Integrated transcriptome, small RNA, and degradome analysis

发布时间：2021-11-19 17:52

　　The mesocotyl, a structure located between the basal part of the seminal root and the coleoptile node of seedlings, contributes to pushing the shoot tip through the soil surface, a function that is essential for the uniform emergence of direct-seeded rice. Its elongation is inhibited by light and induced in darkness. This investigation of an indica rice（P25） with vigorous mesocotyl elongation was aimed at identifying the "omics" basis of its lightinduced growth inhibition. A transcriptomic compa... 

【文章来源】：The Crop Journal. 2020,8(06)SCICSCD

【文章页数】：11 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Plant materials,growing conditions,and assessment of phenotype
    2.2. Transcriptome library construction,sequencing,and analysis
    2.3. Small RNA library construction,sequencing,and analysis
    2.4. Degradome sequencing,target identification and analysis
    2.5. Validation of differential abundance by quantitative real-time PCR
    2.6. Validation of mi RNA/target interactions
    2.7. Measurement of tissue indole-3-acetic acid (IAA) content and PAO activity
3. Results
    3.1. Phenotypic variation in mesocotyl length
    3.2. The mesocotyl transcriptome from darkness to light exposure
    3.3. The set of mi RNAs responding to light exposure
    3.4. Target prediction of mi RNAs derived via degradome sequencing
    3.5. A co-expression regulatory network based on mi RNAs,targets,and DEGs
    3.6. Antagonistic regulation of mesocotyl elongation by auxin and light
4. Discussion
    4.1. Dynamic transcriptome profiling sheds light on the complex regulation network of mesocotyl elongation
    4.2. Post-transcriptional regulation network of mi RNAs that responds to light exposure in the rice seedling mesocotyl
    4.3. PAO activity of mesocotyl is a key indicator during light exposure
5. Conclusions



本文编号：3505552