高剂量电离辐射对恒河猴器官组织病理改变及相关细胞因子表达的影响

发布时间：2018-05-25 05:33

本文选题：电离辐射 + 组织病理变化　；参考：《农业生物技术学报》2017年11期

【摘要】：放射治疗已成为治疗肿瘤的一种重要方法,但在治疗同时,高剂量的电离辐射会对机体各个组织器官造成损伤。为探讨高剂量电离辐射对恒河猴(Macaca mulatta)心、肝、脾、肺、肾及肠道组织的病理性损伤及相关细胞因子表达的影响,本研究用辐射能量9.5 Gy的60Coγ对3只雄性恒河猴照射4 h,于辐射后15 d处死并采集相关器官组织,采用苏木精-伊红染色(hematoxylin-eosin staining,HE)观察心、肝、脾、肺、肾及肠道组织病理损伤情况,同时采用免疫组化和图像分析技术检测各组织内肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-6(interleukin-6,IL-6)、IL-10、IL-2和转化生长因子β1(transforming growth factorβ1,TGF-β1)的表达。结果表明,辐射后15 d,跟对照相比,恒河猴心、肝、脾、肺及肾组织发生明显病理变化,而且TNF-α、IL-6、IL-10、IL-2和TGF-β1表达量显著高于对照组(P0.05),说明电离辐射所致的放射性损伤对相关细胞因子表达有影响,研究结果可为放射性损伤的诊断提供辅助参考。
[Abstract]:Radiotherapy has become an important method in the treatment of tumors, but at the same time, high doses of ionizing radiation will cause damage to various tissues and organs of the body. To investigate the effects of high dose ionizing radiation on pathological injury and expression of cytokines in the heart, liver, spleen, lung, kidney and intestine of rhesus monkey Macaca mulatta. In this study, three male rhesus monkeys were irradiated with 9.5Gy of 60Co 纬 for 4 hours. The animals were killed 15 days after irradiation and the tissues of related organs were collected. The pathological changes of heart, liver, spleen, lung, kidney and intestine were observed by hematoxylin-eosin staining with hematoxylin eosin (HE). The expression of tumor necrosis factor- 伪 (TNF- 伪), interleukin-6 (IL-6), interleukin-6 (IL-6) and transforming growth factor 尾 1(transforming growth factor 尾 1 (TGF- 尾 1) were detected by immunohistochemistry and image analysis. The results showed that the pathological changes of heart, liver, spleen, lung and kidney of rhesus monkey were observed 15 days after radiation. Moreover, the expressions of IL-2 and TGF- 尾 _ 1 in TNF- 伪 / IL-6 / IL-10 and TGF- 尾 _ 1 were significantly higher than those in the control group (P < 0.05), which indicated that the radiation damage induced by ionizing radiation had an effect on the expression of cytokines. The results of the study could provide an auxiliary reference for the diagnosis of radiation damage.
【作者单位】：四川农业大学动物医学院/实验动物疾病模型研究室;四川农业大学实验动物工程技术中心;四川普莱美生物科技集团有限公司;
【基金】：国家科技支撑计划(No.2014BAI03B01) 国家重大科学仪器设备开发专项(No.2013YQ49085906)
【分类号】：R730.55

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