Natural variation in the promoter of OsHMA 3 contributes to

发布时间：2022-01-09 08:28

　　Rice is a major source of cadmium（Cd） intake for Asian people. Indica rice usually accumulates more Cd in shoots and grains than Japonica rice. However, underlying genetic bases for differential Cd accumulation between Indica and Japonica rice are still unknown. In this study, we cloned a quantitative trait locus（QTL） grain Cd concentration on chromosome 7（GCC7） responsible for differential grain Cd accumulation between two rice varieties by performing QTL analysis and map-based cloning. We foun... 

【文章来源】：Journal of Integrative Plant Biology. 2020,62(03)SCICSCD

【文章页数】：16 页

【文章目录】：
INTRODUCTION
RESULTS
    Identification of QTLs for grain Cd accumulation in a RIL population
    Physiological characterization of the QTL GCC7
    Map-based cloning of GCC7
    Sequence variation in OsHMA3 promoter is responsible for the differential Cd accumulation
    Genome-wide sequence analysis of OsHMA3 promoter and the application of GCC7PA64sto reduce Cd accumulation in Indica rice
DISCUSSION
MATERIALS AND METHODS
    Plant materials and field and pot experiments
    Hydroponic experiments
    Determination of Cd content in plant tissues
    QTL analysis and fine mapping of GCC7
    Plasmid construction and rice transformation
    Expression analysis
    Promoter activity assay in rice protoplasts
    Distribution analysis of GCC7 allele in rice accessions
SUPPORTING INFORMATION


【参考文献】：
期刊论文
[1]Reduction in cadmium accumulation in japonica rice grains by CRISPR/Cas9-mediated editing of OsNRAMP5[J]. YANG Chang-hong,ZHANG Yang,HUANG Chao-feng.  Journal of Integrative Agriculture. 2019(03)



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