Investigation of CRISPR/Cas9-induced SD1 rice mutants highli

发布时间：2021-10-10 04:09

　　Although Clustered Regularly Interspaced Short Palindromic Repeats（CRISPR）/CRISPR-associated 9（Cas9） system has been widely used for basic research in model plants,its application for applied breeding in crops has faced strong regulatory obstacles,due mainly to a poor understanding of the authentic output of this system,particularly in higher generations.In this study,different from any previous studies,we investigated in detail the molecular characteristics and production performance of CRISPR/... 

【文章来源】：Journal of Genetics and Genomics. 2020,47(05)SCICSCD

【文章页数】：8 页

【文章目录】：
1. Introduction
2. Results
    2.1. SD1-edited semidwarf T2plants harbor large chromosomal deletion and insertion
    2.2. SD1-edited semidwarf T2plants display diverse genotypes
    2.3. Some SD1-edited semidwarf T2plants display possible off-target mutation
    2.4. Most of SD1-edited semidwarf T2plants still harbor exogenous elements
    2.5. Genotypes of edited SD1 in semidwarf T2plants pass over stably in a Mendel way
    2.6. Exogenous elements in semidwarf T2plants segregate irregularly
    2.7. CRISPR/Cas9-edited SD1 alleles reduce plant height significantly but affect yield differently
3. Discussion
    3.1. Signatures
    3.2. Off-targets
    3.3. Exogenous elements
    3.4. From laboratory to field
4. Materials and methods
    4.1. Plasmid vector construction
    4.2. Plant materials and growth conditions
    4.3. Plant genomic DNA extraction
    4.4. Genotype and exogenous elements analysis
    4.5. Plant height and yield measurement


【参考文献】：
期刊论文
[1]Engineering guide RNA to reduce the off-target effects of CRISPR[J]. Jing Wu,Hao Yin.  Journal of Genetics and Genomics. 2019(11)



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