Differential Expression of Rice Valine-Qlutamine Gene Family

发布时间：2021-10-10 18:47

　　The functional diversity of plant valine-qlutamine（VQ） proteins is closely associated with their partners WRKY transcription factors, and also with a complex network of signaling pathways that mediated by hormone molecules. We reported genome-wide expression profiles of differentially expressed rice VQ genes under nitric oxide（NO） treatment based on a microarray analysis. Cluster analysis of expression patterns revealed that some VQ genes and WRKY genes shared similar expression trends. Predicti... 

【文章来源】：Rice Science. 2020,27(01)SCICSCD

【文章页数】：11 页

【文章目录】：
MATERIALS AND METHODS
    Plant materials and NO treatment
    RNA isolation,microarray hybridization and data process
    Quantitative real-time PCR(qRT-PCR)analysis
    Analysis for cis-elements of promoters
    Transient expression assay in tobacco cells
    Chromatin immunoprecipitation(ChIP)assay
    Yeast two-hybrid assay
RESULTS
    Some VQ genes were differentially expressed under NO treatment
    W-box,or W-box like cis-elements were enriched in promoters of differentially expressed VQ genes
    Some VQ genes showed similar expression patterns with WRKY genes in response to NO treatment
    OsWRKY24 binded to promoters of Os VQ7 and Os WRKY24 itself
    OsVQ7 interacted physically with OsWRKY24through C-terminal WRKY domain
DISCUSSION
    VQ genes may be involved in NO signalingmediated physiological processes
    Similar expression of WRKY and VQ genes suggests their possible interactions during regulatory processes
    Could OsVQ7 and OsWRKY24 module play a role in regulating plant immunity?
SUPPLEMENTAL DATA


【参考文献】：
期刊论文
[1]响应镉胁迫的水稻WRKY15转录因子基因的分离与表达特征[J]. 彭喜旭,白宁宁,王海华.  中国水稻科学. 2018(02)
[2]水稻WRKY转录因子基因家族响应外源一氧化氮的表达谱分析[J]. 孟姣,王海华,向建华,蒋丹,彭喜旭,贺欢欢.  中国水稻科学. 2016(02)
[3]拟南芥VQ基因家族响应抗性相关激素表达谱分析[J]. 林渊源,余迪求.  植物分类与资源学报. 2012(05)



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