干露和产卵过程中皱纹盘鲍的耐受性和免疫应答研究
发布时间：2021-01-12 01:28
皱纹盘鲍在中国是一种重要的海水养殖经济物种,国内市场需求量大。但是,活体鲍鱼的运输仍有诸多瓶颈,通常是采用密闭的保温箱,但因运输时间较长,会对鲍鱼产生一定的胁迫应激,导致运输过程中肉质的改变甚至死亡。因此,本研究通过评估鲍在不同条件下的存活率和体重损失率（WLR）,从而确定鲍活体运输的最佳条件。模拟鲍活体运输的参数有温度、湿度和氧气等。研究结果表明,在经过冷水刺激后,一定湿度环境下鲍的存活率显著升高,而氧气供给对鲍鱼存活率无显著影响。另外,随着干露时间的增加WLR显著升高。通过检测不同干露时间（2,6,12,24和30 h）和不同干露温度（5,10,20和28℃）下的总血细胞数（THC）、血细胞死亡率和活性氧自由基（ROS）,可用于评估干露对鲍胁迫响应的影响。研究结果表明,在5℃和10℃下干露30 h,THC显著降低;在20 ℃下干露24 h,THC显著降低。在5℃和10℃下,血细胞死亡率和ROS逐渐升高,干露30 h后均达到最高值;经过24 h恢复实验后,血细胞死亡率和ROS有所降低,但仍然显著高于对照组。因此,这些参数可以作为鲍活体运输对功能性免疫相应和生理胁迫影响的指标。另外,对...
【文章来源】：厦门大学福建省 211工程院校 985工程院校 教育部直属院校
【文章页数】：120 页
【学位级别】：硕士
【文章目录】：
Abstract
摘要
CHAPTER 1. GENERAL INTRODUCTION
1.1. Biological overview of Pacific abalone
1.1.1. General characteristics, habitat and distribution
1.1.2. The circulation system and immunology system
1.1.2.1. Blood circulation
1.1.2.2. Blood chemistry baseline values
1.1.2.3. Immunology system
1.1.3. Respiration system
1.1.3.1. Aerobic respiration and anaerobic respiration
1.1.4. Reproductive system
1.2. Status abalone in China
1.2.1. China's abalone market and transportation
1.2.2. The effects of transport stress
Defining stress
The research purpose
CHAPTER 2. PHYSIOLOGICAL ASSESSMENT OF LIVE PACIFICABALONEIN DIFFERENT TRANSPORT PACKAGING
2.1. Introduction
2.2. Methodologies
2.2.1. Animals sample
2.2.2. Equipment
2.2.3. Experimental design
2.2.4. Statistical analysis
2.3. Results
2.3.1. Survival rate
2.3.2. Weight loss rate
2.4. Discussion
2.4.1. Survival rate
2.4.2. Weight loss rate
CHAPTER 3. HPLC FOR DETERMINATION ORGANIC ACIDS INANAEROBIC RESPIRATION IN PACIFIC ABALONE HEMOLYMPHDURING AIR EXPOSURE TRANSPORT
3.1. Introduction
3.2. Methodologies
3.2.1. Animals sample
3.2.2. Chemical reagents
3.2.3. Equipment
3.2.4. Experimental design
3.2.5. Methods
3.2.6. Data analysis
3.3. Results
3.3.1. Blood pyruvate and blood lactate in H. discus hannai hemolymph
3.4. Discussion
CHAPTER 4. IMPACTS OF AIR EXPOSURE ON THE IMMUNERESPONSE IN HALIOTIS DISCUS HANNAI DURING SIMULATEDLIVE TRANSPORT
4.1. Introduction
4.2. Methodologies
4.2.1. Animals sample
4.2.2. Chemical reagents
4.2.3. Equipment
4.2.4. Methods
4.2.4.1. Light microscope
4.2.4.2. Total hemocyte count （THC）
4.2.4.3. Haemocyte Mortality
4.2.4.4. Reactive oxygen species （ROS）
4.3. Results
4.3.1. Light microscopy
4.3.2. Total hemocytes count （THC）
4.3.3. Haemocyte mortality
4.3.4. Reactive oxygen species （ROS）
4.4. Discussion
4.4.1. Light microscope
4.4.2. Immune response
CHAPTER 5. MORPHOLOGICAL CHANGES IN GONADS ANDTHE IMMUNE REPONSE OF HALIOTIS DISCUS HANNAI DURINGSPAWNING PERIOD
Part 5.1. Gonad morphological
5.1.1. Introduction
5.1.2. Methodologies
5.1.2.1 Animals sample
5.1.2.2. Chemical reagents
5.1.2.3. Equipment
5.1.3. Results
