大豆生物活性肽对仔猪生长性能的影响及其机理的研究

  本文关键词：大豆生物活性肽对仔猪生长性能的影响及其机理的研究，由笔耕文化传播整理发布。

        本研究是以大豆生物活性肽为原料，着重研究了大豆肽对仔猪的生产性能、腹泻发生率、血清学指标的影响，并且从免疫学、肠道组织形态学和肠道微生物组成三个方面对大豆肽的影响机制进行了初步探讨。 一、大豆生物活性肽对断奶仔猪生产性能的影响 试验共选取初始体重相近的35±2日龄断奶仔猪240头(杜×长×大)，随机分为5个处理，每个处理6个重复，每个重复8头猪(各重复公母比例相同)。分别饲喂空白日粮、含75mg／kg金霉素对照日粮、1000mg／kg、3000mg／kg及5000mg／kg大豆生物活性肽的试验日粮。饲养期为25天，记录每个处理的腹泻情况，计算日增重、平均日采食量，计算饲料转化效率。第25天时从颈静脉采血，用以测定血清蛋白质、血糖、血清尿素氮等生化指标。试验表明，大豆生物活性肽添加到仔猪日粮中后，可以显著提高仔猪的饲料转化效率(P＜0.05)，同时降低腹泻率(P＜0.05)。由本试验得出的大豆生物活性肽在断奶仔猪料中的适宜添加量为3000mg／kg。大豆生物活性肽可以提高仔猪血清中的总蛋白(P＜0.01)、球蛋白(P＜0.01)的含量。以3000mg／kg浓度添加时可以提高血糖(P＜0.01)和血清尿素氮含量(P＜0.05)。 二、大豆生物活性肽对育成初期猪只生产性能的影响 选取健康、体格接近的70±3日龄的猪只192头，按体重和性别随机分配到三个处理中，每个处理4个重复，每个重复16头猪，分别饲喂空白日粮、3000mg／kg大豆肽及75mg／kg金霉素，饲养期为30天。结果表明，在育成初期阶段，抗生素与肽的添加对日增重、饲料转化效率已不再产生显著的促进作用(P＞0.05)，但是抗生素和肽仍然表现出一定的促生长趋势，其中3000mg／kg肽组与空白组相比，料重比降低了约18.2％，效果优于抗生素，故仍然可以考虑添加，这样有利于提高肉质，减少药物带来的不良影响。 三、大豆生物活性肽促进仔猪生产性能机理的研究 本试验分别从免疫学、肠道组织形态学以及微生物学三个方面探讨了大豆活性肽的作用机理，试验使用的是与试验一相同日龄的猪只，在试验期结束时(25天)，进行采血和屠宰，用以测定抗体水平、肠绒毛高度、隐窝深度及肠道微生物组成。试验结果表明，添加大豆生物活性肽的试验组，其对牛血清白蛋白产生的抗体水平有依浓度增加而提高的趋势，并且均高于两个对照组，接近显著性水平(P=0.0501)。3000mg／kg肽水平可以提高部分肠段的绒毛高度，并加深部分隐窝深度，，但显著性不一。同时，大豆生物活性肽有一定的抑菌效果(主要针对耗氧型细菌)，其抑制沙门氏菌的效果在结肠已经显著高于抗生素(P＜0.01)，而对乳酸菌，在结肠则显现出了极显著的促进作用(P＜0.01)，但同时又显著抑制了乳酸菌在盲肠的数量(P＜0.01)。

    This research centered on the effects of soybean peptides on growth performance of weanling piglets. Furthermore, the mechanism of these effects was investigated on aspects of immunology, intestinal histology and gut micro-flora.Experiment 1: Effects of soybean oligopeptides (SBP) on growth performance of weanling pigs240 35-days-old cross-bred (DoricXLandraceXLarge White) weaned piglets with initial weight at 8.78 + 0.98kg were randomly allotted to five treatments with six replicates per treatment and eight pigs per pen (four gilts and four barrows) at similar initial body weight. Basal diet was fed to the control group and basal diet+75mg/kg Aureomycin, 1000mg/kg SBP, 3000mg/kg SBP, 5000mg/kg SBP supplement to other experimental groups, respectively. Results showed that addition of 3000mg/kg SBP significantly increased ADG and decreased feed/gain efficiency (P<0.05) while diarrhea incidences were significantly decreased by the addition of 1000mg/kg SBP (P<0.05) . As for ADFI, the differences among all groups were not significant. Experiment 2: Effects of SBP on growth performance of young growing pigs192 70-days-old cross-bred (DoricXLandraceXLarge White) young growing pigs with initial weight at 16.68 +0.67kg were randomly allotted to three treatments with four replicates per treatment and sixteen pigs per pen (four gilts and four barrows) at similar initial body weight. Basal diet was fed to the control group and basal diet +75mg/kg Aureomycin, 3000mg/kg SBP supplement to other experimental groups, respectively. Results showed that addition of 3000mg/kg SBP increased ADG and decreased feed/gain efficiency by a certain amount which was better than the other two groups on a non-significant level (P>0.05). Experiment 3: Mechanism of the growth-promoting effects on weanling pigs of SBPThe design of this experiment was the same as that of experiment 1, and aimed at discovering the mechanism of the effects showed in the former two experiments by means of immunology, intestinal histology and gut microbiology. The pigs were inoculated by BSA twice to ensure the animals could produce high levels of antibodies in the blood. Four pigs from each of the control group, Aureomycin group and 3000mg/kg SBP group were slaughtered after 25 days of experiment. The results showed that SBP added up to the level of blood antibodies and might affect increasingly or decreasingly to different stains in cecum and colon. The main inhibiting effects appeared on aerobes (E. coli and salmonella), while lactobacilli were promoted in the colon. At the same time, intestinal mucosal villi were heightened and crypts were deepened significantly in the SBP groups, and the percentage of goblet cell was increased ultra significantly as well.

大豆生物活性肽对仔猪生长性能的影响及其机理的研究

第一章 绪论8-22    1 肽营养理论的提出与发展的简要历程8-10    2 肽营养的研究与应用10-17    3 活性肽生物制品17-21    4 本研究的内容和意义21-22第二章 大豆肽对断奶仔猪生产性能影响的研究22-31    1 试验材料与方法22-23    2 试验结果与分析23-28    3 讨论28-30    4 小结30-31第三章 大豆肽对育成初期猪只生长性能影响的研究31-34    1 试验材料与方法31-32    2 试验结果与分析32-33    3 讨论33    4 小结33-34第四章 大豆生物活性肽提高仔猪生产性能机理的研究34-51    1 大豆生物活性肽对仔猪抗体水平的影响34-35    2 大豆生物活性肽对仔猪肠道组织形态学的影响35-43    3 大豆生物活性肽对仔猪结肠、盲肠微生物组成的影响43-46    4 讨论46-50    5 小结50-51第五章 本试验主要结论及有待进一步研究的问题51-52参考文献52-61附录61-68致谢68-69作者简历69

  本文关键词：大豆生物活性肽对仔猪生长性能的影响及其机理的研究，由笔耕文化传播整理发布。