沙棘冻干粉对高脂饮食小鼠的降脂减肥与肠道菌群的调节作用
发布时间：2021-10-11 19:57
如今,由高脂饮食导致的肥胖及其相关的慢性代谢疾病的发生率在全球范围内不断上升,成为全球最为关注的公共健康问题之一。越来越多的证据表明,肥胖及其相关的慢性代谢疾病的发病机制与肠道菌群失调有关。所以,调节肠道菌群被认为是预防和治疗肥胖的潜在靶点。通过使用益生菌或是功能性成分,如多酚物质,植物多糖、黄酮类物质等,来调节肠道微生物并改善肥胖及其并发症是比较有效的方法。沙棘作为药食同源的功能性植物,富含各种营养素和生物活性物质,例如多酚,类黄酮,有机酸,维生素C,多糖,不饱和脂肪酸和人体必需氨基酸等。有研究报道沙棘提取物能够降低血浆胆固醇,增加肠道益生菌,改善脂质代谢酶的活性,增强抗氧化能力以及降低慢性疾病的发病率。沙棘主要以果汁饮料、籽油和提取物等相关加工产品的形式进行消费,对沙棘全果的应用存在一定的局限性。而且考虑到加工过程会造成营养成分的损失,因此,对沙棘全果的相关研究具有很重要的意义。沙棘冻干粉采用低温冻干技术制成,保留了果皮,果肉,果油和籽油等绝大部分的营养成分,具有很强的生物功效。因此,本实验研究了沙棘冻干粉对高脂饮食小鼠的降脂减肥作用和肠道菌群的调节作用。主要内容和结果如下:（1）...
【文章来源】：山西大学山西省
【文章页数】：91 页
【学位级别】：硕士
【文章目录】：
中文摘要
ABSTRACT
Chapter 1 Review of the literature
1.1 Overview of seabuckthorn
1.1.1 Introduction of seabuckthorn
1.1.2 Research progress on seabuckthorn
1.2 Obesity
1.2.1 Obesity definition
1.2.2 The formation and harm of obesity
1.2.3 The Prevention and treatment measures on obesity
1.3 Gut microbiota
1.3.1 Composition and diversity of gut microbiota
1.3.2 Influence factors the diversity on gut microbiota
1.3.3 Research status of the relationship between gut microbiota and obesity
1.3.4 The main metabolite of the gut microbiota-short-chain fatty acids
1.4 Research contents
1.4.1 Research background and significance
1.4.2 Main contents of the research
Chapter 2 Effects of seabuckthorn freeze-dried powder protect against obesity inhigh-fat diet-induced mice
2.1 Introduction
2.2 Materials and Instruments
2.2.1 Materials
2.2.2 Instruments
2.3 Methods
2.3.1 Animals experimental design and dietary treatment
2.3.2 Collection and preparation of samples
2.3.3 Basic indicators measured
2.3.4 Serum and hepatic lipids analysis
2.3.5 Histopathology analysis of liver and adipose tissues
2.3.6 Statistical analysis
2.4 Results
2.4.1 Effects of SFP on body weight and Lee's index in high-fat diet-inducedmice
2.4.2 Effects of SFP on food intake and energy intake in high-fat diet-inducedmice
2.4.3 Effects of SFP on food efficiency ratio and energy efficiency ratio inhigh-fat diet-induced mice
2.4.4 Effects of SFP on the white adipose tissue weight and index in high-fatdiet-induced mice
2.4.5 Effects of SFP on the liver weight and liver index in high-fat diet-inducedmice
2.4.6 Effects of SFP on serum lipids in high-fat diet-induced mice
2.4.7 Effects of SFP on hepatic TC and TG in high-fat diet-induced mice
2.4.8 Pathological alterations of adipose tissue
2.4.9 Pathological alterations of liver
2.5 Chapter Summary
Chapter 3 Effects of seabuckthorn freeze-dried powder on lipids metabolism relatedmechanisms in high-fat diet-induced mice
3.1 Introduction
3.2 Materials and Instrument
3.2.1 Materials
3.2.2 Instruments
3.3 Methods
3.3.1 Animals experimental design and dietary treatment
3.3.2 Collection and preparation of samples
3.3.3 Extraction of the total RNA
3.3.4 Reverse transcription
3.3.5 Real-time quantitative PCR (RT-qPCR)
3.3.6 Statistical analysis
3.4 Results
3.4.1 Effects of SFP on the mRNA expression levels of genes related to fataccumulation,synthesis,and decompostion of epididymal adipose tissue inhigh-fat dict-induced mice
3.4.1.1 Effect of SFP on the PPARγ gene expression in epididymal adiposetissue
3.4.1.2 Effect of SFP on the FAS gene expression in epididymal adiposetissue
3.4.1.3 Effect of SFP on the ACC gene expression in epididymal adiposetissue
