黄河三角洲刺槐根际与非根际细菌结构及多样性

发布时间：2018-10-22 07:33

【摘要】：为精确分析黄河三角洲刺槐根际与非根际土壤细菌群落定殖情况,本研究采用高通量测序方法对刺槐根际与非根际土壤细菌结构及多样性进行了研究。研究表明,根际土壤细菌共有36门214属,非根际土壤细菌共有33门153属。变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)细菌丰度超过15%,是根际与非根际土壤中的优势菌落。根际与非根际土壤中酸杆菌门、硝化螺旋菌门(Nitrospirae)丰度差异显著。根际土壤中红游动菌属(Rhodoplanes)、溶杆菌属(Lysobacter)、热单胞菌属(Thermomonas)、链霉菌属(Streptomyces)及非根际土壤中红游动菌属、溶杆菌属、链霉菌属、Kaistobacter细菌丰度超过4%。根际土壤中固氮菌丰度显著高于非根际土壤,解磷、解钾细菌丰度差异不显著。根际与非根际土壤细菌Chao丰富度分别为2 054、2 376,差异显著。根际与非根际土壤细菌之间的权重(Weighted Unifrac)距离在0.12~0.25之间。综上所述,黄河三角洲刺槐根际与非根际土壤细菌结构具有一定差异,多样性差异显著。
[Abstract]:In order to accurately analyze the colonization of rhizosphere and non-rhizosphere soil bacterial communities of Robinia pseudoacacia in the Yellow River Delta, the bacterial structure and diversity of Robinia pseudoacacia rhizosphere and non-rhizosphere soil were studied by high-throughput sequencing. The results showed that there were 36 phyla 214 genera in rhizosphere soil and 33 phyla 153 genera in non rhizosphere soil. Proteus (Proteobacteria), actinomycetes (Actinobacteria), actinobacteria (Acidobacteria) bacteria abundance is more than 15 and is the dominant colony in rhizosphere and non-rhizosphere soil. There were significant differences in (Nitrospirae) abundance between rhizosphere soil and non-rhizosphere soil. In rhizosphere soil, Rhizobium, (Rhodoplanes), (Lysobacter), (Lysobacter), Streptomyces, (Streptomyces), Lysobacillus, Streptomyces, and Kaistobacter are more than 4. The abundance of nitrogen-fixing bacteria in rhizosphere soil was significantly higher than that in non-rhizosphere soil. The Chao richness of bacteria in rhizosphere and non-rhizosphere soil was 2 054 (2376), respectively. The (Weighted Unifrac) distance between rhizosphere and non-rhizosphere soil bacteria was 0.120.25. In conclusion, the soil bacterial structure of Robinia pseudoacacia in the Yellow River Delta is different from that in non-rhizosphere soil, and the diversity is significant.
【作者单位】：山东农业大学林学院山东农业大学生态与环境实验室;中国科学院西北生态环境资源研究院;中国科学院大学;
【基金】：国家自然科学基金项目(30970499) 世界银行贷款项目(SEAP-KY-2)共同资助~~
【分类号】：S714.3;S792.27

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