水稻根际土壤溶磷菌的分离、鉴定及对水稻的促生作用

发布时间：2018-11-05 09:15

【摘要】：【目的】为明确水稻根际溶磷菌株溶磷能力及其对水稻植株及根际土壤磷含量的影响。【方法】利用溶磷圈法从水稻根际土壤中分离获得具有较强溶解Ca3(PO4)2能力的2株细菌NDW1和NDW3,根据16S r DNA对菌株进行鉴定。以NBRIP为基础培养基,通过正交试验对2株菌株利用氮源、碳源及初始p H进行优化,鉴定菌株的吲哚乙酸(IAA)分泌量,研究溶磷菌对水稻的促生作用,以及对土壤速效磷和水稻幼苗全磷含量的影响。【结果】菌株NDW1和菌株NDW3分别被鉴定为Enterobacter sp.和Serratia sp.,2株菌株溶磷的最佳条件组合均为葡萄糖、蛋白胨及初始p H 6。2株菌株24 h内最高溶磷量分别为294.95和312.93μg·m L-1,且都可分泌IAA。土培和沙培条件下,溶磷菌NDW3对水稻株高、根长、最大叶长及地上干质量都有明显的促进作用,并且NDW3在2种种植条件下均显著增加了根际土壤速效磷及水稻植株全磷含量。【结论】从水稻根际土壤分离获得2株溶磷能力较好的细菌菌株Enterobacter sp.NDW1和Serratia sp.NDW3,菌株NDW3对水稻的促生作用强于菌株NDW1。
[Abstract]:[objective] to determine the phosphorus solubilizing ability of rice rhizosphere phosphorus solubilizing strain and its effect on phosphorus content in rice plant and rhizosphere soil. [methods] Ca3 (PO4) with strong solubility was obtained from rice rhizosphere soil by phosphorus dissolving circle method. 2. NDW1 and NDW3, The strain was identified according to 16s r DNA. Based on NBRIP culture medium, two strains were optimized by using nitrogen source, carbon source and initial pH to identify the (IAA) secretion of indoleacetic acid, and the effect of phosphorus solubilizing bacteria on rice growth was studied. And the effect on soil available phosphorus and total phosphorus content of rice seedlings. [results] strain NDW1 and strain NDW3 were identified as Enterobacter sp., respectively. The optimum conditions for phosphorus solubilization of Serratia sp.,2 strain and peptone strain were glucose, and the highest phosphorus solubilization amount of peptone and initial pH 6.2 strain were 294.95 and 312.93 渭 g mL ~ (-1) in 24 h, respectively, and all of them could secrete IAA.. Under the conditions of soil culture and sand culture, phosphorus soluble bacteria (NDW3) significantly promoted plant height, root length, maximum leaf length and aboveground dry mass of rice. NDW3 significantly increased the available phosphorus in rhizosphere soil and the total phosphorus content in rice plants under both planting conditions. [conclusion] two bacterial strains, Enterobacter sp.NDW1 and Serratia sp.NDW3, with better phosphorus solubilization ability were obtained from rice rhizosphere soil. The effect of strain NDW3 on rice growth is stronger than that of strain NDW1..
【作者单位】：吉林农业大学农学院;吉林农业大学生命科学学院;
【基金】：国家自然科学基金(31201687)
【分类号】：S154.3;S511

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