固态发酵中2种微生物降解玉米秸秆效果的对比研究

发布时间：2018-05-30 23:30

本文选题：固态发酵 + 玉米秸秆　；参考：《农业环境科学学报》2017年10期

【摘要】：为探究哈茨木霉(Trichoderma harzianum)和枯草芽孢杆菌(Bacillus subtilis)这2种菌株在固态发酵条件下降解玉米秸秆的效果,通过25 d的室内发酵培养试验对固态发酵中添加哈茨木霉和枯草芽孢杆菌后玉米秸秆的有机碳、纤维素、木质素、纤维素酶活、木聚糖酶活以及β-葡萄糖苷酶活变化进行对比研究。结果表明:发酵第10~16 d时,哈茨木霉和枯草芽孢杆菌处理的玉米秸秆分解速率、纤维素酶活、木聚糖酶活和β-葡萄糖苷酶活均达到最高,发酵25 d后,2种微生物处理的玉米秸秆分别累积降解了21.79%和20.12%,说明两者的降解效果差异不大;与枯草芽孢杆菌处理相比,哈茨木霉处理的秸秆剩余量、有机碳含量、剩余秸秆有机碳总量分别降低了1.67%、0.26%和1.80%,秸秆纤维素降解率、秸秆木质素降解率、纤维素酶活、木聚糖酶活和β-葡萄糖苷酶活分别升高了6.99%、6.54%、0.7 FPU·m L~(-1)、0.04 IU·m L~(-1)和9.26 IU·m L~(-1),说明添加哈茨木霉和枯草芽孢杆菌均可以降解秸秆,但两者对玉米秸秆的降解效果差异不明显。
[Abstract]:In order to study the degradation effect of Trichoderma harzianum (Trichoderma harzianum) and Bacillus subtilis (Bacillus subtilis) in solid state fermentation, The organic carbon, cellulose, lignin and cellulase activity of corn straw after adding Trichoderma harzii and Bacillus subtilis to solid fermentation were studied in 25 days. The changes of xylanase activity and 尾-glucosidase activity were compared. The results showed that the decomposition rate, cellulase activity, xylanase activity and 尾 -glucosidase activity of corn straw treated by Trichoderma harzianum and Bacillus subtilis reached the highest at 10 ~ 16 days after fermentation. After 25 days of fermentation, the corn straw treated by Trichoderma harzii had a cumulative degradation rate of 21.79% and 20.12% respectively, indicating that the degradation effect was not different between the two treatments, and the residual amount and organic carbon content of straw treated by Trichoderma harziformis were similar to those of Bacillus subtilis treatment. The total organic carbon content of residual straw decreased by 0.26% and 1.80%, respectively. The degradation rate of straw cellulose, the degradation rate of straw lignin, the activity of cellulase were also decreased. The activities of xylanase and 尾 -glucosidase increased 6.99 ~ 6.54 ~ (0.7 FPU / L ~ (-1) and 9.26 IU / L ~ (-1) respectively, indicating that both Trichoderma harzii and Bacillus subtilis could degrade the straw, but there was no significant difference between them.
【作者单位】：吉林农业大学资源与环境学院;吉林大学生命科学学院;
【基金】：国家自然科学基金项目(41571231) 国家重点研发计划项目(2016YFD0200304) 吉林省秸秆综合利用平台项目(2014C-1)~~
【分类号】：S216.2;X712

【相似文献】