高豆粕饲料中添加丁酸甘油酯对黑鲷幼鱼的影响
发布时间：2022-12-09 00:21
豆粕替代鱼粉在水产饲料生产中已取得了多方面的进展,但由于豆粕中氨基酸不平衡和抗营养因子的存在,豆粕高水平或完全替代鱼粉的效果并不理想。为了提高豆粕替代鱼粉的效果,在饲料中添加饲料添加剂是可行的方式。丁酸甘油酯是一种丁酸酯衍生物,在维持肠胃健康和提高动物生长性能方面具有重要作用。丁酸甘油酯参与动物的肠道粘膜防御、炎症反应、抗氧化、细胞生长分化以及肠道免疫调节。因此,饲料中添加丁酸甘油酯在动物生产中具有重要意义。黑鲷是中国养殖鱼类中一种名贵商品鱼,其肉质鲜美,深受消费者欢迎。该试验以黑鲷作为研究对象,以高豆粕的饲料中添加不同种类和水平的丁酸甘油酯为研究内容,研究主要结果归纳如下:1.该实验旨在研究在高豆粕基础饲料中添加不同水平的三丁酸甘油酯（tributyrin,TB）的效果。设计了七组试验饲料,包括PC（positive control）（456 g kg-1鱼粉,120 g kg-1豆粕）,NC（negative control）（209 g kg-1鱼粉,450 g kg-1豆粕）,和分别添加0.5,1,2,4,8g TB每千克NC饲料组成的TB饲料。最终,饲喂PC饲料的鱼体增重和特...
【文章页数】：159 页
【学位级别】：博士
【文章目录】：
Funding information Acknowledgements Dedication 摘要 Abstract 1 General introduction
1.1 Research background
1.2 Research significance
1.3 General objectives 2 Literature review
2.1 Overview of aquaculture and worldwide production
2.1.1 Generality
2.1.2 Feed production and nutrition methods in aquaculture
2.2 Fish meal and fish oil in aquafeed
2.3 Problematic and alternative replacement of fish meal and fish oil
2.4 Soybean meal
2.5 Overview of short chain fatty acid-Butyrate
2.5.1 Sodium butyrate
2.5.2 Monobutyrin
2.5.3 Dibutyrin
2.5.4 Tributyrin
2.6 Mechanisms and functions
2.6.1 Improve gut morphological structure
2.6.2 Enhance antioxidant capacity
2.6.3 Enhance gut immunity by alleviating inflammatory responses
2.6.4 Promote beneficial bacteria and maintain intestinal microflora balance
2.7 Application in animal production
2.7.1 Application on swine
2.7.2 Application on broiler
2.7.3 Application on ruminant
2.7.4 Application on aquatic animals
2.8 Research progress on black sea bream Acanthopagrus schlegelii
2.8.1 Classification
2.8.2 Biological characteristic
2.8.3 Nutritional requirements of black sea bream 3 Tributyrin supplementation in high soybean meal diet
3.1 Introduction
3.2 Materials and methods
3.2.1 Experimental diets formulation and composition
3.2.2 Animal husbandry, experimental site and conditions
3.2.3 Samples collection
3.2.4 Sample analytical methods
3.2.5 Digestive enzyme activity
3.2.6 Serum parameters
3.2.7 Intestinal histology
3.2.8 Gene expression analysis
3.2.9 Calculation and statistical analysis
3.3 Results
3.3.1 Growth performance and morphometric parameters
3.3.2 Feed utilization parameters
3.3.3 Apparent digestibility coefficients
3.3.4 Phosphorus discharge
3.3.5 Whole body and dorsal muscle proximate composition
3.3.6 Digestive enzyme activities parameters
3.3.7 Serum biochemical parameters
3.3.8 Serum immune and antioxidant parameters
3.3.9 Intestinal histology structure and morphometric parameters
3.3.10 Intestinal gene expression
3.4 Discussion
3.5 Chapter summary 4 Mixture of butyrate glycerides supplementation in high soybean meal diet
4.1 Introduction
4.2 Materials and methods
4.2.1 Experimental diets formulation and composition
4.2.2 Animal husbandry,experimental site and conditions
4.2.3 Samples collection
4.2.4 Sample analytical methods
4.2.5 Digestive enzyme activity
4.2.6 Serum parameters
4.2.7 Intestinal histology
4.2.8 Calculation and statistical analysis
4.3 Results
4.3.1 Growth performance and morphometric parameters
4.3.2 Feed utilization parameters
4.3.3 Whole body and dorsal muscle proximate composition