3.4.1.4 Effect of SFP on the HSL gene expression in epididymal adiposetissue
3.4.1.5 Effect of SFP on the C/EBP-α gene expression in epididymal adiposetissue
3.4.1.6 Effect of SFP on the SCD1 gene expression in epididymal adiposetissue
3.4.2 Effects of SFP on the mRNA expression levels of genes related to lipidsynthesis and oxidation of hepatic tissue in high-fat diet-induced mice
3.4.2.1 Effect of SFP on the SREBP-1c gene expression in hepatic tissue
3.4.2.2 Effect of SFP on the PPAR-γ gene expression in hepatic tissue
3.4.2.3 Effect of SFP on the FAS gene expression in hepatic tissue
3.4.2.4 Effect of SFP on the ACC gene expression in hepatic tissue
3.4.2.5 Effect of SFP on the PPAR-α gene expression in hepatic tissue
3.4.2.6 Effect of SFP on the CPT-1 gene expression in hepatic tissue
3.4.2.7 Effect of SFP on the ACOX gene expression in hepatic tissue
3.5 Chapter Summary
Chapter 4 Effects of SFP on Gut Microbiota and Short-chain Fatty Acids in high-fatdiet-induced mice
4.1 Introduction
4.2 Materials and Instrument
4.2.1 Materials
4.2.2 Instruments
4.3 Methods
4.3.1 Animals experimental design and dietary treatment
4.3.2 Collection and preparation of fecal samples
4.3.3 Gut microbiota analysis by 16S rRNA gene sequencing
4.3.3.1 Extraction of the fecal total DNA
4.3.3.2 PCR amplification
4.3.3.3 Target region PCR product purification
4.3.3.4 Computer sequencing and sequencing data processing
4.3.4 Gut metabolites analysis by Gas Chromatography(GC)
4.3.5 Statistical analysis
4.4 Results
4.4.1 Effect of SFP on gut microbiota in high-fat diet-induced mice at phylumlevel
4.4.2 Effect of SFP on gut microbiota in high-fat diet-induced mice at familylevel
4.4.3 Effect of SFP on gut microbiota in high-fat diet-induced mice at genus level
4.4.4 Effect of SFP on Gut Metabolites in high-fat diet-induced mice
4.4.5 Correlation analysis
4.5 Chapter Summary
Chapter 5 Conclusion and future work
5.1 Conclusions
5.2 Future work
References
攻读学位期间取得的研究成果
致谢
个人简况及联系方式
【参考文献】：
期刊论文
[1]沙棘葡萄复配果酒的生产工艺优化[J]. 米兰,蒋玉梅,李霁昕,赵毓璇,布鑫荣,毕阳,韩舜愈. 甘肃农业大学学报. 2019(03)
[2]凝固型苦荞酸奶对小鼠肠道菌群的调节作用[J]. 周一鸣,赵燊,冯飞,刘倩,周小理,肖瀛. 食品科学. 2019(13)
[3]有氧运动与复合多糖干预对高脂膳食诱导肥胖大鼠肠道菌群的影响[J]. 沙继斌,张静,隋波,张成岗. 中国运动医学杂志. 2018(04)
[4]沙棘果对高脂膳食大鼠肝脏脂代谢的影响[J]. 杜鹃,宋春梅,李鹏,冯小雨. 中国兽医杂志. 2018(02)
[5]肥胖人群肠道细菌多样性研究[J]. 徐海燕,马慧敏,王彦杰,赵飞燕,刘亚华,海棠,张和平,孙志宏. 中国食品学报. 2018(01)
[6]沙棘的营养价值及其利用[J]. 王志伟. 中国果菜. 2017(11)
[7]新疆沙棘果渣中黄酮的超声提取及成分分析[J]. 康健,王玲,孙文胜,王爱芹,秦亚南,吴桐,张丽霞. 食品安全质量检测学报. 2017(08)
[8]沙棘粉对大鼠肝脏脂质代谢及氧化应激的影响[J]. 杜鹃,郗艳丽,宋春梅. 现代食品科技. 2017(09)
[9]苦荞对高脂膳食诱导小鼠生理及肠道菌群的影响[J]. 周小理,刘泰驿,闫贝贝,王宏,肖瀛,周一鸣. 食品科学. 2018(01)
[10]桑叶提取物对食源性肥胖大鼠的减肥作用及机制研究[J]. 吴雯,梁凯伦,陈波,苏洁,陈素红,吕圭源. 中国中药杂志. 2017(09)
本文编号：3431145
【文章来源】：山西大学山西省
【文章页数】：91 页
【学位级别】：硕士
【文章目录】：
中文摘要
ABSTRACT
Chapter 1 Review of the literature
1.1 Overview of seabuckthorn
1.1.1 Introduction of seabuckthorn
1.1.2 Research progress on seabuckthorn
1.2 Obesity
1.2.1 Obesity definition
1.2.2 The formation and harm of obesity
1.2.3 The Prevention and treatment measures on obesity
1.3 Gut microbiota
1.3.1 Composition and diversity of gut microbiota
1.3.2 Influence factors the diversity on gut microbiota
1.3.3 Research status of the relationship between gut microbiota and obesity
1.3.4 The main metabolite of the gut microbiota-short-chain fatty acids
