小菜蛾幼虫肠道细菌桃色欧文氏菌的代谢表型组学分析（英文）

发布时间：2018-12-11 16:46

【摘要】：【目的】肠道细菌桃色欧文氏菌Erwinia persicina是小菜蛾Plutella xylostella幼虫肠道的优势细菌,本研究旨在阐明桃色欧文氏菌的代谢表型特征。【方法】采用BIOLOG细胞表型芯片技术系统研究了桃色欧文氏菌的细胞表型;采用PM 1-10代谢板,对桃色欧文氏菌的950种代谢表型进行了测定。【结果】桃色欧文氏菌能代谢39.47%的碳源、89.74%的氮源、100%的硫源和100%的磷源;高效代谢的碳源为有机酸类和碳水化合物类,高效代谢的氮源为氨基酸类。该肠道细菌表现出261种不同的氮源代谢通路和95种生物合成通路。桃色欧文氏菌具有广泛的适应性,能在分别具有高达9%氯化钠、4%氯化钾、5%硫酸钠、20%乙二醇、6%甲酸钠、2%尿素、6%乳酸钠、200mmol/L磷酸钠(p H 7.0)、20 mmol/L苯甲酸钠(p H 5.2)、100 mmol/L硫酸铵(p H 8.0)、100 mmol/L硝酸钠和100 mmol/L亚硝酸钠的渗透溶液中正常代谢;其适应p H值范围为4.5~10,最适7.0。在多种氨基酸的作用下,桃色欧文氏菌均表现出脱羧酶和脱氨酶活性。【结论】桃色欧文氏菌的代谢特征增加了我们对该肠道细菌,特别是其与宿主昆虫的互作及其在肠道环境中的适应性的认识,同时表明通过降低桃色欧文氏菌密度来防治小菜蛾的可能性。
[Abstract]:[objective] intestinal bacteria Erwinia persicina is the dominant bacteria in the intestine of Plutella xylostella larvae of Plutella xylostella. The purpose of this study was to elucidate the metabolic phenotypic characteristics of Erwin peach. [methods] the phenotype of Irvine peach was studied by BIOLOG cell phenotypic microarray technique. The PM 1-10 metabolic plate was used to determine the phenotype of 950species of Erwinia peachcolor. [results] 39.47% of carbon source, 89.74% of nitrogen source, 100% of sulfur source and 100% of phosphorus source were metabolized by Irvine peach color. The carbon sources were organic acids and carbohydrates, and the nitrogen sources were amino acids. The intestinal bacteria showed 261 different nitrogen metabolic pathways and 95 biosynthesis pathways. Erwinia peachcolor has a wide range of adaptations. It can be used as high as 9% sodium chloride, 4% potassium chloride, 5% sodium sulfate, 20% ethylene glycol, 6% sodium formate, 2% urea, 6% sodium lactate, respectively. 200mmol/L sodium phosphate (p H 7.0), 20 mmol/L sodium benzoate (p H 5.2), 100 mmol/L ammonium sulfate (p H 8.0), 100 mmol/L sodium nitrate and 100 mmol/L sodium nitrite in osmotic solution. The pH value range of adaptation was 4.5 ~ 10, and the optimum value was 7.0. The activities of decarboxylase and deaminase were observed by Erwinia peachoniae under the action of many amino acids. [conclusion] the metabolic characteristics of Erwinia peachoniae increased the activity of the intestinal bacteria. In particular, the interaction with host insects and their adaptability in the intestinal environment were recognized, and the possibility of controlling Plutella xylostella by reducing the density of Erwinia peachurensis was also indicated.
【作者单位】：贵州大学昆虫研究所;贵州省植物保护研究所;
【基金】：农业昆虫与害虫防治贵州省研究生卓越人才计划(黔教研合ZYRC字[2013]010) 贵州省科技基金项目([2015]2102)
【分类号】：S433.4;Q93

【参考文献】