基于rDNA-ITS的中国外来香蕉穿孔线虫种群的系统发育分析

发布时间：2018-05-30 22:36

本文选题：香蕉穿孔线虫 + 系统发育　；参考：《华中农业大学学报》2017年06期

【摘要】：为揭示传入中国的香蕉穿孔线虫群体间的遗传差异,分析其亲缘和进化关系,明确中国香蕉穿孔线虫的来源和传入途径,分别扩增了多雌繁殖群体和相应种群的单雌繁殖群体后代rDNA-ITS,对扩增产物进行测序,通过序列比对分析其遗传变异,并构建系统发育树研究其亲缘和进化关系。结果表明:供试香蕉穿孔线虫20个种群的rDNA-ITS序列大小为705~713bp,序列同源性比较高,相似性在95%以上,序列变异率为0~7.5%;各群体ITS1和ITS2序列大小分别为273~276bp和151~152bp,各种群间的序列相似性分别达到98.58%和98.35%以上;单雌繁殖群体与相应的多雌繁殖群体的序列相似性非常高,有8个种群的2个繁殖群体序列相似性达到100%,其他种群的2个繁殖群体序列相似性达97%以上;基于ITS序列构建穿孔属线虫的系统发育树说明,所有香蕉穿孔线虫聚在一个大枝,分成4小分枝,其中寄主为红掌的RsW种群单独聚为一枝,遗传距离较远,寄主为绿宝石的RsH遗传距离相对其他种群也较远,寄主分别为天鹅绒竹芋和孔雀竹芋的RsP和RsS种群的遗传距离则相对较近,这说明传入中国的香蕉穿孔线虫种群可能直接来源是欧洲的竹芋科和天南星科观赏植物,而前者的最初来源可能是苏丹的香蕉,后者的最初地理来源较复杂。
[Abstract]:In order to reveal the genetic difference among the populations of Banana perforated nematode introduced into China, and to analyze its genetic and evolutionary relationship, the origin and the pathway of introduction of Banana perforated nematode in China were determined. The rDNA-ITSs of the polyfemale breeding population and the single female breeding population of the corresponding population were amplified, the amplified products were sequenced, the genetic variation was analyzed by sequence alignment, and phylogenetic tree was constructed to study the phylogenetic and evolutionary relationship of rDNA-ITSs. The results showed that the rDNA-ITS sequence of 20 populations of Banana perforated nematode was 705 ~ 713bp. the homology of the sequence was high, and the similarity was more than 95%. The sequence variation rate was 0 / 7.5.The ITS1 and ITS2 sequences of each population were 273~276bp and 151 ~ 152bprespectively, and the sequence similarity among different populations was 98.58% and 98.35% respectively, and the sequence similarity between the single female breeding population and the corresponding polyfemale breeding population was very high. The sequence similarity of two breeding populations of 8 populations was 100%, and that of 2 breeding populations of other populations was more than 97%. The phylogenetic tree of perforated nematode based on ITS sequence showed that all banana nematodes were clustered in one branch. There were 4 small branches, in which the RsW population of the host was a single branch, and the genetic distance of the host was longer than that of the other populations, and the genetic distance of the host RsH was also longer than that of the other populations. The genetic distance of the populations of RsP and RsS were relatively close, which indicated that the populations of Banana perforated nematodes introduced into China were probably directly originated from the European families of Phyllostachaceae and the ornamental plants of Araceae. The original source of the former may be Sudanese bananas, the latter's original geographical source is more complex.
【作者单位】：华南农业大学农学院/华南农业大学植物检疫线虫检测与防疫研究中心;山东省东营市妇女联合会;全国农业技术推广服务中心;
【基金】：国家自然科学基金项目(30471163;30671366) 农作物病虫鼠害疫情监测与防治项目(10162130108235047)
【分类号】：S432.45

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