植核后马氏珠母贝黑壳色选育群体与对照群体免疫基因表达量与育珠性能比较
发布时间：2021-09-07 09:02
马氏珠母贝（Pinctada fucata martensii）是生产海水珍珠主要物种。通过植核手术生产珍珠在世界范围内备受关注,马氏珠母贝生产的珍珠占珍珠产量的90%以上。我们通过群体选育培育了红、黄、黑与白壳色群体。前期研究表明,红色、黄色、黑色、白色和棕色（最常见）壳色群体表现出不同的生长和繁殖能力,例如植入核后黑色壳色群体具有较高的成活率、留核率等。本研究中,以对照养殖群体为参考,比较了植核后黑壳色群体免疫相关基因的表达量、免疫酶活力与产珠性能差异。1.黑壳色群体和对照群体植核后的存活率、留核率和免疫酶活性比较本研究比较了BG和CG的总类胡萝卜素含量（TCC）。对BG进行了植核实验。比较移植后不同时间点BG与CG的存活率、留珠率、免疫力和抗氧化能力。结果表明,BG组的TCC显著大于对照组（P<0.05）。植核后第7、30、360天,BG组存活率和留珠率均显著高于CG组（P<0.05）。360天时,BS组珍珠厚度显著大于CG组（P<0.05）。BG在植核后0h、12h、1d、3d、5d、7d和30d的ACP、AKP、SOD、CAT、TAOC和LZ活性均高于CG组...
【文章来源】：广东海洋大学广东省
【文章页数】：97 页
【学位级别】：硕士
【文章目录】：
Abbreviations
Abstract
摘要
1 General Introduction
1.1 Background of the Study
1.2 Literature Review
1.2.1 Pearl Oyster Immunity
1.2.2 Nucleus Insertion Operation
1.2.3 Pigments and Immunity
1.2.4 Development of Selected Line(Colored Stock)
1.3 Problem Statement and Scientific Significance of the Study
1.4 Research Content and Goals
2 Survival,retention rate and immunity of the black shell colored and control stocks of pearl oyster Pinctada fucata martensii after grafting operation
2.1 Introduction
2.2 Materials and Methods
2.2.1 Grafting Experiments
2.2.2 Sample Collection
2.2.3 Total Carotenoid Content(TCC)
2.2.4 Survival and Retention Rates
2.2.5 Activities of Immune-and Antioxidant-related Enzymes
2.2.6 Immune and Antioxidant-related Gene Expression
2.2.7 Statistical Analysis
2.3 Results
2.3.1 Total Carotenoid Content
2.3.2 Survival and Retention Rate after Grafting
2.3.3 Activities of Immune-and Antioxidant-related Enzymes
2.3.4 Immune and Antioxidant-related Gene Expression
2.4 Discussion
2.4.1 Total Carotenoid Content(TCC)
2.4.2 Survival and Retention Rate
2.4.3 Immune and Antioxidant-related Enzyme Activity
2.4.4 Gene Expression
3 A comparison of pearl productions performance,enzymatic activity and immune related gene expression of different aged pearl oyster Pinctada fucata martensii
3.1 Introduction
3.2 Material and Methods
3.2.1 Experimental Animals
3.2.2 Experimental Design
3.2.3 Sample Collection
3.2.4 Immune and Antioxidant-related Enzyme Activity
3.2.5 Gene Expression Variation
3.2.6 Statistical Analysis
3.3 Results
3.3.1 Comparison of Pearl Production Traits
3.3.2 Immune and Antioxidant-related Enzyme Activity
3.3.3 Expression of Immune and Antioxidant Enzyme Genes
3.3.4 Expression of Biomineralization Genes
3.4 Discussion
3.4.1 Immunity and Antioxidant Capacity
3.4.2 Biomineralization Capacity
4 Characterization of thioredoxin-like protein-5 and its differential expression in the black colored selected line and control stocks of Pinctada fucata martensii to grafting operation
4.1 Introduction
4.2 Materials and Methods
4.2.1 Grafting Operation and Sample Collection
4.2.2 RNA Extraction
4.2.3 Gene Cloning and Sequencing
4.2.4 Bioinformatics Analysis
4.2.5 Gene Expression in Various Tissues
4.2.6 Gene Expression in Response to the Grafting Operation
4.2.7 Gene Function Identification
4.3 Results
4.3.1 Cloning and Sequence Analysis of Pm Trxlp-5
4.3.2 Structural and Homologous Analysis
4.3.3 Protein Structure
4.3.4 mRNA Expression of Pm Trxlp in Various Tissues
4.3.5 mRNA Expression of Pm Trxlp-5 after Immune Stimulation
4.3.6 mRNA Expression of Pm Trxlp-5 after Grafting Operation
