燕麦种质资源多样性及重要农艺性状的QTL分析
发布时间：2024-05-15 04:11
燕麦(Avena spp.)是营养丰富的粮食和重要的饲料来源。栽培燕麦有两种类型,即皮燕麦和裸燕麦。皮燕麦广泛分布于世界的温带地区,而裸燕麦主要在中国的华北、西北和西南地区种植。燕麦因具有预防皮肤病、高胆固醇症、心血管疾病和糖尿病等优点,被公认为是一种保健食品。目前全世界保存20多万份燕麦及其野生种资源,中国国家种质库保存了4800多份燕麦种质资源。为促进燕麦在种质创新和育种中的利用,有必要加强燕麦遗传资源评价研究,挖掘有用的种质材料。本研究以来自五个国家即巴西、加拿大、中国、蒙古和美国的286份燕麦种质为试验材料,应用表型和基因型分析手段,研究了燕麦种质性状的形态变异和分子遗传多样性,探讨了不同国家燕麦种质的遗传结构和亲缘关系,发现了一批重要农艺性状的分子标记和QTLs。主要研究结果如下:1、表型鉴定:燕麦种质的八个形态特征差异显著。分蘖数最多的品种(6.77)来自巴西;株高最高的品种(98.19cm)来自中国,最矮的(72.13cm)来自巴西;穗长最长(16.57cm)、小穗数最多(49.75)和旗叶长最长(26.10cm)的品种均来自蒙古。加拿大的材料具有较高的单株粒重(8.14...
【文章页数】：87 页
【学位级别】：博士
【文章目录】：
博士学位论文评阅人、答辩委员会签名表
摘要
Abstract
Abbreviations英文缩略表
Chapter Ⅰ Introduction
1.1 Importance of oat
1.2 Distribution and production of oat
1.2.1 Distribution and production in the world
1.2.2 Distribution and production in China
1.3 Origin and classification of oat
1.3.1 Taxonomy
1.3.2 Origin and evolution
1.3.3 Primary and secondary centers of diversity
1.4. Germplasm resources of oat
1.4.1 Germplasm collections
1.4.2 Phenotypic variation of oat germplasm resources
1.5 Molecular markers
1.5.1 RFLPs
1.5.2 RAPDs
1.5.3 AFLPs
1.5.4 SSRs
1.5.5 DAr Ts
1.5.6 SNPs
1.6 Genotypic diversity of oat assessed by molecular markers
1.7 Construction of genetic linkage maps
1.8 Association analysis of morphological traits and molecular markers
1.9 The importance and objectives of the research
1.9.1 Importance of the research
1.9.2 Objectives of the research
1.9.3 Technical road map
Chapter Ⅱ Characterization of Morphological Traits of Oat Accessions
2.1 Materials and methods
2.1.1 plant materials
2.1.2 Field experiments
2.1.3 Data Analysis
2.2 Results
2.2.1 Basic statistics of morphological traits
2.2.2 Principle Component Analysis
2.2.3 Correlations between morphological traits of oat accessions
2.2.4 Classification and characteristics of oat accessions
2.3 Discussions
2.3.1 Morphological variation
2.3.2 Correlations and Principle Component Analysis of morphological traits
2.3.3 Classification and characteristics of oat accessions
Chapter Ⅲ Assessment of Genetic Diversity in Oat Accessions by SSR Markers
3.1 Materials and methods
3.1.1 Plant materials
3.1.2 DNA Isolation and PCR amplification
3.1.3 Allele scoring
3.1.4 Data analysis
3.2 Results
3.2.1 SSR marker polymorphism
3.2.2 Genetic variation among oat accessions
3.2.3 Genetic relationships among oat accessions
3.2.4 Genetic structure of oat accessions
3.3 Discussions
3.3.1 SSR Polymorphism and allelic frequencies
3.3.2 Genetic variation and relationships of oat accessions from different countries
Chapter Ⅳ Phenotypic-Genotypic Association Analysis on Oat Germplasm
4.1 Materials and methods
4.1.1 Plant materials
4.1.2 Data records
4.1.3 Data analysis
4.2 Results
4.3 Discussions
4.3.1 Reliable relations between morphological traits and molecular markers
4.3.2 Traits with multiple markers
4.3.3 Co-located markers for traits
Chapter Ⅴ Construction of Genetic Linkage Map and Analysis of QTLs forNaked Grain Type in Oat
5.1 Materials and Methods
5.1.1 Construction of F2 population
5.1.2 Phenotypic identification of F2 population
5.1.3 DNA extraction and analyses of SSR genotype
5.1.4 The Data Analysis
5.2 Results
5.2.1 Trait performance of parents and F2 population
5.2.2 Construction of genetic linkage map
5.2.3 QTL analysis
5.3 Discussions
5.3.1 Genetic linkage map with SSR markers
5.3.2 QTLs on naked grain type and associated markers
5.3.3 Oat genetic linkage groups and QTLs
Chapter Ⅵ Overall Conclusions
6.1 Phenotypic variation of oat accessions