4.3.4 Digestive enzyme parameters
4.3.5 Serum biochemical parameters
4.3.6 Serum immune and antioxidative parameters
4.3.7 Intestinal histology structure and morphometric parameters
4.4 Discussion
4.5 Chapter summary 5 Comparative study on the effectiveness of using mixture of butyrate glycerides, tributyrin andmicroencapsulated sodium butyrate
5.1 Introduction
5.2 Materials and methods
5.2.1 Experimental diets formulation and composition
5.2.2 Animal husbandry, experimental site and conditions
5.2.3 Samples collection
5.2.4 Sample analytical methods
5.2.5 Intestinal histology
5.2.6 Calculation and statistical analysis
5.3 Results
5.3.1 Growth performance and morphometric parameters
5.3.2 Feed utilization parameters
5.3.3 Whole body and dorsal muscle proximate composition
5.3.4 Intestine histological structure
5.4 Discussion
5.5 Chapter summary 6 Effects of butyrate glyceride supplementation on microbiome community
6.1 Introduction
6.2 Materials and methods
6.2.1 Experimental diets formulation and composition
6.2.2 Animal husbandry, experimental site and conditions
6.2.3 Samples collection
6.2.4 Microbiota analysis
6.3 Results
6.3.1 OTUs and alpha diversity analysis
6.3.2 Microbiota community composition and relative abundance analysis
6.4 Discussion
6.5 Chapter summary 7 General conclusions and future perspectives
7.1 Conclusions
7.2 Innovation
7.3 Future perspective References
【参考文献】：
期刊论文
[1]Potential beneficial effects of butyrate in intestinal and extraintestinal diseases[J]. Roberto Berni Canani,Margherita Di Costanzo,Ludovica Leone,Monica Pedata,Rosaria Meli,Antonio Calignano. World Journal of Gastroenterology. 2011(12)
[2]怀头鲇早期发育阶段消化酶及碱性磷酸酶活性变化(英文)[J]. 刘伟,张秀梅,王立波. 动物学研究. 2010(06)
博士论文
[1]n-3高不饱和脂肪酸对黑鲷幼鱼生长及脂肪代谢的影响[D]. 马晶晶.浙江大学 2008
硕士论文
[1]饵料维生素C对黑鲷仔鱼生长和体组织生化指标的影响[D]. 王秀英.浙江大学 2004
本文编号：3714430
【文章页数】：159 页
【学位级别】：博士
【文章目录】：
Funding information Acknowledgements Dedication 摘要 Abstract 1 General introduction
1.1 Research background
1.2 Research significance
1.3 General objectives 2 Literature review
2.1 Overview of aquaculture and worldwide production
2.1.1 Generality
2.1.2 Feed production and nutrition methods in aquaculture
2.2 Fish meal and fish oil in aquafeed
2.3 Problematic and alternative replacement of fish meal and fish oil
2.4 Soybean meal
2.5 Overview of short chain fatty acid-Butyrate
2.5.1 Sodium butyrate
2.5.2 Monobutyrin
2.5.3 Dibutyrin
2.5.4 Tributyrin
2.6 Mechanisms and functions
2.6.1 Improve gut morphological structure
2.6.2 Enhance antioxidant capacity
2.6.3 Enhance gut immunity by alleviating inflammatory responses
2.6.4 Promote beneficial bacteria and maintain intestinal microflora balance
2.7 Application in animal production
2.7.1 Application on swine
2.7.2 Application on broiler
2.7.3 Application on ruminant
2.7.4 Application on aquatic animals
2.8 Research progress on black sea bream Acanthopagrus schlegelii
2.8.1 Classification
2.8.2 Biological characteristic
2.8.3 Nutritional requirements of black sea bream 3 Tributyrin supplementation in high soybean meal diet
3.1 Introduction
3.2 Materials and methods
3.2.1 Experimental diets formulation and composition
3.2.2 Animal husbandry, experimental site and conditions
