DEK43 is a P-type pentatricopeptide repeat(PPR)protein respo

发布时间：2023-05-20 06:33

　　Mitochondria, the main energy transducers in plant cells, require the proper assembly of respiratory chain complexes Ⅰ–Ⅴ for their function. The NADH dehydrogenase4(nad4) gene encodes mitochondrial respiratory chain complex Ⅰ subunit Ⅳ, but the mechanism underlying nad4 transcript splicing is unclear. Here, we report that the P-type pentatricopeptide repeat(PPR) protein DEFECTIVE KERNEL 43(DEK43) is responsible for cis-splicing of the nad4 transcript in maize. We demonstrate that DEK43 localizes...

【文章页数】：15 页

【文章目录】：
INTRODUCTION
RESULTS
    The kernels of dek43‐1 mutant are defective
    The Dek43 gene was characterized by positional cloning
    Dek43 encodes a novel P‐type PPR protein with a dual subcellular localization
    Mutations in Dek43 affect cis‐splicing of mitochondrial nad4 introns 1 and 3
    The dek43‐1 mutant exhibits reduced mitochondrial complex I abundance and activity
    The dek43 mutant exhibits altered mitochondrial ultrastructure and activation of the alternative respiratory pathway
DISCUSSION
    Dek43 encodes a P‐type PPR protein that is dual‐localized to the mitochondria and nucleus in maize
    DEK43 is required for the splicing of mitochondrial nad4 introns 1 and 3 and for complex I biogenesis
    DEK43 supports mitochondrial function and maize kernel development
MATERIALS AND METHODS
    Plant materials
    Histological analysis
    Transmission electron microscopy
    Map‐based cloning
    RNA extraction and qRT‐PCR
    Immunoblot analysis
    Isolation and analysis of mitochondrial complexes
SUPPORTING INFORMATION



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