Part 5.2. Effects of immune response during spawning period
5.2.1. Methodologies
5.2.1.1. Animals sample
5.2.1.2. Chemical reagents
5.2.1.3. Equipment
5.2.1.4. Methods
5.2.1.4.1. Total hemocyte count（THC）
5.2.1.4.2. Hemocyte Mortality
5.2.1.4.3. Reactive oxygen species
5.2.1.5. Statistical analyses
5.2.2. Results
5.2.2.1. Total hemocytes count
5.2.2.2. Hemocyte Mortality
5.2.2.3. Reactive oxygen species
5.2.3. Discussion
5.2.3.1. Morphological changes in gonads
5.2.3.2. Immune response
CHAPTER 6. SUMMARY AND FUTURE RESEARCH
6.1. Summary
6.2. Future research
References
Appendix 1. Changes in muscle of H. discus hannai after exposure to airimmediately from 20 to 5 ℃ for live transport simulation
Appendix 2. Changes in muscle of H. discus hannai after exposure to airimmediately from 20 to 10 ℃ for live transport simulation
Appendix 3. Changes in muscle of H. discus hannai after exposure to airimmediately from 20 to 20 ℃ for live transport simulation
Appendix 4. Changes in muscle of H. discus hannai after exposure to airimmediately from 20 to 28 ℃ for live transport simulation
Appendix 5. Representative flow cytometry analysis scatter plot of fixedhemocytes from H. discus hannai. Size against internal complexity densityplot of hemocyte positive for SYBR Green I staining. Two populations ofhemocytes were distinguishable: hyalinoc
Appendix 6. Determined hemocyte mortality by flow cytometry analysisof H. discus hannai hemocytes positive for propidium iodide （PI）
Appendix 7. Determination of oxidative activity with DCEH-DA in eachhemocyte population by flow cytometry analysis
Achievements
Acknowledgement
Biography
本文编号：2971861
【文章来源】：厦门大学福建省 211工程院校 985工程院校 教育部直属院校
【文章页数】：120 页
【学位级别】：硕士
【文章目录】：
Abstract
摘要
CHAPTER 1. GENERAL INTRODUCTION
1.1. Biological overview of Pacific abalone
1.1.1. General characteristics, habitat and distribution
1.1.2. The circulation system and immunology system
1.1.2.1. Blood circulation
1.1.2.2. Blood chemistry baseline values
1.1.2.3. Immunology system
1.1.3. Respiration system
1.1.3.1. Aerobic respiration and anaerobic respiration
1.1.4. Reproductive system
1.2. Status abalone in China
1.2.1. China's abalone market and transportation
1.2.2. The effects of transport stress
Defining stress
The research purpose
CHAPTER 2. PHYSIOLOGICAL ASSESSMENT OF LIVE PACIFICABALONEIN DIFFERENT TRANSPORT PACKAGING
2.1. Introduction
2.2. Methodologies
2.2.1. Animals sample
2.2.2. Equipment
2.2.3. Experimental design
2.2.4. Statistical analysis
2.3. Results
2.3.1. Survival rate
2.3.2. Weight loss rate
2.4. Discussion
2.4.1. Survival rate
2.4.2. Weight loss rate
CHAPTER 3. HPLC FOR DETERMINATION ORGANIC ACIDS INANAEROBIC RESPIRATION IN PACIFIC ABALONE HEMOLYMPHDURING AIR EXPOSURE TRANSPORT
3.1. Introduction
3.2. Methodologies
