Transcriptome and metabolome profiling of unheading in F 1 h

发布时间：2021-08-12 15:32

　　Heading date is a crucial agronomic trait. However, rice usually delays heading due to the photoperiod, temperature, hormones or age. The present research was conducted to analyze the mechanism controlling heading date in F₁ hybrid rice. We constructed two test-crossing populations using two introgression lines（ILs）, P20 and P21 coming from SH527/FH838 as the male parent, respectively, and male sterile line Jin23 A as the female parent. Meanwhile, the F₁ hybrids of H20, obt... 

【文章来源】：Journal of Integrative Agriculture. 2020,19(10)SCICSCD

【文章页数】：16 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Plant materials
    2.2. Planting methods and sampling strategies
    2.3. RNA isolation,library construction and RNA-Seq analysis
    2.4. Metabolite extraction and profiling analysis
3. Results
    3.1. Heading date of F1 hybrids in response to photoperiodic regulation
    3.2. Global analysis of RNA-Seq date
    3.3. The F1 hybrid,H20,was sensitive to photoperiod which acted on the input signal for circadian rhythm
    3.4. Oxidative reduction was probably the key factor causing the difference in heading in H20-LDcompared with H21-LD,P20-LD and H20-SD
    3.5. FT and oxidoreductase-like gene displayed co-regulation in both H20-SD vs.H20-LD and H21-LD vs.H20-LD at periods II and III
    3.6. Both endogenous (genetic) and exogenous(environmental) regulation function in no heading in the F1 hybrids
    3.7. The pathway of DHD1/Os MFT1-Ehd1-RFT1-Os MADS14/Os MADS18 was the foundation for no heading in the F1 hybrids
    3.8. The genes related to the GSH/GSSG cycle also regulated heading with oxidation reduction processes
    3.9. More DMs were detected between H20-SD and H20-LD,but only a few DMs between H21-LD and H20-LD,which was consistent with transcription data
    3.1 0. RT-q PCR analysis
4. Discussion
    4.1. The circadian rhythm pathway plays a vital role for unheading in H20 under LD condition
    4.2. The active oxidative reduction process occurs in H20 under LD condition
    4.3. The synergistic effect of the circadian rhythm pathway and glutathione metabolism leads to unheading in H20 under LD condition
5. Conclusion


【参考文献】：
期刊论文
[1]Dissection of Genetic Mechanism of Abnormal Heading in Hybrid Rice[J]. ZHANG Hong-jun,QU Li-jun,XIANG Chao,WANG Hui,XIA Jia-fa,LI Ze-fu,GAO Yong-ming,SHI Ying-yao.  Rice Science. 2014(04)
[2]杂交稻“大青棵”现象遗传基础剖析[J]. 曲丽君,张宏军,项超,王辉,夏加发,李泽福,高用明,石英尧.  中国水稻科学. 2013(06)



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