OsABA8ox2, an ABA catabolic gene, suppresses root elongation
发布时间：2021-10-19 21:03
In rice, OsABA8ox encodes abscisic acid（ABA） 8′-hydroxylase, which catalyzes the committed step of ABA catabolism. The contribution of ABA catabolism in root development remains unclear. We investigated the role of OsABA8ox2 in root growth and development and drought response. GUS staining results showed that OsABA8ox2 was expressed mainly in roots at seedling stage and was strongly expressed in the meristematic zone of the radicle. OsABA8ox2 expression in roots was markedly decreased after 0.5 ...
【文章来源】：The Crop Journal. 2020,8(03)SCICSCD
【文章页数】：12 页
【文章目录】：
1. Introduction
2. Materials and methods
2.1. Plant materials
2.2. Vector construction and rice transformation
2.3. GUS staining
2.4. RT-PCR and quantitative RT-PCR(qPCR)
2.5. Plant growth and drought stress treatment
2.6. Measurement of ABA and IAA contents
2.7. Measurement of photosynthetic indices
2.8. Measurement of malondialdehyde(MDA)content
2.9. Subcellular localization
2.1 0. Statistical analysis
3. Results
3.1. Rice OsABA8ox2 is a drought-responsive gene in roots
3.2. OsABA8ox2 knockout plants show increased rice drought tolerance
3.3. OsABA8ox2 KO plants show increased ABA and IAA accumulation in roots under drought stress
3.4. OsABA8ox2 overexpression suppresses root elongation of rice seedlings,while KO seedlings develop more vertically oriented RSA beneficial to drought tolerance
3.5. OsABA8ox2 overexpression increases stomatal conduc-tance and transpiration rate
3.6. OsABA8ox2 knockout retards post-germination growth and increases ABA sensitivity
3.7. OsABA8ox2 and OsABA8ox3 are both localized in endoplasmic reticulum(ER)
4. Discussion
4.1. Expression specificity of OsABA8ox2
4.2. OsABA8ox2 contributes to drought-induced ABA and IAA accumulation in roots
4.3. OsABA8ox2 shows sophisticated modulation on root growth and root architecture
4.4. Excessive transpirational water loss could partially account for the severe wilting phenotype of OsABA8ox2 OEseedlings under drought stress
4.5. Conclusion and perspective
本文编号：3445597
【文章来源】：The Crop Journal. 2020,8(03)SCICSCD
【文章页数】：12 页
【文章目录】：
1. Introduction
2. Materials and methods
2.1. Plant materials
2.2. Vector construction and rice transformation
2.3. GUS staining
2.4. RT-PCR and quantitative RT-PCR(qPCR)
2.5. Plant growth and drought stress treatment
2.6. Measurement of ABA and IAA contents
2.7. Measurement of photosynthetic indices
2.8. Measurement of malondialdehyde(MDA)content
2.9. Subcellular localization
2.1 0. Statistical analysis
3. Results
3.1. Rice OsABA8ox2 is a drought-responsive gene in roots
3.2. OsABA8ox2 knockout plants show increased rice drought tolerance
3.3. OsABA8ox2 KO plants show increased ABA and IAA accumulation in roots under drought stress
3.4. OsABA8ox2 overexpression suppresses root elongation of rice seedlings,while KO seedlings develop more vertically oriented RSA beneficial to drought tolerance
3.5. OsABA8ox2 overexpression increases stomatal conduc-tance and transpiration rate
3.6. OsABA8ox2 knockout retards post-germination growth and increases ABA sensitivity
3.7. OsABA8ox2 and OsABA8ox3 are both localized in endoplasmic reticulum(ER)
4. Discussion
4.1. Expression specificity of OsABA8ox2
4.2. OsABA8ox2 contributes to drought-induced ABA and IAA accumulation in roots
4.3. OsABA8ox2 shows sophisticated modulation on root growth and root architecture
4.4. Excessive transpirational water loss could partially account for the severe wilting phenotype of OsABA8ox2 OEseedlings under drought stress
4.5. Conclusion and perspective
本文编号：3445597
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