Targeted mutagenesis of amino acid transporter genes for ric

发布时间：2022-11-12 13:22

　　High grain protein content（GPC） reduces rice eating and cooking quality（ECQ）. We generated OsAAP6 and OsAAP10 knockout mutants in three high-yielding japonica varieties and one japonica line using the CRISPR/Cas9 system. Mutation efficiency varied with genetic background in the T₀ generation, and GPC in the T₁ generation decreased significantly,owing mainly to a reduction in glutelin content. Amylose content was down-regulated significantly in some Osaap6 and all Osaap10 mu... 

【文章页数】：8 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Field planting and trait measurement
    2.2. Vector construction and transformation
    2.3. Grain protein content(GPC)measurement
    2.4. Amino acid determination
    2.5. Measurement of pasting properties
    2.6. Taste value measurement
    2.7. RNA extraction and qRT-PCR
    2.8. Statistical analysis
3. Results
    3.1. Generation of OsAAP6 and OsAAP10 mutations
    3.2. Grain protein content(GPC)of the OsAAP6 and OsAAP10mutants
    3.3. Amino acid content in Osaap6 and Osaap10 mutants
    3.4. Starch content and physicochemical properties
    3.5. Taste quality in Osaap6 and Osaap10 mutants
    3.6. Agronomic trait in Osaap6 and Osaap10 mutants
4. Discussion
5. Conclusions
Declaration of competing interest
Appendix A.Supplementary data


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