3.2.1. Animals sample
3.2.2. Chemical reagents
3.2.3. Equipment
3.2.4. Experimental design
3.2.5. Methods
3.2.6. Data analysis
3.3. Results
3.3.1. Blood pyruvate and blood lactate in H. discus hannai hemolymph
3.4. Discussion
CHAPTER 4. IMPACTS OF AIR EXPOSURE ON THE IMMUNERESPONSE IN HALIOTIS DISCUS HANNAI DURING SIMULATEDLIVE TRANSPORT
4.1. Introduction
4.2. Methodologies
4.2.1. Animals sample
4.2.2. Chemical reagents
4.2.3. Equipment
4.2.4. Methods
4.2.4.1. Light microscope
4.2.4.2. Total hemocyte count （THC）
4.2.4.3. Haemocyte Mortality
4.2.4.4. Reactive oxygen species （ROS）
4.3. Results
4.3.1. Light microscopy
4.3.2. Total hemocytes count （THC）
4.3.3. Haemocyte mortality
4.3.4. Reactive oxygen species （ROS）
4.4. Discussion
4.4.1. Light microscope
4.4.2. Immune response
CHAPTER 5. MORPHOLOGICAL CHANGES IN GONADS ANDTHE IMMUNE REPONSE OF HALIOTIS DISCUS HANNAI DURINGSPAWNING PERIOD
Part 5.1. Gonad morphological
5.1.1. Introduction
5.1.2. Methodologies
5.1.2.1 Animals sample
5.1.2.2. Chemical reagents
5.1.2.3. Equipment
5.1.3. Results
Part 5.2. Effects of immune response during spawning period
5.2.1. Methodologies
5.2.1.1. Animals sample
5.2.1.2. Chemical reagents
5.2.1.3. Equipment
5.2.1.4. Methods
5.2.1.4.1. Total hemocyte count（THC）
5.2.1.4.2. Hemocyte Mortality
5.2.1.4.3. Reactive oxygen species
5.2.1.5. Statistical analyses
5.2.2. Results
5.2.2.1. Total hemocytes count
5.2.2.2. Hemocyte Mortality
5.2.2.3. Reactive oxygen species
5.2.3. Discussion
5.2.3.1. Morphological changes in gonads
5.2.3.2. Immune response
CHAPTER 6. SUMMARY AND FUTURE RESEARCH
6.1. Summary
6.2. Future research
References
Appendix 1. Changes in muscle of H. discus hannai after exposure to airimmediately from 20 to 5 ℃ for live transport simulation
Appendix 2. Changes in muscle of H. discus hannai after exposure to airimmediately from 20 to 10 ℃ for live transport simulation
Appendix 3. Changes in muscle of H. discus hannai after exposure to airimmediately from 20 to 20 ℃ for live transport simulation
Appendix 4. Changes in muscle of H. discus hannai after exposure to airimmediately from 20 to 28 ℃ for live transport simulation
Appendix 5. Representative flow cytometry analysis scatter plot of fixedhemocytes from H. discus hannai. Size against internal complexity densityplot of hemocyte positive for SYBR Green I staining. Two populations ofhemocytes were distinguishable: hyalinoc
Appendix 6. Determined hemocyte mortality by flow cytometry analysisof H. discus hannai hemocytes positive for propidium iodide （PI）
Appendix 7. Determination of oxidative activity with DCEH-DA in eachhemocyte population by flow cytometry analysis
Achievements
Acknowledgement
Biography
本文编号：2971861
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