中国西部森林昆虫病原真菌多样性与致病力研究
发布时间：2021-07-26 20:30
本研究探讨了我国西部森林中昆虫病原真菌（EFs）的分布与致病力。本研究由两部分组成:第一部分是确定秦岭佛坪自然保护区（FNNR）内昆虫病原真菌;第二部分是四川森林巴山与川西森林中的昆虫病原真菌。从佛坪自然保护区分离到绿僵菌,白僵菌等昆虫病原真菌,使用核基因的DNA序列数据和形态学特征鉴定种类并分析系统发育关系。使用MzIGS3基因片段分析佛坪自然保护区EFs的进化关系,通过PARB进化树对绿僵菌Metarhizium进行分析可以明确鉴定到四个不同的种及其进化关系;但是5’-TEF基因片段仅能够对绿僵菌种类进行有限而模糊的区分。白僵菌则是通过对翻译延伸因子EF-1α和Bloc区域的序列分析来进行鉴定和分析。绿僵菌物种的丰富度随着海拔的升高而降低,其中绿僵菌（Metarhizium robertsii s.l.）是沿海拔梯度最丰富的物种。来自四川省的EFs的形态学和分子特征证实了昆虫真菌病原体的四个主要属,即绿僵菌Metarhizium（173株）,白僵菌Beauveria（15株）,棒束孢属Isaria（10株）,淡紫紫霉属Purpureocillium（1株）和一...
【文章来源】：西北农林科技大学陕西省 211工程院校 985工程院校 教育部直属院校
【文章页数】：119 页
【学位级别】：博士
【文章目录】：
ABSTRACT
摘要
附图
CHAPTER ONE: INTRODUCTION
1.1 General view
1.2 Necessity of study and destructive impacts of biological pesticides
1.3 Natural occurrence of the entomopathogenic fungi species in nature
1.4 Cryptic species in fungal entomopathogens and the use of molecular delimitation forecological studies
1.5 Are fungi relatively free from dispersal barriers?
1.6 The applicability of the beneficial symbioant to fungal entomopathogens
1.7 Entomopathogenic fungi as a biological control agent
1.8 Thesis scope
CHAPTER TWO: GENERAL METHODS
2.1 Study site and soil sampling
2.2 Isolation of insect pathogenic fungi (Metarhizium and Beauveria) from FNNR soilsamples by an in vitro semi-differential medium method
2.3 Fungal isolation from Sichuan province soil samples with insect susceptible bait method
2.4 Morphology of 20 Metarhizium isolates from FNNR
2.5 Genomic DNA extraction, PCR amplification and sequencing:
2.6 Phylogenetic analysis:
2.7 Bioassay experiment:
2.7.1 Experimental insects
2.7.2 Preparation of conidia
2.8 Cold activity on conidial germination
2.9 Deposit of fungal strain in public service collection
2.10 Statistical analysis
2.11 Mantel test and species richness
CHAPTER THREE: RESULTS
3.1 RESULTS OF EFs FROM FNNR
3.1.1 Metarhizium occurrence and DNA analysis
3.1.2 Morphology of isolates and culture studies
3.1.3 Growth assessment and in vitro conidiation study
3.1.4 Virulence
3.1.5 Pathogenicity against L. dispar
3.1.6 Cold activity
3.2 Results from Sichuan Province
3.2.1 Occurrence and distribution of the entomopathogenic fungi
3.2.2 Phylogenetic analysis of fungal isolates
CAHPTER FOUR: DISCUSSION AND CONCLUSION
4.1 Discussion
4.2 Conclusion
CHAPTER FIVE: SUPPLEMENTARY DATA
References
LIST OF FIGURES
LIST OF TABLES
List of Abbreviations
Aknowledgement
Personal introduction
List of Publications
本文编号：3304315
【文章来源】：西北农林科技大学陕西省 211工程院校 985工程院校 教育部直属院校
【文章页数】：119 页
【学位级别】：博士
【文章目录】：
ABSTRACT
摘要
附图
CHAPTER ONE: INTRODUCTION
1.1 General view
1.2 Necessity of study and destructive impacts of biological pesticides
1.3 Natural occurrence of the entomopathogenic fungi species in nature
1.4 Cryptic species in fungal entomopathogens and the use of molecular delimitation forecological studies
1.5 Are fungi relatively free from dispersal barriers?
1.6 The applicability of the beneficial symbioant to fungal entomopathogens
1.7 Entomopathogenic fungi as a biological control agent
1.8 Thesis scope
CHAPTER TWO: GENERAL METHODS
2.1 Study site and soil sampling
2.2 Isolation of insect pathogenic fungi (Metarhizium and Beauveria) from FNNR soilsamples by an in vitro semi-differential medium method
2.3 Fungal isolation from Sichuan province soil samples with insect susceptible bait method
2.4 Morphology of 20 Metarhizium isolates from FNNR
2.5 Genomic DNA extraction, PCR amplification and sequencing:
2.6 Phylogenetic analysis:
2.7 Bioassay experiment:
2.7.1 Experimental insects
2.7.2 Preparation of conidia
2.8 Cold activity on conidial germination
2.9 Deposit of fungal strain in public service collection
2.10 Statistical analysis
2.11 Mantel test and species richness
CHAPTER THREE: RESULTS
3.1 RESULTS OF EFs FROM FNNR
3.1.1 Metarhizium occurrence and DNA analysis
3.1.2 Morphology of isolates and culture studies
3.1.3 Growth assessment and in vitro conidiation study
3.1.4 Virulence
3.1.5 Pathogenicity against L. dispar
3.1.6 Cold activity
3.2 Results from Sichuan Province
3.2.1 Occurrence and distribution of the entomopathogenic fungi
3.2.2 Phylogenetic analysis of fungal isolates
CAHPTER FOUR: DISCUSSION AND CONCLUSION
4.1 Discussion
4.2 Conclusion
CHAPTER FIVE: SUPPLEMENTARY DATA
References
LIST OF FIGURES
LIST OF TABLES
List of Abbreviations
Aknowledgement
Personal introduction
List of Publications
本文编号：3304315
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