6.2 Genetic diversity of oat accessions revealed by SSR markers
6.3 Phenotypic-genotypic association of oat germplasm
6.4 Genetic linkage map and QTL analysis on naked grain type
References
Annexes
Acknowledgements
Author Resume
本文编号：3973885
【文章页数】：87 页
【学位级别】：博士
【文章目录】：
博士学位论文评阅人、答辩委员会签名表
摘要
Abstract
Abbreviations英文缩略表
Chapter Ⅰ Introduction
1.1 Importance of oat
1.2 Distribution and production of oat
1.2.1 Distribution and production in the world
1.2.2 Distribution and production in China
1.3 Origin and classification of oat
1.3.1 Taxonomy
1.3.2 Origin and evolution
1.3.3 Primary and secondary centers of diversity
1.4. Germplasm resources of oat
1.4.1 Germplasm collections
1.4.2 Phenotypic variation of oat germplasm resources
1.5 Molecular markers
1.5.1 RFLPs
1.5.2 RAPDs
1.5.3 AFLPs
1.5.4 SSRs
1.5.5 DAr Ts
1.5.6 SNPs
1.6 Genotypic diversity of oat assessed by molecular markers
1.7 Construction of genetic linkage maps
1.8 Association analysis of morphological traits and molecular markers
1.9 The importance and objectives of the research
1.9.1 Importance of the research
1.9.2 Objectives of the research
1.9.3 Technical road map
Chapter Ⅱ Characterization of Morphological Traits of Oat Accessions
2.1 Materials and methods
2.1.1 plant materials
2.1.2 Field experiments
2.1.3 Data Analysis
2.2 Results
2.2.1 Basic statistics of morphological traits
2.2.2 Principle Component Analysis
2.2.3 Correlations between morphological traits of oat accessions
2.2.4 Classification and characteristics of oat accessions
2.3 Discussions
2.3.1 Morphological variation
2.3.2 Correlations and Principle Component Analysis of morphological traits
2.3.3 Classification and characteristics of oat accessions
Chapter Ⅲ Assessment of Genetic Diversity in Oat Accessions by SSR Markers
3.1 Materials and methods
3.1.1 Plant materials
3.1.2 DNA Isolation and PCR amplification
3.1.3 Allele scoring
3.1.4 Data analysis
3.2 Results
3.2.1 SSR marker polymorphism
3.2.2 Genetic variation among oat accessions
3.2.3 Genetic relationships among oat accessions
3.2.4 Genetic structure of oat accessions
3.3 Discussions
3.3.1 SSR Polymorphism and allelic frequencies
3.3.2 Genetic variation and relationships of oat accessions from different countries
Chapter Ⅳ Phenotypic-Genotypic Association Analysis on Oat Germplasm
4.1 Materials and methods
4.1.1 Plant materials
4.1.2 Data records
4.1.3 Data analysis
4.2 Results
4.3 Discussions
4.3.1 Reliable relations between morphological traits and molecular markers
4.3.2 Traits with multiple markers
4.3.3 Co-located markers for traits
Chapter Ⅴ Construction of Genetic Linkage Map and Analysis of QTLs forNaked Grain Type in Oat
5.1 Materials and Methods
5.1.1 Construction of F2 population
5.1.2 Phenotypic identification of F2 population
5.1.3 DNA extraction and analyses of SSR genotype
5.1.4 The Data Analysis
5.2 Results
5.2.1 Trait performance of parents and F2 population
5.2.2 Construction of genetic linkage map
5.2.3 QTL analysis
5.3 Discussions
5.3.1 Genetic linkage map with SSR markers
5.3.2 QTLs on naked grain type and associated markers
5.3.3 Oat genetic linkage groups and QTLs
Chapter Ⅵ Overall Conclusions
6.1 Phenotypic variation of oat accessions
6.2 Genetic diversity of oat accessions revealed by SSR markers
6.3 Phenotypic-genotypic association of oat germplasm
6.4 Genetic linkage map and QTL analysis on naked grain type
References
Annexes
Acknowledgements
Author Resume
本文编号：3973885
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