Effects of Salinity on Photosynthetic Traits, Ion Homeostasi

发布时间：2024-03-09 16:28

　　了解作物对盐胁迫的生理生化反应有助于提高作物生长性能,增加作物产量。在本研究中,我们研究了不同类型盐胁迫下野大豆和栽培大豆幼苗的生长、光合作用参数、离子浓度以及参与氮同化相关酶活性的变化。研究结果显示,两种类型盐胁迫均会对野大豆和栽培大豆的生长和代谢产生不利影响。生长和光合参数随盐浓度的增加而显著降低。此外,根部钠(Na⁺)和氯(Cl^-)浓度显著增加。然而,在中性盐和碱性盐胁迫下,野大豆光合性能以及离子调控能力显著高于栽培大豆.在野大豆和栽培大豆幼苗叶片中,硝酸还原酶(NR)活性和谷氨酰胺合成酶/谷氨酰胺氧谷氨酸氨基转移酶(GS/GOGAT)循环均显呈下降趋势。而胺化谷氨酸脱氢酶(NADH-GDH,谷氨酸脱氢酶(NAD⁺-GDH,丙氨酸氨基转移酶(AlaAT)以及天冬氨酸氨基转移酶(AspAT)的活性显著增加。总之,与野大豆相比,栽培大豆的生长,光合作用,离子调节和氮同化酶的下降幅度更大。栽培大豆对盐胁迫更加敏感,碱性盐胁迫的影响高于中性盐胁迫。野生大豆具有更好的光合作用调节机制和硝酸盐同化机制。本研究结果能够为野大豆种...

【文章页数】：63 页

【学位级别】：硕士

【文章目录】：
Abstract
摘要
Chapter1:Introduction
    1.1 An overview of Wild and Cultivate soybean
    1.2 Salinity stress
        1.2.1 Types of salinity stress
        1.2.2 Impacts of salinity stress
        1.2.3 Impacts of salinity stress on ions homeostasis
        1.2.4 Impacts of salinity stress on photosynthesis
        1.2.5 Impacts of salinity stress on nitrogen(N)metabolism
    1.3 Chitchat of nitrogen and carbon metabolism
    1.4 Mechanisms of Salinity Tolerance
        1.4.1 Salinity stress and ionic regulation
        1.4.2 Salinity stress and antioxidant defense system
        1.4.3 Salinity Stress and organic compatible solutes
    1.5 Review of Literature
    1.6 The purpose and significance of this study
        1.6.1 Scientific problem
        1.6.2 Aims and contents of the study
        1.6.3 Research theoretical value and practical significance
Chapter2:Materials & Methods
    2.1 Plant materials and salt treatments
    2.2 Growth Parameters
    2.3 Gas Exchange Parameters
    2.4 Measurement of Photosynthetic Pigments
    2.5 Mineral ion content determination
    2.6 Enzyme extracts preparation
        2.6.1 Nitrate reductase(NR)activity
        2.6.2 Glutamine synthetase(GS)activity
        2.6.3 Glutamine synthase(GOGAT)activity
        2.6.4 Glutamate dehydrogenase(NADH-and NAD-GDH)activity
        2.6.5 Aminotransferase(AspAT and AlaAT)activity
        2.6.6 Protein determinations
    2.7 Data Analysis
Chapter3:Results
    3.1 Changes in plant growth performance
    3.2 Analysis of the ionome
        3.2.1 Changes in cation concentrations
        3.2.2 Changes in anion concentrations
    3.3 Changes in photosynthesis parameters
        3.3.1 Changes in photosynthetic pigments
        3.3.2 Changes in photosynthetic gaseous exchange parameters
    3.4 Changes in nitrogen assimilation enzyme activities
        3.4.1 Changes in nitrate reductase(NR)activity
        3.4.2 Changes in glutamine synthetase/glutamine synthase(GS/GOGAT)activities
        3.4.3 Changes in glutamate dehydrogenase and aminotransferases activities
Chapter4:Discussions
Chapter5:Conclusions
Chapter6:References
List of Abbreviation
Acknowledgments



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