4.4 Discussion
5 Characterization of cyclin dependent kinase-7 and its differential expression in the black colored selected line and control stock of Pinctada fucata martensii to grafting operation
5.1 Introduction
5.2 Materials and Methods
5.2.1 Grafting Operation and Sample Collection
5.2.2 RNA Extraction
5.2.3 Gene Cloning and Sequencing
5.2.4 Bioinformatics Analysis
5.2.5 Gene Expression in Various Tissues
5.2.6 Gene Expression in Response to the Grafting Operation
5.2.7 Gene Function Identification
5.3 Results and Discussion
5.3.1 Cloning and Sequence Analysis of PmCdk-7
5.3.2 Structural and Homologous Analysis
5.3.3 Protein Structure
5.3.4 mRNA Expression of PmCdk-7 in Various Tissues
5.3.5 mRNA Expression of PmCdk-7 after Grafting Operation
5.3.6 mRNA Expression of PmCdk-7 after Immune Stimulation
6 General Conclusion
References
Acknowledgement
Author's Profile
SUPERVISORS PROFILE导师简介
【参考文献】：
期刊论文
[1]马氏珠母贝微胶囊饲料的适用性研究[J]. 杨创业,罗少杰,王庆恒,邓岳文,杜晓东. 中国水产科学. 2015(03)
[2]高温对虾夷扇贝体腔液免疫酶活力的影响[J]. 贲月,郝振林,丁君,常亚青. 水产学报. 2013(06)
[3]利用马氏珠母贝4个壳色系F3培育厚层优质珍珠[J]. 符韶,谢绍河,邓岳文,梁飞龙. 水产学报. 2012(09)
[4]贝龄对马氏珠母贝植核贝生长、成活率和育珠性状的影响[J]. 尹立鹏,邓岳文,杜晓东,王庆恒. 中国水产科学. 2012(04)
[5]Growth performance and physiological parameters of the second generation selected and control groups of Pinctada martensii[J]. DENG Yuewen 1,YU Ziniu 2,DU Xiaodong 1 WANG Qingheng 1,FU Shao 1 1 Fisheries College,Guangdong Ocean University,Zhanjiang 524025,China 2 South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China. Acta Oceanologica Sinica. 2011(02)
[6]马氏珠母贝饥饿补偿生长的初步研究[J]. 何毛贤,袁涛,黄良民. 热带海洋学报. 2010(06)
[7]马氏珠母贝珍珠囊发育的组织和组织化学研究[J]. 焦钰,师尚丽,杜晓东,逯云召,王庆恒. 广东海洋大学学报. 2010(04)
[8]插核手术对三角帆蚌血淋巴中3种免疫防御因子的影响[J]. 何秀娟,施志仪,李文娟. 中国水产科学. 2009(06)
[9]三角帆蚌alpha-2巨球蛋白cDNA全长的克隆及表达特征[J]. 施志仪,杨显祥,陈晓武,李勇. 水产学报. 2008(04)
[10]珍珠囊发育中马氏珠母贝血清免疫因子的变化规律[J]. 焦钰,杜晓东,师尚丽,逯云召,朱文皓. 广东海洋大学学报. 2007(04)
本文编号：3389265
【文章来源】：广东海洋大学广东省
【文章页数】：97 页
【学位级别】：硕士
【文章目录】：
Abbreviations
Abstract
摘要
1 General Introduction
1.1 Background of the Study
1.2 Literature Review
1.2.1 Pearl Oyster Immunity
1.2.2 Nucleus Insertion Operation
1.2.3 Pigments and Immunity
1.2.4 Development of Selected Line(Colored Stock)
1.3 Problem Statement and Scientific Significance of the Study
1.4 Research Content and Goals
2 Survival,retention rate and immunity of the black shell colored and control stocks of pearl oyster Pinctada fucata martensii after grafting operation
2.1 Introduction
2.2 Materials and Methods
2.2.1 Grafting Experiments
2.2.2 Sample Collection
2.2.3 Total Carotenoid Content(TCC)
2.2.4 Survival and Retention Rates
2.2.5 Activities of Immune-and Antioxidant-related Enzymes
2.2.6 Immune and Antioxidant-related Gene Expression
2.2.7 Statistical Analysis
2.3 Results
2.3.1 Total Carotenoid Content
2.3.2 Survival and Retention Rate after Grafting
2.3.3 Activities of Immune-and Antioxidant-related Enzymes
2.3.4 Immune and Antioxidant-related Gene Expression
2.4 Discussion
2.4.1 Total Carotenoid Content(TCC)
2.4.2 Survival and Retention Rate
2.4.3 Immune and Antioxidant-related Enzyme Activity
2.4.4 Gene Expression
3 A comparison of pearl productions performance,enzymatic activity and immune related gene expression of different aged pearl oyster Pinctada fucata martensii
3.1 Introduction
