翅果油对高脂饮食诱导的非酒精性脂肪肝小鼠的改善作用
发布时间：2020-10-22 00:14
翅果油是翅果油树种仁中提取的油脂,富含不饱和脂肪酸,含量达到90%以上,其中主要是亚油酸、油酸和亚麻酸,具有很高的营养价值。本论文以翅果油作为主要原料,以C57BL/6小鼠为研究对象,探讨翅果油对高脂饮食诱导的非酒精性脂肪肝小鼠的改善作用并对干预机制做初步研究。具体内容及结果如下:(1)4周龄C57BL/6小鼠适应性喂养1周后,将40只小鼠随机分为5组(n=8):基础饮食组、高脂饮食组、翅果油低、中、高剂量组,灌胃干预8周。实验期间,记录小鼠体重和摄食量;实验结束后测定体长、脂肪重、血脂(TC、TG、HDL-C和LDL-C)水平等基础指标。结果表明:与基础饮食组相比,高脂饮食组体重、脂肪重、Lee’s指数及血脂水平显著升高(P0.05),说明高脂饮食极易导致肥胖和高血脂;翅果油干预后显著降低小鼠体重、脂肪重、Lee’s指数及血清TC、TG、LDL-C水平(P0.05),其中翅果油中剂量组干预效果最好;且HE染色结果显示,翅果油干预能够抑制高脂饮食引起的脂肪细胞增大,说明翅果油对高脂饮食引起肥胖、高血脂等的发生具有显著抑制作用。(2)动物实验结束后称量肝脏重量,HE染色观察肝脏组织病理学切片、测定肝脂(TG,TC),肝损伤(AST,ALT)和氧化应激(SOD,GSH,MDA)指标。结果表明:与基础饮食组相比,高脂饮食组小鼠肝重、血清ALT和AST水平、肝脏TG、TC水平、肝脏MDA水平显著升高(P0.05),肝脏SOD及GSH水平显著降低(P0.05),且肝脏组织病理学切片显示,高脂饮食组小鼠肝索排列混乱且出现大面积小泡型脂肪变性,说明高脂饮食可诱导非酒精性脂肪肝疾病产生;翅果油干预后,翅果油干预后各项指标均有改善,说明翅果油对非酒精性脂肪肝具有改善作用;相关性结果表明,体重及血清TG与非酒精性脂肪肝相关因子具有显著相关性,说明翅果油可以通过预防肥胖及高血脂改善非酒精性脂肪肝。(3)肝脏脂质合成代谢紊乱是形成非酒精性脂肪肝的主要因素之一。因此,采用实时荧光定量PCR技术,对肝脏脂质合成相关因子(SREBP-1c,PPARγ,ACC和FAS基因)和脂质代谢相关因子(PPARα,CPT-1和ACOX基因)的mRNA表达进行分析,进一步研究翅果油对高脂饮食诱导的非酒精性脂肪肝小鼠改善作用的作用机制。结果表明:翅果油干预使脏脏SREBP-1c,PPARγ和FAS基因mRNA表达下调,使肝脏PPARα和CPT-1基因mRNA表达上调,说明翅果油可通过抑制肝脏脂质合成和加速脂质分解来改善高脂饮食诱导的非酒精性脂肪肝。(4)采用气相色谱/质谱联用法(GC/MS)对肝脏脂肪酸成分及含量进行分析,结果共检测出C14:0,C16:0,C16:1,C16:2,C17:0,C18:0,C18:1,C18:2,ɑ-C18:3,?-C18:3,C20:1,C20:2,C20:3,C20:4和C22:6 15种脂肪酸,OPLS-DA分析结果表明:与高脂饮食组相比,翅果油干预后肝脏C16:2,C18:1,C18:2,ɑ-C18:3,?-C18:3,C20:4和C22:6这7种脂肪酸差异较大;不饱和度分析结果表明,翅果油干预组与高脂饮食组相比,多不饱和脂肪酸尤其是n-3多不饱和脂肪酸差异较大,说明翅果油干预主要影响n-3多不饱和脂肪酸参与肝脏脂肪酸代谢从而改善非酒精性脂肪肝。
【学位单位】：山西大学
【学位级别】：硕士
【学位年份】：2019
【中图分类】：TS201.4
【文章目录】：
中文摘要
ABSTRACT
Chapter 1 Review of the literature
1.1 Overview of Elaeagnus mollis oil
1.1.1 Overview of Elaeagnus mollis Diels
1.1.2 Nutrients of Elaeagnus mollis oil
1.1.3 Health function of Elaeagnus mollis oil
1.2 Overview of non-alcoholic fatty liver disease
1.2.1 Pathogenesis of non-alcoholic fatty liver disease
1.2.2 Therapeutic method of non-alcoholic fatty liver disease
1.3 The significance and main content of the study
1.3.1 The significance of the study
1.3.2 Main content of the study
Chapter 2 Effects of Elaeagnus mollis oil on basic indicators in high-fat diet induced mice
2.1 Introduction
2.2 Materials and methods
2.2.1 Materials
2.2.2 Animal protocol
2.2.3 Collection and preparation of samples from experimental animal
2.2.4 Serum lipids analyses
2.2.5 Histopathology of adipose tissues
2.2.6 Statistical analyses
2.3 Results
2.3.1 Effect of Elaeagnus mollis oil on food intake in high-fat diet induced mice
2.3.2 Effect of Elaeagnus mollis oil on food efficiency ratio in high-fat diet inducedmice
2.3.3 Effect of Elaeagnus mollis oil on energy intake in high-fat diet induced mice
2.3.4 Effect of Elaeagnus mollis oil on body weight in high-fat diet induced mice
2.3.5 Effect of Elaeagnus mollis oil on fat weight and fat coefficient in high-fat dietinduced mice
2.3.6 Effect of Elaeagnus mollis oil on Lee’s index in high-fat diet induced mice
2.3.7 Effect of Elaeagnus mollis oil serum lipids in high-fat diet induced mice
2.3.8 Evaluation of histopathological sections
2.3.9 Correlation analysis
2.4 Discussion
2.5 Conclusions
Chapter 3 Effects of Elaeagnus mollis oil on hepatic lipids accumulation, hepatic injury andhepatic oxidation stress in high-fat diet induced mice
3.1 Introduction
3.2 Materials and methods
3.2.1 Materials
3.2.2 Animal protocol
3.2.3 Collection and preparation of samples from experimental animal
3.2.4 Serum biochemical analyses
3.2.5 Measurement of hepatic profiles