3.2.3 Samples collection
3.2.4 Sample analytical methods
3.2.5 Digestive enzyme activity
3.2.6 Serum parameters
3.2.7 Intestinal histology
3.2.8 Gene expression analysis
3.2.9 Calculation and statistical analysis
3.3 Results
3.3.1 Growth performance and morphometric parameters
3.3.2 Feed utilization parameters
3.3.3 Apparent digestibility coefficients
3.3.4 Phosphorus discharge
3.3.5 Whole body and dorsal muscle proximate composition
3.3.6 Digestive enzyme activities parameters
3.3.7 Serum biochemical parameters
3.3.8 Serum immune and antioxidant parameters
3.3.9 Intestinal histology structure and morphometric parameters
3.3.10 Intestinal gene expression
3.4 Discussion
3.5 Chapter summary 4 Mixture of butyrate glycerides supplementation in high soybean meal diet
4.1 Introduction
4.2 Materials and methods
4.2.1 Experimental diets formulation and composition
4.2.2 Animal husbandry,experimental site and conditions
4.2.3 Samples collection
4.2.4 Sample analytical methods
4.2.5 Digestive enzyme activity
4.2.6 Serum parameters
4.2.7 Intestinal histology
4.2.8 Calculation and statistical analysis
4.3 Results
4.3.1 Growth performance and morphometric parameters
4.3.2 Feed utilization parameters
4.3.3 Whole body and dorsal muscle proximate composition
4.3.4 Digestive enzyme parameters
4.3.5 Serum biochemical parameters
4.3.6 Serum immune and antioxidative parameters
4.3.7 Intestinal histology structure and morphometric parameters
4.4 Discussion
4.5 Chapter summary 5 Comparative study on the effectiveness of using mixture of butyrate glycerides, tributyrin andmicroencapsulated sodium butyrate
5.1 Introduction
5.2 Materials and methods
5.2.1 Experimental diets formulation and composition
5.2.2 Animal husbandry, experimental site and conditions
5.2.3 Samples collection
5.2.4 Sample analytical methods
5.2.5 Intestinal histology
5.2.6 Calculation and statistical analysis
5.3 Results
5.3.1 Growth performance and morphometric parameters
5.3.2 Feed utilization parameters
5.3.3 Whole body and dorsal muscle proximate composition
5.3.4 Intestine histological structure
5.4 Discussion
5.5 Chapter summary 6 Effects of butyrate glyceride supplementation on microbiome community
6.1 Introduction
6.2 Materials and methods
6.2.1 Experimental diets formulation and composition
6.2.2 Animal husbandry, experimental site and conditions
6.2.3 Samples collection
6.2.4 Microbiota analysis
6.3 Results
6.3.1 OTUs and alpha diversity analysis
6.3.2 Microbiota community composition and relative abundance analysis
6.4 Discussion
6.5 Chapter summary 7 General conclusions and future perspectives
7.1 Conclusions
7.2 Innovation
7.3 Future perspective References
【参考文献】：
期刊论文
[1]Potential beneficial effects of butyrate in intestinal and extraintestinal diseases[J]. Roberto Berni Canani,Margherita Di Costanzo,Ludovica Leone,Monica Pedata,Rosaria Meli,Antonio Calignano. World Journal of Gastroenterology. 2011(12)
[2]怀头鲇早期发育阶段消化酶及碱性磷酸酶活性变化(英文)[J]. 刘伟,张秀梅,王立波. 动物学研究. 2010(06)
博士论文
[1]n-3高不饱和脂肪酸对黑鲷幼鱼生长及脂肪代谢的影响[D]. 马晶晶.浙江大学 2008
硕士论文
[1]饵料维生素C对黑鲷仔鱼生长和体组织生化指标的影响[D]. 王秀英.浙江大学 2004
本文编号：3714430
本文链接：https://www.wllwen.com/nykjlw/scyylw/3714430.html
