经哈维氏弧菌感染后帆纳滨对虾的鳃、肝胰腺和心脏的转录组分析

发布时间：2021-11-09 13:20

　　对虾养殖业是全球重要的粮食生产产业。现时,对虾养殖正受到许多挑战,包括疾病的爆发、抗生素的过度使用和养殖环境的恶化。这些挑战,包括在分子水平上对对虾免疫系统,现时尚未完全了解。帆纳滨对虾（L.vannamei）是全球重要的养殖品种。尽管如此,哈维氏弧菌的感染给对对虾养殖业造成了巨大的经济损失。现时,我们对对虾受哈维氏弧菌感染后的免疫反应了解甚少。因此,研究帆纳滨对虾对哈维氏弧菌感染的免疫反应,将对改善对虾健康养殖至关重要。下面总结了该研究的主要结果:1哈维氏弧菌感染后帆纳滨对虾的转录学概况研究报告揭示帆纳滨对虾在注射细菌后的腮、肝胰腺及心脏的基因转录改变的数据。RNAseq数据中总共获得了33,353个高质量的单个基因。比较基因组分析显示,在哈维氏菌感染后,有9,752个差异表达的基因（DEGs）,包括5,067个向上调节的基因和4,687个向下调控基因。选取了7个可能参与免疫的DEG,通过实时定量RT-PCR进行进一步分析,结果表明其表达模式与获得的转录组数据一致。2基因表达模式和基因表达的组织分布对虾的免疫系统,主要是通过先天免疫和细胞免疫反应识别和破坏入侵病源。目前的研究重点是先... 

【文章来源】：广东海洋大学广东省

【文章页数】：118 页

【学位级别】：硕士

【文章目录】：
Abbreviations
Abstract
摘要
1 General Introduction
    1.1 Background of the Study
    1.2 Literature Review
        1.2.1 Penaeid shrimp farming
            1.2.1.1 Culture areas of important penaeidae family in Asia
            1.2.1.2 Aquaculture production of peneid shrimp in Asia
            1.2.1.3 Constraints of penaeid shrimp aquaculture in Asia
        1.2.2 Morphology of Litopenaeus vannamei
            1.2.2.1 Gills of shrimps
            1.2.2.2 Hepatopancreas of shrimps
            1.2.2.3 Heart of shrimps
        1.2.3 Shrimp aquaculture and Vibrio infections
            1.2.3.1 Vibrio harveyi
            1.2.3.2 The disease of Vibrio harveyi
            1.2.3.3 V.harveyi pathogenicity mechanism
            1.2.3.4 Clinical signs of diseases related to V.harveyi
        1.2.4 Shrimp immunity
            1.2.4.1 Shrimp immune mechanism
            1.2.4.2 Shrimp immune-related genes
            1.2.4.3 Research progress in immune mechanisms of shrimps
                1.2.4.3.1 Toll receptor pathway
                1.2.4.3.2 Immune deficiency pathway(IMD)
                1.2.4.3.3 Express Sequence Tag/Transcriptome pathway
                1.2.4.3.4 RNAi pathway
    1.3 Problem Statement
    1.4 The Aim and Scope of the Study
    1.5 The Scientific Significance of the Study
2 Transcriptomic Analysis of the Gill,Hepatopancreas,and Heart of Litopenaeus vannamei after Vibrio harveyi infection
    2.1 Introduction
    2.2 Materials and Methods
        2.2.1 Animals
        2.2.2 Bacterial Preparation and challenge test
        2.2.3 Sample collection
        2.2.4 Library preparation for Transcriptome sequencing
        2.2.5 De novo assembly and functional annotation
        2.2.6 Differentially expressed gene and enrichment analysis
        2.2.7 Experimental validation by real-time PCR(RT-PCR)
        2.2.8 Data analysis
    2.3 Results
        2.3.1 Bacterial Preparation and challenge test
        2.3.2 Sequencing and de novo assembly
        2.3.3 Function annotation of unigenes
        2.3.4 Function annotation of the Differentially Expressed Genes
        2.3.5 Verification of DEGs from transcriptomic data by RT-PCR
    2.4 Discussion
    2.5 Conclusion
3 Tissue distribution and expression pattern analysis
    3.1 Introduction
    3.2 Materials and Methods
        3.2.1 Animals and Sampling
        3.2.2 Expression pattern of selected genes
        3.2.3 Tissue distribution of selected genes
        3.2.4 Data analysis
    3.3 Results
        3.3.1 Expression pattern
        3.3.2 Tissue distribution
    3.4 Discussion
    3.5 Conclusions
4 RNA Interference and bacterial clearance assay
    4.1 Introduction
    4.2 Materials and method
        4.2.1 Animals and Sampling
        4.2.2 Double-strand RNA(ds RNA)/RNA Interference
        4.2.3 Bacterial clearance assay
    4.3 Results
        4.3.1 Double-strand RNA(ds RNA)/RNAi
        4.3.2 Bacterial clearance assay
    4.4 Discussion
    4.5 Conclusion
5 General Conclusion and Recommendations
    5.1 Conclusion
    5.2 Recommendations
References
APPENDIX(gene sequences of selected genes)
ACKNOWLEDGEMENT
作者简介
导师简介


【参考文献】：
期刊论文
[1]Hemolymph proteins in marine crustaceans[J]. W Sylvester Fredrick,S Ravichandran.  Asian Pacific Journal of Tropical Biomedicine. 2012(06)



本文编号：3485426