Maize WI5 encodes an endo-1,4-β-xylanase required for second

发布时间：2021-06-16 10:46

　　Water transport from roots to leaves through xylem is important for plant growth and development.Defects in water transport can cause drought stress, even when there is adequate water in the soil. Here, we identified the maize（Zea mays） wilty5（wi5） mutant, which exhibits marked dwarfing and leaf wilting throughout most of its life cycle under normal growth conditions. wilty5 seedlings exhibited lower xylem conductivity and wilted more rapidly under drought, NaC l, and high temperature treatments... 

【文章来源】：Journal of Integrative Plant Biology. 2020,62(10)SCICSCD

【文章页数】：18 页

【文章目录】：
INTRODUCTION
RESULTS
    Identification of the drought‐sensitive mutant wi5 in maize
    We crossed wi5 plants with B73 plants.All F1plants displayed a wild‐type phenotype.Progeny derived from backcrossing F1to wi5 plants showed an approximately 1:1 segregation ratio of wild‐type:wi5mutant phenotypes,suggesting that wi5 is a single‐gene recessive nuclear mutant (Table S1).
    wilted5 plants are hypersensitive to drought,high temperature,and Na Cl at the seedling stage
    wilted5 mutants exhibit a hypersensitive response to water stress,but impaired water transport system
    Map‐based cloning and complementation of the wi5mutant
    Tissue‐specific expression profiles of WI5
    WILTED5 encodes a GH10 family member
    Mutation in wi5 alters secondary cell wall structure and xylan deposition
DISCUSSION
    WILTED5 is a member of the GH10 family and is highly active in internodes undergoing secondary cell wall thickening
    Altered cell wall structure and composition lead to stunted growth in wi5
    Possible role of WI5 in regulating plant growth and transporting water
MATERIALS AND METHODS
    Plant materials and growth conditions
    Measurement of water loss of detached leaves
    Measurement of leaf ABA content
    Histochemical analysis
    Genetic analysis and marker development
    Copy number analysis and testing of a transgenic complementation event
    Sequence and phylogenetic analyses
    Xylanase activity assay
    RT‐PCR and real‐time PCR
    Transcriptome analysis
    Xylan immunolocalization
    Cell wall component analysis
    Injury flow test
AUTHOR CONTRIBUTIONS
SUPPORTING INFORMATION



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