酸性土壤中接种耐酸根瘤菌对豆科植物根际微生态的影响
发布时间：2019-07-27 09:39
【摘要】：【目的】接种耐酸豆科根瘤菌可以提高豆科植物耐酸能力。应用PLFA等方法研究接种耐酸根瘤菌对根际土壤微生态的综合影响,从土壤角度阐明接种耐酸根瘤菌缓解土壤酸度对豆科植物胁迫的机制。【方法】盆栽条件下分别向种植于p H 4.8酸性土壤中的葛藤和苜蓿植株相应接种耐酸葛藤根瘤菌068、389、390与耐酸苜蓿根瘤菌91512、91522、91532,于接种后30 d、60 d、90 d、120 d、150 d取样,分析耐酸根瘤菌对土壤养分、可培养微生物、微生物群落结构多样性的影响。【结果】1)接种耐酸葛藤和苜蓿根瘤菌处理后,根际土壤p H由4.8显著提高至6.0左右,有效磷、速效钾、铵态氮、硝态氮及有机质含量的增加效果显著(P0.05),至120 d达到最高。120 d样品的有效磷、铵态氮、硝态氮及有机质含量分别为23.16～48.68 mg/kg、61.21～81.96mg/kg、65.05～86.38 mg/kg和11.85～12.87 g/kg,分别比未接种对照提高24.8%～162.4%、16.6%～56.2%、145.4%～225.8%、1.4%～10.1%;2)接种苜蓿和葛藤耐酸根瘤菌后土壤中可培养微生物数量显著提高(P0.05),土壤中可培养细菌、真菌和放线菌数量在120 d达到峰值,比同期未接种对照分别提高了61.5%～348.4%、3.4%～441.7%和18.9%～255.2%,分别达到48.00×10~2～133.3×10~7、20.11×10~4～155.9×10~4和3.21×10~4～9.59×10~4 cfu/g鲜土;3)PLFA分析表明,虽然接种耐酸葛藤、苜蓿根瘤菌处理的根际土壤的特征脂肪酸种类数与未接种处理相比并无明显差异,但接种葛藤或苜蓿根瘤菌均明显降低了根际土壤中异构PLFA/反异构PLFA值,显示土壤根际微生态的稳定性得到提高。【结论】在种植豆科植物的酸性土壤中接种相应耐酸根瘤菌能显著提高根际土壤p H,提高根际土壤有机质含量和速效氮磷养分含量,缓解土壤酸性对根际微生物的胁迫。
[Abstract]:Objective to improve the acid tolerance of leguminous plants by inoculating acid-tolerant leguminous Rhizobium. PLFA and other methods were used to study the comprehensive effect of inoculating acid-tolerant Rhizobium on the microecology of rhizosphere soil, and to elucidate the mechanism of inoculating acid-tolerant Rhizobium to alleviate the stress of leguminous plants by soil acidity. [methods] under pot culture conditions, the acid-tolerant Gegeraria and Medicago sativa plants planted in pH 4.8 acid soil were inoculated with acid-resistant Gegeraria lobata 068389390 and acid-tolerant Medicago sativa 91512, 91522, 91532, respectively, 30 days, 60 days, 90 days, 120 days, 30 days, 60 days, 90 days, 120 days, 90 days and 120 days, respectively. The effects of acid-tolerant Rhizobium on soil nutrients, culturable microorganisms and microbial community structure diversity were analyzed by 150 d sampling. [results] 1) after inoculating with acid-tolerant Pueraria and Medicago sativa Rhizobium, the pH of rhizosphere soil increased significantly from 4.8 to 6.0, and the contents of available phosphorus, available potassium, ammonium nitrogen, nitrate nitrogen and organic matter increased significantly (P 0.05). The contents of available phosphorus, ammonium nitrogen, nitrate nitrogen and organic matter in 120 d samples were 23.16 卤48.68 mg/kg,61.21~81.96mg/kg,65.05~86.38 mg/kg and 11.85 脳 12.87 g 路kg ~ (- 1), respectively, which were 24.8% ~ 162.4%, 16.6% ~ 56.2%, 145.4% ~ 225.8% and 1.4% ~ 10.1%, respectively, which were 24.8% ~ 162.4%, 16.6% ~ 56.2%, 145.4% ~ 225.8% and 1.4% ~ 10.1%, respectively. 2) the number of culturable microorganisms in soil was significantly increased after inoculating Medicago sativa and Rhizobium graminearum (P 0.05). The number of culturable bacteria, fungi and actinomycetes in soil reached the peak at 120 days, which was 61.5% 鈮，
本文编号：2519923
[Abstract]:Objective to improve the acid tolerance of leguminous plants by inoculating acid-tolerant leguminous Rhizobium. PLFA and other methods were used to study the comprehensive effect of inoculating acid-tolerant Rhizobium on the microecology of rhizosphere soil, and to elucidate the mechanism of inoculating acid-tolerant Rhizobium to alleviate the stress of leguminous plants by soil acidity. [methods] under pot culture conditions, the acid-tolerant Gegeraria and Medicago sativa plants planted in pH 4.8 acid soil were inoculated with acid-resistant Gegeraria lobata 068389390 and acid-tolerant Medicago sativa 91512, 91522, 91532, respectively, 30 days, 60 days, 90 days, 120 days, 30 days, 60 days, 90 days, 120 days, 90 days and 120 days, respectively. The effects of acid-tolerant Rhizobium on soil nutrients, culturable microorganisms and microbial community structure diversity were analyzed by 150 d sampling. [results] 1) after inoculating with acid-tolerant Pueraria and Medicago sativa Rhizobium, the pH of rhizosphere soil increased significantly from 4.8 to 6.0, and the contents of available phosphorus, available potassium, ammonium nitrogen, nitrate nitrogen and organic matter increased significantly (P 0.05). The contents of available phosphorus, ammonium nitrogen, nitrate nitrogen and organic matter in 120 d samples were 23.16 卤48.68 mg/kg,61.21~81.96mg/kg,65.05~86.38 mg/kg and 11.85 脳 12.87 g 路kg ~ (- 1), respectively, which were 24.8% ~ 162.4%, 16.6% ~ 56.2%, 145.4% ~ 225.8% and 1.4% ~ 10.1%, respectively, which were 24.8% ~ 162.4%, 16.6% ~ 56.2%, 145.4% ~ 225.8% and 1.4% ~ 10.1%, respectively. 2) the number of culturable microorganisms in soil was significantly increased after inoculating Medicago sativa and Rhizobium graminearum (P 0.05). The number of culturable bacteria, fungi and actinomycetes in soil reached the peak at 120 days, which was 61.5% 鈮，
本文编号：2519923
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