3.2.6 Analyses of antioxidant capacity
3.2.7 Histopathology
3.2.8 Statistical analyses
3.3 Results
3.3.1 Effect of Elaeagnus mollis oil on liver weight and liver coefficient in high-fat diet induced mice
3.3.2 Evaluation of hepatic histopathological sections
3.3.3 Effect of Elaeagnus mollis oil on hepatic lipids in high-fat diet induced mice
3.3.4 Effect of Elaeagnus mollis oil on hepatic injury in high-fat diet induced mice
3.3.5 Effect of Elaeagnus mollis oil on hepatic oxidation stress in high-fat dietinduced mice
3.3.6 Correlation analysis
3.4 Discussion
3.5 Conclusions
Chapter 4 Effects of Elaeagnus mollis oil on hepatic lipids metabolism related mechanisms inhigh-fat diet induced mice
4.1 Introduction
4.2 Materials and methods
4.2.1 Materials
4.2.2 Animal protocol
4.2.3 Collection and preparation of samples from experimental animal
4.2.4 Extraction of m RNA from liver
4.2.5 Reverse transcription
4.2.6 Quantitative real-time PCR （q RT-PCR）
4.2.7 Statistical analyses
4.3 Results
4.3.1 Effect of Elaeagnus mollis oil on SREBP-1c gene expression in high-fat dietinduced mice
4.3.2 Effect of Elaeagnus mollis oil on PPARγ gene expression in high-fat dietinduced mice
4.3.3 Effect of Elaeagnus mollis oil on FAS gene expression in high-fat dietinduced mice
4.3.4 Effect of Elaeagnus mollis oil on ACC gene expression in high-fat dietinduced mice
4.3.5 Effect of Elaeagnus mollis oil on PPARα gene expression in high-fat dietinduced mice
4.3.6 Effect of Elaeagnus mollis oil on CPT-1 gene expression in high-fat dietinduced mice
4.3.7 Effect of Elaeagnus mollis oil on ACOX gene expression in high-fat dietinduced mice
4.3.8 Correlation analysis
4.4 Discussion
4.5 Conclusions
Chapter 5 Effects of Elaeagnus mollis oil on amounts and compositions of fatty acid in liver
5.1 Introduction
5.2 Materials and methods
5.2.1 Materials
5.2.2 Animal protocol
5.2.3 Collection and preparation of samples from experimental animal
5.2.4 Extraction methylation of the fatty acids
5.2.5 Methylation of the fatty acids
5.2.6 Analysis of fatty acids by GC-MS
5.2.7 Statistical analyses
5.3 Results
5.3.1 Effect of Elaeagnus mollis oil on the compositions and contents of fatty acidsin liver
5.3.2 Effect of Elaeagnus mollis oil on the unsaturated degree in liver
5.4 Discussion
5.5 Conclusions
Chapter 6 Conclusion and future work
6.1 Conclusions
6.2 Future work
References
Appendix 1
Appendix 2
攻读学位期间取得的研究成果
致谢
个人简况及联系方式
【相似文献】
本文编号：2850801
【学位单位】：山西大学
【学位级别】：硕士
【学位年份】：2019
【中图分类】：TS201.4
【文章目录】：
中文摘要
ABSTRACT
Chapter 1 Review of the literature
1.1 Overview of Elaeagnus mollis oil
1.1.1 Overview of Elaeagnus mollis Diels
1.1.2 Nutrients of Elaeagnus mollis oil
1.1.3 Health function of Elaeagnus mollis oil
1.2 Overview of non-alcoholic fatty liver disease
1.2.1 Pathogenesis of non-alcoholic fatty liver disease
1.2.2 Therapeutic method of non-alcoholic fatty liver disease
1.3 The significance and main content of the study
1.3.1 The significance of the study
1.3.2 Main content of the study
Chapter 2 Effects of Elaeagnus mollis oil on basic indicators in high-fat diet induced mice
2.1 Introduction
2.2 Materials and methods
2.2.1 Materials
2.2.2 Animal protocol
2.2.3 Collection and preparation of samples from experimental animal
2.2.4 Serum lipids analyses
2.2.5 Histopathology of adipose tissues
2.2.6 Statistical analyses
2.3 Results
2.3.1 Effect of Elaeagnus mollis oil on food intake in high-fat diet induced mice
