Induction of Autotetraploids in Poncirus Trifoliata(L.) Raf.

发布时间：2024-07-07 08:21

　　

【文章页数】：52 页

【学位级别】：硕士

【文章目录】：
Abstract
Abbreviations
1 Introduction
2 Literature review
    2.1 Types of chromosome manipulation in citrus
    2.2 The importance of polyploidy manipulating for citrus genetic improvement
    2.3 Mitotic-chromosome doubling in plant tissues
        2.3.1 Mitosis division: as a target of antimitotic doubling agents
        2.3.2 The antimitotic agents induce the chromosome manipulation
        2.3.3 Application methods of antimitotic agents for chromosome duplication
    2.4 Identification methods of the chromosome-duplicated plants
        2.4.1 The stomatal analysis
        2.4.2 Chromosome counting
        2.4.3 Morphological and developmental characteristics
        2.4.4 Flow cytometry (FCM) analysis
3 The objective
4 Materials and methods
    4.1 Plant materials and growth conditions
    4.2 Preparation of plant materials
    4.3 Colchicine treatments
    4.4 Ploidy level assessment
    4.5 Morphological observation
    4.6 Stomatal analysis
    4.7 Anatomy analysis
    4.8 Photosynthesis measurement
    4.9 Statistical analysis
5 Results
    5.1 Effects of in vitro colchicine application on the treated tissues
    5.2 Effects of in vivo colchicine treatment on the treated tissues
    5.3 Ploidy examination of regenerated shoots
    5.4 Stomatal comparison between the regenerated shoots
    5.5 Histological comparison between leaf blades of the regenerated shoots
    5.6 Morphological comparison between 2x and 4x plants
    5.7 Gas exchange comparison between diploids and tetraploids
    5.8 Chlorophyll a fluorescence comparison between 2x and 4x
6 Discussion
    6.1 The polyploidy of P.trifoliata increases the photosynthesis efficiency
    6.2 The in vivo colchicine application in citrus efficiently produce tetraploids
7 References
ACKNOWLEDGEMENTS



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