群落代谢特征受林分结构的制约。
[Abstract]:In order to study the soil microbial community characteristics of different stands in subtropical evergreen broadleaved forest, Castanopsis eyreii Champ.Tutch.Tutch.were selected in the subtropical evergreen broad-leaved forest in Chawan Nature Reserve, Qimen County, Anhui Province. It is a single dominant species stand P1, with Cyclobalanopsis glauca. Thunb. Oerst. Oerst., Castanopsis carlesii and Pinus massoniana Lamb. The relationship between the functional diversity of soil microbial community and stand structure was studied by Biolog method. The results showed that the soil nutrients (ammonium nitrogen, nitrate nitrogen, soluble organic carbon nitrogen) of stand P2 were higher than that of stand P1, but there was no significant difference between them, and the soil nutrient content of 0 ~ 10 cm soil layer of two stands was obviously higher than that of 10 ~ 20 cm soil layer. After cultivation for 48 h, the AWCD of 0 ~ 10 cm and 10 ~ 20 cm soil layer of stand P2 was higher than that of P1, and there was a great difference in 0 ~ 10 cm soil layer, but there was no significant difference in AWCD between the two stands. In 10 cm soil layer of stand P20, the Shannon index of AWCD and Shannon index and the index of Simpson index are higher than that of stand P1, and the abundance index of stand P2 is significantly higher than that of stand P1, but all the function parameters of 1020cm soil layer are not significantly different between P1 and P2 (P 0.05N. 010 cm). The values of P 'and D of stand P2 are significantly higher than those of stand P 1, P 1, P 0. 03 and P 0. 02, but there is no significant difference between P 1 and P 2 in all the functional parameters of the soil layer (P 0. 05 and P 0. 010 cm). The soil microbial utilization ratio of alcohol and sugar was higher in stand P1, the utilization ratio of sugar and esters in stand P2 was higher than that in stand P2, the utilization rate of alcohol in stand P1 was higher than that in stand P2, and the utilization ratio of amines and alcohols in stand P2 was higher than that in stand P2. The results of redundancy analysis (RDA) showed that ammonium nitrogen, stand height sectional area, stand richness index, soil moisture content, dissolved organic carbon (DOC) and dissolved nitrogen (DN) had great effects on microbial carbon source utilization. Among them, ammonium nitrogen, stand richness index, moisture content and DN were positively correlated with the metabolism of all carbon sources. Except stand richness index, stand structure factor had negative correlation with all carbon source metabolism, Monte-Carlo replacement experiment showed that stand height cross-sectional area had significant negative correlation with soil microbial community metabolism, and there was a significant negative correlation between stand height area and soil microbial community metabolism. The above results indicate that the metabolic characteristics of soil microbial communities in the evergreen broadleaved forest in the northern subtropics are restricted by the stand structure.
【作者单位】：安徽农业大学林学与园林学院;
【基金】：国家自然科学基金项目(31370626;31070588) 国家“973”计划项目(2012CB416905)
【分类号】：S714.3

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