Generation of selectable marker-free soft transgenic rice wi

发布时间：2024-03-08 21:19

　　The amylose content(AC) of rice endosperm starch varies from 0 to 35%,and is associated with rice cooking and eating quality.Soft rice has low AC,generally between 6% and 15%,and its eating quality is high whether it is consumed hot or cold.However,the appearance quality of current soft rice cultivars needs to be improved,especially opaque endosperm.Conventional genetic engineering has improved some agronomic traits of soft rice varieties,but not endosperm appearance.In the present study,a RNAi ...

【文章页数】：9 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Plant materials
    2.2. Vector construction
    2.3. Rice transformation and selection of SMF transgenic plants
    2.4. PCR and quantitative reverse transcription PCR(qRT-PCR)analysis
    2.5. Flour preparation
    2.6. Measurement of physicochemical parameters and starch viscosity
    2.7. Agronomic trait evaluation and statistical analysis
3. Results
    3.1. Production of transformants and identification of co-transformed rice plants
    3.2. Breeding of SMF transgenic lines containing the SSSII-2RNAi construct
    3.3. Expression of the SSSII-2 gene in SMF transgenic plants
    3.4. Quality characteristics of transgenic rice kernel starch
    3.5. Agronomic traits and kernel characteristics of transgenic lines
4. Discussion



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