New Allele of HL6 Regulates Trichome Elongation in Rice

发布时间：2021-03-15 14:23

　　Trichomes are specialized epidermal cells that play multiple roles in plant development. However, knowledge about the molecular mechanism of trichome development in rice is limited. In this study, a hairy leaf locus HL6^SWWR of rice variety Suwangwanger（SWWR） was identified by map-based cloning. Transgenic complementation and knock-out mutation demonstrated that HL6^SWWR regulated trichomes on leaves and expression levels of HL6^SWWR affected trichome elongation. Tr... 

【文章来源】：Rice Science. 2020,27(06)

【文章页数】：13 页

【文章目录】：
MATERIALS AND METHODS
    Plant materials
    Phenotype survey
    Linkage analysis and genetic mapping
    q RT-PCR analysis
    Plasmid construction and transformation
    Subcellular localization of HL6
    Transcriptional activation assay
    RNA-sequencing （RNA-Seq） analysis
RESULTS
    Morphological characteristics of epidermal trichome
    Genetic analysis of hairy leaf trait
    Map-based cloning of hairy leaf gene
    HL6SWWR regulates trichome elongation
    Natural variation in HL6
    Subcellular localization and transcriptional activation assay of HL6
    RNA-Seq analysis of plants with altered HL6 levels
DISCUSSION
SUPPLEMENTAL DATA



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