甘蓝型油菜磷脂酶家族的全基因组分析

发布时间：2021-08-01 17:38

　　甘蓝型油菜是一种重要的油料作物,在世界范围内广泛种植。干旱,寒冷和盐等非生物胁迫,会影响其生长发育和产量。磷脂酶家族包括:磷脂酶A（PLAs）,磷脂酶C（PLCs）和磷脂酶D（PLDs）,它们在植物的生长和发育中起重要作用,并且可以增强植物对非生物胁迫的适应。在这项研究中,基于它们对磷脂的水解活性、N末端序列、序列相似性、亚细胞定位和底物偏好性,29个BnapPLA基因被鉴定并分为三个主要的亚组:pPLAI,pPLAII（α,β,γ）和pPLAIII（α,β,γ和δ）。这些基因亚家族之间存在一些差异,如蛋白质大小,基序及其在结构域中的数量,以及其他理化性质。尽管BnapPLAs与AtpPLAs中具有直系同源性存在高度相似性,而甘蓝型油菜中每个pPLA亚家族的基因成员约是拟南芥中的3倍。基因表达分析表明,BnapPLAIIIs在大多数组织中大量表达,而BnapPLAIIs的表达在植物组织中表现出一定的特异性。BnapPLAIIIβ,BnapPLAIIIα和BnapPLAICnn均受到不同的非生物胁迫诱导,如缺水,NaCl,ABA和4℃低温,BnapPLAIIIδ则对ABA胁迫有明显的响应... 

【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校

【文章页数】：134 页

【学位级别】：博士

【文章目录】：
摘要
Abstract
List of Abbreviations
CHAPTER Ⅰ.LITERATURE REVIEW
    1.1 Introduction
    1.2 pPLA:Patatin-related pPLAs regulated metabolism,growth and development inplants
        1.2.1 pPLA classification and domain structural information
        1.2.2 Substrate preferences of pPLA
        1.2.3 Subcellular localization
        1.2.4 Cellular and physiological roles
    1.3 PLC:NPC and PI-PLC which produce DAG as signal transducer in plants
        1.3.1 NPC:roles in hydrolysis of membrane phospholipids
        1.3.2 PI-PLC:phosphoinositide-hydrolyzing PLCs which produce IP3 or IP
    1.4 PLD:PLD and PX/PH-PLD subfamilies which produce PA as signalingmolecule under stresses
        1.4.1 Classification and domain structural analysis
        1.4.2 Catalytic properties of PLDs and their activity regulation
        1.4.3 Tissue distribution and subcellular location
        1.4.4 PLDs physiological and cellular roles
        1.4.5 Cellular functions regulated by PLDs
        1.4.6 Phosphatidic acid as lipid mediators
    1.5 Objectives
CHAPTER Ⅱ.MATERIALS AND METHODS
    2.1 Characterization of phospholipase genes in Brassica napus
        2.1.1 Phospholipase genes identification in Brassica napus
        2.1.2 Phylogenetic analysis of Brassica napus phospholipase genes
        2.1.3 Analysis of gene structure and conserved domains and motifs in Brassicanapus
    2.2 Plant material,growth condition and stress treatments
    2.3 RNA extraction
    2.4 Differential expression and expression under stress treatments
    2.5 Transgene constructs and plant transformation
    2.6 Protein extraction,immunoblotting,and activity assays
    2.7 Lipid extraction and analysis
    2.8 Fatty acid composition and oil content
    2.9 Agronomical traits analysis
CHAPTER Ⅲ.RESULTS
    3.1 Phospholipase A family in Brassica napus
        3.1.1 Characterization of BnapPLAs
        3.1.2 Gene expression analysis
        3.1.3 Lysolipids analysis under abiotic stresses
    3.2 Phospholipase C family in Brassica napus
        3.2.1 Characterization of BnaPLCs
        3.2.2 Expression analysis of BnaPLC genes
        3.2.3 Analysis of DAG under abiotic stresses
    3.3 Phospholipase D family in Brassica napus
        3.3.1 Characterization of PLD in Brassica napus
        3.3.2 Expression analysis of BnaPLDs genes
        3.2.3 Lipidomic analysis
        3.3.4 Functional characterization of PLDα1 in Brassica napus
CHAPTER Ⅳ.DISCUSSION
    4.1 Characterization of BnapPLA genes
        4.1.1 Bioinformatics based characterization
        4.1.2 Gene expression analysis and lipid remodeling under abiotic stresses
    4.2 Characterization of BnaPLC genes
        4.2.1 Bioinformatics based characterization
        4.2.2 Expression analysis and lipid remodeling by BnaPLCs under abioticstresses
    4.3 Characterizations of BnaPLD genes
        4.3.1 Bioinformatics based characterization of BnaPLD
        4.3.2 Expression analysis and lipid remodeling under abiotic stresses
        4.3.3 Functional characterization of PLDα1 in Brassica napus
CHAPTER Ⅴ.CONCLUSIONS
    5.1 Conclusion
APPENDIX
REFERENCES
PUBLICATIONS
ACKNOWLEDGEMENTS


【参考文献】：
期刊论文
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[2]NIRS定量分析油菜种子含油量、蛋白质含量数学模型的创建[J]. 甘莉,孙秀丽,金良,王高全,徐久伟,魏泽兰,傅廷栋.  中国农业科学. 2003(12)



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