不同磷水平下接种AM真菌对采煤塌陷区土壤GRSP量及玉米生长的影响
发布时间：2018-10-22 08:48
【摘要】：煤炭大量开采造成地面塌陷,使土壤结构破坏,肥力下降,阻碍作物正常生长和对养分的吸收。丛枝菌根真菌(Arbuscular mycorrhizal fungi,AM真菌)在恢复退化的农林生态系统中具有重要的生理功能,尤其促进对土壤磷的吸收。通过盆栽试验,研究4个施磷水平(0、25、50和100 mg/kg)下单接种摩西球囊霉(Glomus mosseae,G.m)、双接种幼套球囊霉(Glomus etunicatum,G.e)与摩西球囊霉对采煤塌陷区土壤球囊霉素相关土壤蛋白(GRSP)、玉米生长及地上部磷吸收的影响。结果表明,接种与磷水平及他们之间的交互作用对AM真菌丛枝丰度、株高、茎粗和易提取球囊霉素相关土壤蛋白(EE-GRSP)量具有显著影响(P0.05);接种处理AM真菌丛枝丰度在0.29%~25.22%之间,同一磷水平双接种处理显著高于单接种处理(P0.05)。而且GRSP量与AM真菌丛枝丰度表现出显著的二次曲线相关。相同磷水平下,总球囊霉素相关土壤蛋白(T-GRSP)和易提取球囊霉素相关土壤蛋白(EE-GRSP)量均呈双接种≥单接种不接种,同一接种处理GRSP量随磷水平提高先升后降。虽然单接种和不接种处理在不同磷水平间对玉米生长的影响差异不显著,但是双接种处理显著提高了玉米株高、茎粗、地上部干质量及地上部吸磷量,表现为50、100 mg/kg磷水平显著高于0、25 mg/kg磷水平;相关性分析表明,AM真菌丛枝丰度与GRSP量、株高、茎粗、地上部磷量呈显著正相关。
[Abstract]:Large amount of coal mining results in ground collapse, soil structure destruction and fertility decline, which hinder the normal growth of crops and nutrient absorption. Arbuscular mycorrhizal fungi (Arbuscular mycorrhizal fungi,AM) play an important role in the restoration of degraded agroforestry ecosystem, especially in promoting the absorption of soil phosphorus. Through pot experiments, The effects of four phosphorus application levels (0 ~ 25 ~ (50) and 100 mg/kg) on the growth and phosphorus uptake of (GRSP), corns, a soil protein associated with Bleomycin, were studied by placing orders for inoculation of Glomus mosseae,G.m and double inoculation of Glomus etunicatum,G.e and Mosaic Balloon in the soil of coal mining collapse area. The results showed that inoculation and phosphorus level and their interaction had significant effects on the AM fungi branch abundance, plant height, stem diameter and the amount of EE-GRSP (P0.05), and the branching abundance of AM fungi was between 0.29% and 25.22%. The treatment of double inoculation at the same phosphorus level was significantly higher than that of single inoculation (P0.05). Moreover, the GRSP content was significantly correlated with the branch abundance of AM fungi. Under the same phosphorus level, the amount of total balloon mycin associated soil protein (T-GRSP) and easy to extract balloon mycin associated soil protein (EE-GRSP) were all double inoculated 鈮，
本文编号：2286686
[Abstract]:Large amount of coal mining results in ground collapse, soil structure destruction and fertility decline, which hinder the normal growth of crops and nutrient absorption. Arbuscular mycorrhizal fungi (Arbuscular mycorrhizal fungi,AM) play an important role in the restoration of degraded agroforestry ecosystem, especially in promoting the absorption of soil phosphorus. Through pot experiments, The effects of four phosphorus application levels (0 ~ 25 ~ (50) and 100 mg/kg) on the growth and phosphorus uptake of (GRSP), corns, a soil protein associated with Bleomycin, were studied by placing orders for inoculation of Glomus mosseae,G.m and double inoculation of Glomus etunicatum,G.e and Mosaic Balloon in the soil of coal mining collapse area. The results showed that inoculation and phosphorus level and their interaction had significant effects on the AM fungi branch abundance, plant height, stem diameter and the amount of EE-GRSP (P0.05), and the branching abundance of AM fungi was between 0.29% and 25.22%. The treatment of double inoculation at the same phosphorus level was significantly higher than that of single inoculation (P0.05). Moreover, the GRSP content was significantly correlated with the branch abundance of AM fungi. Under the same phosphorus level, the amount of total balloon mycin associated soil protein (T-GRSP) and easy to extract balloon mycin associated soil protein (EE-GRSP) were all double inoculated 鈮，
本文编号：2286686
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