2.3.2 Effect of Elaeagnus mollis oil on food efficiency ratio in high-fat diet inducedmice
2.3.3 Effect of Elaeagnus mollis oil on energy intake in high-fat diet induced mice
2.3.4 Effect of Elaeagnus mollis oil on body weight in high-fat diet induced mice
2.3.5 Effect of Elaeagnus mollis oil on fat weight and fat coefficient in high-fat dietinduced mice
2.3.6 Effect of Elaeagnus mollis oil on Lee’s index in high-fat diet induced mice
2.3.7 Effect of Elaeagnus mollis oil serum lipids in high-fat diet induced mice
2.3.8 Evaluation of histopathological sections
2.3.9 Correlation analysis
2.4 Discussion
2.5 Conclusions
Chapter 3 Effects of Elaeagnus mollis oil on hepatic lipids accumulation, hepatic injury andhepatic oxidation stress in high-fat diet induced mice
3.1 Introduction
3.2 Materials and methods
3.2.1 Materials
3.2.2 Animal protocol
3.2.3 Collection and preparation of samples from experimental animal
3.2.4 Serum biochemical analyses
3.2.5 Measurement of hepatic profiles
3.2.6 Analyses of antioxidant capacity
3.2.7 Histopathology
3.2.8 Statistical analyses
3.3 Results
3.3.1 Effect of Elaeagnus mollis oil on liver weight and liver coefficient in high-fat diet induced mice
3.3.2 Evaluation of hepatic histopathological sections
3.3.3 Effect of Elaeagnus mollis oil on hepatic lipids in high-fat diet induced mice
3.3.4 Effect of Elaeagnus mollis oil on hepatic injury in high-fat diet induced mice
3.3.5 Effect of Elaeagnus mollis oil on hepatic oxidation stress in high-fat dietinduced mice
3.3.6 Correlation analysis
3.4 Discussion
3.5 Conclusions
Chapter 4 Effects of Elaeagnus mollis oil on hepatic lipids metabolism related mechanisms inhigh-fat diet induced mice
4.1 Introduction
4.2 Materials and methods
4.2.1 Materials
4.2.2 Animal protocol
4.2.3 Collection and preparation of samples from experimental animal
4.2.4 Extraction of m RNA from liver
4.2.5 Reverse transcription
4.2.6 Quantitative real-time PCR （q RT-PCR）
4.2.7 Statistical analyses
4.3 Results
4.3.1 Effect of Elaeagnus mollis oil on SREBP-1c gene expression in high-fat dietinduced mice
4.3.2 Effect of Elaeagnus mollis oil on PPARγ gene expression in high-fat dietinduced mice
4.3.3 Effect of Elaeagnus mollis oil on FAS gene expression in high-fat dietinduced mice
4.3.4 Effect of Elaeagnus mollis oil on ACC gene expression in high-fat dietinduced mice
4.3.5 Effect of Elaeagnus mollis oil on PPARα gene expression in high-fat dietinduced mice
4.3.6 Effect of Elaeagnus mollis oil on CPT-1 gene expression in high-fat dietinduced mice
4.3.7 Effect of Elaeagnus mollis oil on ACOX gene expression in high-fat dietinduced mice
4.3.8 Correlation analysis
4.4 Discussion
4.5 Conclusions
Chapter 5 Effects of Elaeagnus mollis oil on amounts and compositions of fatty acid in liver
5.1 Introduction
5.2 Materials and methods
5.2.1 Materials
5.2.2 Animal protocol
5.2.3 Collection and preparation of samples from experimental animal
5.2.4 Extraction methylation of the fatty acids
5.2.5 Methylation of the fatty acids
5.2.6 Analysis of fatty acids by GC-MS
5.2.7 Statistical analyses
5.3 Results
5.3.1 Effect of Elaeagnus mollis oil on the compositions and contents of fatty acidsin liver
5.3.2 Effect of Elaeagnus mollis oil on the unsaturated degree in liver
5.4 Discussion
5.5 Conclusions
Chapter 6 Conclusion and future work
6.1 Conclusions
6.2 Future work
References
Appendix 1
Appendix 2
攻读学位期间取得的研究成果
致谢
个人简况及联系方式
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