GDSL esterase/lipases OsGELP34 and OsGELP110/OsGELP115 are e

发布时间：2021-10-15 06:00

　　Pollen exine contains complex biopolymers of aliphatic lipids and phenolics. Abnormal development of pollen exine often leads to plant sterility. Molecular mechanisms regulating exine formation have been studied extensively but remain ambiguous. Here we report the analyses of three GDSL esterase/lipase protein genes,OsGELP34, OsG ELP110, and Os GELP115, for rice exine formation. OsGELP34 was identified by cloning of a male sterile mutant gene. OsG ELP34 encodes an endoplasmic reticulum protein a... 

【文章来源】：Journal of Integrative Plant Biology. 2020,62(10)SCICSCD

【文章页数】：20 页

【文章目录】：
INTRODUCTIONO
RESULTS
    Isolation and genetic analysis of the male sterile mutant osgelp34
    Cloning of the mutant gene
    The mutant gene encodes a putative GDSL lipase/esterase
    Os GELP34 is mainly expressed in anthers during meiosis
    Microscopic analysis of the mutant anther development
    Mutation of Os GELP34 affected the expression of multiple genes acting in the tapetal PCD and pollen exine formation
    Os GELP110 and Os GELP115 are redundant but essential for pollen development
DISCUSSION
MATERIALS AND METHODS
    Plant materials and growth conditions
    Characterization of the mutant phenotype
    Microscopy and histology
    Molecular cloning of Os GELP34 and HRM analysis
    Plasmid construction,rice transformation,and transgene determination
    Gene expression analysis by q RT‐PCR
    GUS staining
    Protein subcellular localization assay
    Computational analysis of the GELP proteins
AUTHOR CONTRIBUTIONS
SUPPORTING INFORMATION



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