液态中交叠微球的三维纳米级位置测量方法

发布时间：2018-05-23 15:03

本文选题：测量 + 数字全息　；参考：《光学学报》2017年08期

【摘要】：微球跟踪技术是生物学动态过程的重要研究工具之一。为获取相关生物学信息,需要对微纳米尺度的微球进行三维位置的测量。在实际测量中,由于液态环境中微球间及微球与其他物质间存在相互作用力,微球图像交叠的现象难以避免。交叠微球图像会导致圆环特征的交错,采用传统方法难以实现特征识别,进而无法对其定位。针对这一问题,提出了全息重构聚焦法(HRFM),通过精确定位微球的横向位置,获取其三维位置。实验结果表明HRFM定位微球位置较传统方法更为精确,且尤其适用于交叠微球位置的测量,对单个微球和交叠微球的测量均能达到2nm的三维位移分辨力。
[Abstract]:Microsphere tracking technology is one of the most important research tools in biological dynamic process. In order to obtain related biological information, micro and nano scale microspheres need to be measured in three-dimensional position. In the actual measurement, the overlapping of microspheres is difficult to avoid due to the interaction force between microspheres and microspheres and other substances in the liquid environment. The overlapping microspheres can lead to the interlacing of the ring features. It is difficult to identify the features by traditional methods and can not locate them. In this problem, a holographic refocusing and focusing method (HRFM) is proposed to locate the three-dimensional position of the microspheres accurately. The results show that the location of the HRFM positioning microsphere is more than the traditional method. It is accurate and suitable for measuring the position of overlapping microspheres. The measurement of single microspheres and overlapping microspheres can reach 2nm's three-dimensional displacement resolution.
【作者单位】：天津大学精密仪器与光电子工程学院;天津农学院工程技术学院;
【基金】：国家自然科学基金(51375340) 天津市自然科学基金(15JCZDJC31600)
【分类号】：O438.1;TP391.41

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