一种生物刺激素对大豆根际土壤微生物群落的影响

发布时间：2018-05-29 11:53

本文选题：生物刺激素 + 大豆　；参考：《大豆科学》2017年05期

【摘要】：为研究生物刺激素(二苯基脲磺酸钙)对大豆根际土壤微生物群落的影响,采用中华费氏根瘤菌或不同浓度的生物刺激素对汾豆78进行浸种,田间条件下播种后在大豆花期时采集大豆根际土壤,并通过对比改良SDS法、TSAI法、生工试剂盒法和Mobile试剂盒法,选取效果最佳的Mobile试剂盒法进行土壤细菌DNA提取,对目的片段16S V3区进行特异性扩增,扩增产物用于后续高通量测序分析。结果表明:5个试验组与CK组相比,试验组Shannon数值下降。处理S2、S3、S4和S5与CK相比,变形菌门、放线菌门和拟杆菌门相对含量增加;酸杆菌门和硬壁菌门相对含量减少。试验还发现在属的水平上,一些对大豆生长有益和促进固氮的功能菌种有增加趋势。生物刺激素使大豆根际土壤微生物多样性下降;可以促进根瘤菌的生长,增加根际土壤微生物中根瘤菌的含量;同时生物刺激素还可以促进根际土壤微生物中功能菌属的数量,不仅促进大豆固氮,还促进大豆生长发育。
[Abstract]:In order to study the effect of biostimulants (calcium diphenylurea sulfonate) on the microbial communities of soybean rhizosphere soil, Fen-dou 78 was soaked with Rhizobium flexneri or different concentrations of biostimulins. Soybean rhizosphere soil was collected at flowering stage after sowing under field conditions. The best Mobile kit was selected to extract soil bacterial DNA by comparing the improved SDS method with TSAI method, bioengineering kit method and Mobile kit method. The 16s V3 region of the target fragment was specifically amplified, and the amplified product was used for subsequent high throughput sequencing analysis. The results showed that the Shannon value of the five experimental groups was lower than that of CK group. Compared with CK, the relative contents of Proteus, Actinomycetes and Bacteroides in treatment S2S3S4 and S5 increased, while the relative contents of Acidobacterium and S5 decreased. It was also found that some functional strains which were beneficial to soybean growth and promoted nitrogen fixation tended to increase at the generic level. Biostimulins can decrease microbial diversity in soybean rhizosphere soil; promote the growth of rhizobia and increase the content of rhizobia in rhizosphere soil; at the same time, it can also promote the number of functional microbes in rhizosphere soil. Not only to promote soybean nitrogen fixation, but also to promote soybean growth and development.
【作者单位】：山西大学应用化学研究所;
【基金】：基金项目:林业公益性行业科研(201304326)
【分类号】：S154.3;S565.1

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