OsSRK1, an Atypical S-Receptor-Like Kinase Positively Regula

发布时间：2022-08-02 10:27

　　Receptor-like kinases（RLKs） are important for plant growth, development and defense responses. The S-receptor protein kinases（SRKs）, which represent an RLK subfamily, control the selfincompatibility among Brassica species. However, little information is available regarding SRK functions in rice. We identified a gene OsSRK1 encoding an atypical SRK. The transcript of OsSRK1 was induced by abscisic acid（ABA）, salt and polyethylene glycol. OsSRK1 localized to the plasma membrane and cytoplasm. Leaf... 

【文章页数】：10 页

【文章目录】：
MATERIALS AND METHODS
    Rice materials and growth conditions
    Histological analysis
    Reverse transcription polymerase chain reaction(RT-PCR)
    Quantitative RT-PCR(qRT-PCR)
    Construction of plant expression vector and rice transformation
    Production of OsSRK1-green fluorescent protein(GFP)fusion protein
    GeneChip analysis
RESULTS
    OsSRK1 is an atypical S-receptor-like kinase localized to plasma membrane and cytoplasm
    Expression patterns of OsSRK1
    Overexpression of OsSRK1 increased leaf width
    Overexpression of Os SRK1 enhanced ABA sensitivity and salt tolerance
    Genes regulated by OsSRK1
DISCUSSION
    Os SRK1 is not a typical S-receptor-like kinase
    Overexpression of OsSRK1 increases leaf width probably by promoting cell cycle in leaf primordium
    Overexpression of Os SRK1 enhances salt tolerance probably by regulating stress-related genes andABA pathway
SUPPLEMENTAL DATA


【参考文献】：
期刊论文
[1]水稻蛋白激酶基因启动子OsSRLp的分离及特性分析[J]. 王庆国,王莹莹,李臻,潘教文,王一帆,李颖秀,刘炜.  中国水稻科学. 2018(04)



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