苹果树根际高效解钾菌的筛选及鉴定

发布时间：2018-11-12 11:05

【摘要】：为探明苹果树根际解钾菌的种群特征和解钾性能,从苹果树根际土壤中分离获得解钾能力较强的高效解钾菌。本研究以钾长石为唯一钾源,分离获得118株具有解钾活性的菌株,重复片段PCR基因指纹分析(Repetitive-element PCR,rep-PCR)聚为29个类群。利用火焰分光光度法测定菌株解钾能力,获得5株高效解钾菌,平均解钾活性达到41.47mg·L-1,其中K105的解钾能力最强,有效态钾增长23.09%。采用H2O2消煮后测得的K+浓度升高,有效态钾增长最高达31.22%。采用形态特征观察、生理生化特性检测和基于16S r RNA基因序列的系统发育分析对高效解钾菌株进行鉴定。结果表明:K1为不动杆菌属(Acinetobacter sp.)、K98、K105和K115为假单胞菌属(Pseudomonas sp.)、K168为芽孢杆菌属(Bacillus sp.)。研究结果可为开辟新型高效解钾菌,为连作土壤的改良和苹果专用微生物菌肥的开发提供依据。
[Abstract]:In order to find out the population characteristics and properties of kalium lysozyme isolated from apple tree rhizosphere soil, a high potassium hydrolytic bacteria was isolated from the rhizosphere soil of apple tree. In this study, 118 strains with potassium hydrolysis activity were isolated from potassium feldspar as the sole potassium source. The repeat fragment PCR gene fingerprinting (Repetitive-element PCR,rep-PCR) was clustered into 29 groups. Using flame spectrophotometry to determine the ability of potassium hydrolysis of the strain, 5 strains of high efficiency potassium hydrolyzing bacteria were obtained. The average activity of potassium hydrolysis reached 41.47mg L -1, in which K105 had the strongest ability to dissolve potassium, and the available potassium increased by 23.09%. The K concentration measured by H2O2 was increased and the available potassium increased to 31.22. Morphological observation, physiological and biochemical characteristics detection and phylogenetic analysis based on 16s r RNA gene sequence were used to identify the highly efficient Kaliolytic bacteria strains. The results show that K1 is Acinetobacter (Acinetobacter sp.), K98 K105 and K115 is Pseudomonas (Pseudomonas sp.), K168 and Bacillus (Bacillus sp.). The results can provide a basis for the development of a new type of high-efficient potassium hydrolytic bacteria and for the improvement of continuous cropping soil and the development of special microbial fertilizer for apple.
【作者单位】：北京林业大学生物科学与技术学院;中国农业科学院农业环境与可持续发展研究所;
【基金】：北京林业大学青年科技启动基金(BLX2014-25) 流域农业面源污染防控整装技术与农业清洁流域示范(2015ZX07103-007)
【分类号】：S154.3;S661.1

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