3.2 Material and Methods
3.2.1 Experimental Animals
3.2.2 Experimental Design
3.2.3 Sample Collection
3.2.4 Immune and Antioxidant-related Enzyme Activity
3.2.5 Gene Expression Variation
3.2.6 Statistical Analysis
3.3 Results
3.3.1 Comparison of Pearl Production Traits
3.3.2 Immune and Antioxidant-related Enzyme Activity
3.3.3 Expression of Immune and Antioxidant Enzyme Genes
3.3.4 Expression of Biomineralization Genes
3.4 Discussion
3.4.1 Immunity and Antioxidant Capacity
3.4.2 Biomineralization Capacity
4 Characterization of thioredoxin-like protein-5 and its differential expression in the black colored selected line and control stocks of Pinctada fucata martensii to grafting operation
4.1 Introduction
4.2 Materials and Methods
4.2.1 Grafting Operation and Sample Collection
4.2.2 RNA Extraction
4.2.3 Gene Cloning and Sequencing
4.2.4 Bioinformatics Analysis
4.2.5 Gene Expression in Various Tissues
4.2.6 Gene Expression in Response to the Grafting Operation
4.2.7 Gene Function Identification
4.3 Results
4.3.1 Cloning and Sequence Analysis of Pm Trxlp-5
4.3.2 Structural and Homologous Analysis
4.3.3 Protein Structure
4.3.4 mRNA Expression of Pm Trxlp in Various Tissues
4.3.5 mRNA Expression of Pm Trxlp-5 after Immune Stimulation
4.3.6 mRNA Expression of Pm Trxlp-5 after Grafting Operation
4.4 Discussion
5 Characterization of cyclin dependent kinase-7 and its differential expression in the black colored selected line and control stock of Pinctada fucata martensii to grafting operation
5.1 Introduction
5.2 Materials and Methods
5.2.1 Grafting Operation and Sample Collection
5.2.2 RNA Extraction
5.2.3 Gene Cloning and Sequencing
5.2.4 Bioinformatics Analysis
5.2.5 Gene Expression in Various Tissues
5.2.6 Gene Expression in Response to the Grafting Operation
5.2.7 Gene Function Identification
5.3 Results and Discussion
5.3.1 Cloning and Sequence Analysis of PmCdk-7
5.3.2 Structural and Homologous Analysis
5.3.3 Protein Structure
5.3.4 mRNA Expression of PmCdk-7 in Various Tissues
5.3.5 mRNA Expression of PmCdk-7 after Grafting Operation
5.3.6 mRNA Expression of PmCdk-7 after Immune Stimulation
6 General Conclusion
References
Acknowledgement
Author's Profile
SUPERVISORS PROFILE导师简介
【参考文献】：
期刊论文
[1]马氏珠母贝微胶囊饲料的适用性研究[J]. 杨创业,罗少杰,王庆恒,邓岳文,杜晓东. 中国水产科学. 2015(03)
[2]高温对虾夷扇贝体腔液免疫酶活力的影响[J]. 贲月,郝振林,丁君,常亚青. 水产学报. 2013(06)
[3]利用马氏珠母贝4个壳色系F3培育厚层优质珍珠[J]. 符韶,谢绍河,邓岳文,梁飞龙. 水产学报. 2012(09)
[4]贝龄对马氏珠母贝植核贝生长、成活率和育珠性状的影响[J]. 尹立鹏,邓岳文,杜晓东,王庆恒. 中国水产科学. 2012(04)
[5]Growth performance and physiological parameters of the second generation selected and control groups of Pinctada martensii[J]. DENG Yuewen 1,YU Ziniu 2,DU Xiaodong 1 WANG Qingheng 1,FU Shao 1 1 Fisheries College,Guangdong Ocean University,Zhanjiang 524025,China 2 South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China. Acta Oceanologica Sinica. 2011(02)
[6]马氏珠母贝饥饿补偿生长的初步研究[J]. 何毛贤,袁涛,黄良民. 热带海洋学报. 2010(06)
[7]马氏珠母贝珍珠囊发育的组织和组织化学研究[J]. 焦钰,师尚丽,杜晓东,逯云召,王庆恒. 广东海洋大学学报. 2010(04)
[8]插核手术对三角帆蚌血淋巴中3种免疫防御因子的影响[J]. 何秀娟,施志仪,李文娟. 中国水产科学. 2009(06)
[9]三角帆蚌alpha-2巨球蛋白cDNA全长的克隆及表达特征[J]. 施志仪,杨显祥,陈晓武,李勇. 水产学报. 2008(04)
[10]珍珠囊发育中马氏珠母贝血清免疫因子的变化规律[J]. 焦钰,杜晓东,师尚丽,逯云召,朱文皓. 广东海洋大学学报. 2007(04)
本文编号：3389265
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