The involvement of wheat U-box E3 ubiquitin ligase Ta PUB1 i

发布时间：2021-04-10 15:52

　　U-box E3 ubiquitin ligases play important roles in the ubiquitin/26 S proteasome machinery and in abiotic stress responses. Ta PUB1-overexpressing wheat（Triticum aestivum L.） were generated to evaluate its function in salt tolerance. These plants had more salt stress tolerance during seedling and flowering stages, whereas the Ta PUB1-RNA interference（RNAi）-mediated knock-down transgenic wheat showed more salt stress sensitivity than the wild type（WT）. Ta PUB1 overexpression upregulated the expre... 

【文章来源】：Journal of Integrative Plant Biology. 2020,62(05)SCICSCD

【文章页数】：21 页

【文章目录】：
INTRODUCTION
RESULTS
    Generation and identification of TaPUB1‐overexpressing and RNAi‐mediated knock‐down wheat plants
    Salt stress tolerance of transgenic wheat plants during seedling and flowering stages
    The effects of TaPUB1 on the accumulation and transmembrane transport of K+and Na+in roots
    Effects of TaPUB1 on the ROS scavenging system of transgenic wheat plants under salt stress
    TaPUB1 modulates the stress‐responsive associated transcriptional regulatory network
    TaPUB1 interacts with TaMP in yeast and in plants
    Salt sensitivity of the TaMP‐overexpressing transgenic Brachypodium distachyon plants during seedling stage
DISCUSSION
    TaPUB1 positively regulates salt tolerance of wheat
    Accumulation and transportation of Na+/K+ions is an important mechanism of TaPUB1 action in theregulation of salt tolerance
    TaPUB1 widely modulates the transcriptional levels of salt stress‐associated genes
    Interaction of TaPUB1 with TaMP regulates the salt tolerance of wheat
MATERIALS AND METHODS
    Plant material,growth conditions and salt stress treatments
    Plant transformation
    Detection of transgenic plants
    Chlorophyll content and photosynthesis parameter assays
    Measurements of net Na+,K+,and H+flux in plant roots
    Measurement of ROS parameters
    Measurement of antioxidant enzyme activities
    Screening for TaPUB1‐interacting proteins by Y2H assay
    Verifying the interaction of TaPUB1 and TaMP by BiFC assay
    Statistical analysis
AUTHOR CONTRIBUTIONS
SUPPORTING INFORMATION
    Figure S1.The expression level of TaPUB1 in different concentrations of salt
    Figure S2.Sequence analysis of TaMP isolated from Chinese spring wheat
    Figure S3.Molecular analysis of TaMP transgenic Brachypodium distachyon
    Figure S4.The confirmation of the genotype of atgh38mutant



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