Identification and gene mapping of the starch accumulation a

发布时间：2021-10-29 13:04

　　The rice mutant ossac4（starch accumulating 4） was raised from seeds of the rice（Oryza sativa L.） indica maintainer line Xinong 1 B treated with ethyl methanesulfonate. The distal and medial portions of the second leaf displayed premature senescence in the ossac4 mutant at the four-leaf stage. Physiological and biochemical analysis, and cytological examination revealed that the ossac4 mutant exhibited the premature leaf senescence phenotype. At the four-leaf stage, the leaves of the ossac4 mutant... 

【文章来源】：Journal of Integrative Agriculture. 2020,19(09)SCICSCD

【文章页数】：15 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Plant materials
    2.2. Pigment concentration and photosynthetic parameter measurement
    2.3. Lipid peroxidation and ROS-scavenging enzyme assays
    2.4. Cytological analysis
    2.5. Determination of sugar concentration and iodine-potassium iodide staining in leaves
    2.6. Extraction of total RNA and quantitative real-time PCR analysis
    2.7. Genomic DNA extraction and PCR amplification
    2.8. Genetic analysis and genetic map construction
3. Results
    3.1. Phenotypes and agronomic traits
    3.2. Chloroplast and photosynthetic characteristics
    3.3. Physiological parameters
    3.4. Leaf sugar content analysis
    3.5. Expression of genes related to glucose and photosynthetic metabolism
    3.6. Genetic analysis and genetic map construction
4. Discussion
    4.1. Mutation of Os SAC4 induces leaf starch accumulation
    4.2. Mutation of Os SAC4 severely inhibits chloroplast functioning
    4.3. Mutation of Os SAC4 induces premature leaf senescence
5. Conclusion


【参考文献】：
期刊论文
[1]水稻叶片淀粉积累及早衰突变体esl9的鉴定与基因定位[J]. 肖艳华,陈新龙,杜丹,邢亚迪,张天泉,祝毛迪,刘明明,朱小燕,桑贤春,何光华.  作物学报. 2017(04)



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