1 H NMR-Based Metabolomics Approach Reveals Metabolic Alter

发布时间：2021-08-23 20:10

　　团头鲂（Megalobrama amblycephala）是中国重要的草食性鱼类之一,年总产量位居中国养殖鱼类种类的第七位,因而其饲料的消耗量同样位居单一养殖种类的前列。目前鱼类的能量饲料来源逐渐由蛋白质饲料向非蛋白能量饲料转变。在水产动物饲料生产中,糖类和脂肪可以部分的替代蛋白质。鱼饲料中高水平糖类和脂肪的添加能减少蛋白质的浪费,但过多也会造成代谢疾病以及代谢紊乱的产生。日粮中高脂和高糖是许多疾病的影响因素,两者均能对多种代谢通路、酶的调节以及器官功能有反作用,但由不平衡日粮引起的鱼类代谢性改变的研究鲜有报道。本研究以团头鲂为研究对象,经过8周的高糖高脂饲料饲养,利用1H NMR代谢组学方法,评估高脂日粮（HFD）、高糖日粮（HCD）和高脂高糖日粮（HFHCD）对团头鲂血清和肝脏中代谢产物的影响,同时对代谢产物进行了比较分析,揭示它们对应的代谢路径。最终通过对血清指标的生化分析来进一步佐证高糖高脂日粮对团头鲂能量代谢的影响,其主要结果如下:1.在高脂日粮组（HFD）,与对照组相比,除高密度脂蛋白（HDL）外,血清中甘油三酯（TG）、胆固醇（TCHOL）、低密度脂蛋白（LDL）、谷丙转... 

【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校

【文章页数】：162 页

【学位级别】：博士

【文章目录】：
摘要
ABSTRACT
ABBREVIATIONS
CHAPTER 1 REVIEW OF LITERATURE
    1.1 Introduction of blunt snout bream
        1.1.1 Biological characteristics
        1.1.2 Situation of high fat and high carbohydrate used in feed industry
        1.1.3 Important to research
    1.2 Metabolomics study
        1.2.1 What is metabolomics?
        1.2.2 Advantage of metabolomics compared with other technology
        1.2.3 Applications of metabolomics
    1.3 Fat diet and its metabolism in fish
        1.3.1 Fat characteristics
        1.3.2 Function in animals
        1.3.3 Pathway and impact factors of lipid metabolism
    1.4 Carbohydrate diet and its metabolism in fish
        1.4.1 Carbohydrate characteristics
        1.4.2 Function in animals
        1.4.3 Pathway and impact factors of carbohydrate metabolism
CHAPTER 2 METABOLIC ALTERATIONS AFFECTED BY HIGH FAT DIET
    2.1 Introduction
    2.2 Materials and methods
        2.2.1 Fish and samples for experiment
        2.2.2 Serum biochemical analysis
        2.2.3 Metabolomics study by NMR
    2.3 Results
        2.3.1 Serum biochemical alterations affected by high fat diet
        2.3.2 Metabolic alterations affected by high fat diet
    2.4 Discussions
        2.4.1 Serum biochemical alterations affected by high fat diet
        2.4.2 Metabolic alterations affected by high fat diet
CHAPTER 3 METABOLIC ALTERATIONS AFFECTED BY HIGH CARBOHYDRATE DIET
    3.1 Introduction
    3.2 Materials and methods
        3.2.1 Fish and samples for experiment
        3.2.2 Serum biochemical analysis
        3.2.3 Metabolomics study by NMR
    3.3 Results
        3.3.1 Serum biochemical alterations affected by high carbohydrate diet
        3.3.2 Metabolic alterations affected by high carbohydrate diet
    3.4 Discussion
        3.4.1 Serum biochemical alterations affected by high carbohydrate diet
        3.4.2 Metabolic alterations affected by high carbohydrate diet
CHAPTER 4 METABOLIC ALTERATIONS AFFECTED BY HIGH-FAT HIGH- CARBOHYDRATE DIET
    4.1 Introduction
    4.2 Materials and methods
        4.2.1 Fish and samples for experiment
        4.2.2 Serum biochemical analysis
        4.2.3 Metabolomics study by NMR
    4.3 Results
        4.3.1 Serum biochemical alterations affected by high-fat high- carbohydrate diet
        4.3.2 Metabolic alterations affected by high-fat high- carbohydrate diet
    4.4 Discussions
        4.4.1 Serum biochemical alterations affected by high-fat high- carbohydrate diet
        4.4.2 Metabolic alterations affected by high-fat high- carbohydrate diet
CHAPTER 5 PAIRWISE GROUP COMPARISON OF METABOLOMICS STUDY
    5.1 Introduction
    5.2 Materials and methods
        5.2.1 Metabolic alterations affected by high carbohydrate compared with high fatdiet
        5.2.2 Metabolic alterations affected by high-fat high- carbohydrate diet comparedwith high fat diet
        5.2.3 Metabolic alterations affected by high-fat high- carbohydrate diet comparedwith high carbohydrate diet
    5.3 Results
        5.3.1 Metabolic alterations affected by high carbohydrate compared with high fatdiet
        5.3.2 Metabolic alterations affected by high-fat high- carbohydrate diet comparedwith high fat diet
        5.3.3 Metabolic alterations affected by high-fat high- carbohydrate diet comparedwith high carbohydrate diet
    5.4 Discussion
        5.4.1 Metabolic alterations affected by high carbohydrate compared with high fatdiet
        5.4.2 Metabolic alterations affected by high-fat high- carbohydrate diet comparedwith high fat diet
        5.4.3 Metabolic alterations affected by high-fat high- carbohydrate diet comparedwith high carbohydrate diet
CHAPTER 6 CONCLUSIONS
REFERENCES
APPENDIX 1 METABOLOMICS PROFILE OF MEGALOBRAMA AMBLYCEPHALA BY USINGMETABOLOMICS
APPENDIX 2 PUBLICATIONS
APPENDIX 3 AWARDS AND FUNDING
ACKNOWLEDGEMENTS


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