1.4 Research contents
1.4.1 Research background and significance
1.4.2 Main contents of the research
Chapter 2 Effects of seabuckthorn freeze-dried powder protect against obesity inhigh-fat diet-induced mice
2.1 Introduction
2.2 Materials and Instruments
2.2.1 Materials
2.2.2 Instruments
2.3 Methods
2.3.1 Animals experimental design and dietary treatment
2.3.2 Collection and preparation of samples
2.3.3 Basic indicators measured
2.3.4 Serum and hepatic lipids analysis
2.3.5 Histopathology analysis of liver and adipose tissues
2.3.6 Statistical analysis
2.4 Results
2.4.1 Effects of SFP on body weight and Lee's index in high-fat diet-inducedmice
2.4.2 Effects of SFP on food intake and energy intake in high-fat diet-inducedmice
2.4.3 Effects of SFP on food efficiency ratio and energy efficiency ratio inhigh-fat diet-induced mice
2.4.4 Effects of SFP on the white adipose tissue weight and index in high-fatdiet-induced mice
2.4.5 Effects of SFP on the liver weight and liver index in high-fat diet-inducedmice
2.4.6 Effects of SFP on serum lipids in high-fat diet-induced mice
2.4.7 Effects of SFP on hepatic TC and TG in high-fat diet-induced mice
2.4.8 Pathological alterations of adipose tissue
2.4.9 Pathological alterations of liver
2.5 Chapter Summary
Chapter 3 Effects of seabuckthorn freeze-dried powder on lipids metabolism relatedmechanisms in high-fat diet-induced mice
3.1 Introduction
3.2 Materials and Instrument
3.2.1 Materials
3.2.2 Instruments
3.3 Methods
3.3.1 Animals experimental design and dietary treatment
3.3.2 Collection and preparation of samples
3.3.3 Extraction of the total RNA
3.3.4 Reverse transcription
3.3.5 Real-time quantitative PCR (RT-qPCR)
3.3.6 Statistical analysis
3.4 Results
3.4.1 Effects of SFP on the mRNA expression levels of genes related to fataccumulation,synthesis,and decompostion of epididymal adipose tissue inhigh-fat dict-induced mice
3.4.1.1 Effect of SFP on the PPARγ gene expression in epididymal adiposetissue
3.4.1.2 Effect of SFP on the FAS gene expression in epididymal adiposetissue
3.4.1.3 Effect of SFP on the ACC gene expression in epididymal adiposetissue
3.4.1.4 Effect of SFP on the HSL gene expression in epididymal adiposetissue
3.4.1.5 Effect of SFP on the C/EBP-α gene expression in epididymal adiposetissue
3.4.1.6 Effect of SFP on the SCD1 gene expression in epididymal adiposetissue
3.4.2 Effects of SFP on the mRNA expression levels of genes related to lipidsynthesis and oxidation of hepatic tissue in high-fat diet-induced mice
3.4.2.1 Effect of SFP on the SREBP-1c gene expression in hepatic tissue
3.4.2.2 Effect of SFP on the PPAR-γ gene expression in hepatic tissue
3.4.2.3 Effect of SFP on the FAS gene expression in hepatic tissue
3.4.2.4 Effect of SFP on the ACC gene expression in hepatic tissue
3.4.2.5 Effect of SFP on the PPAR-α gene expression in hepatic tissue
3.4.2.6 Effect of SFP on the CPT-1 gene expression in hepatic tissue
3.4.2.7 Effect of SFP on the ACOX gene expression in hepatic tissue
3.5 Chapter Summary
Chapter 4 Effects of SFP on Gut Microbiota and Short-chain Fatty Acids in high-fatdiet-induced mice
4.1 Introduction
4.2 Materials and Instrument
4.2.1 Materials
4.2.2 Instruments
4.3 Methods
4.3.1 Animals experimental design and dietary treatment
4.3.2 Collection and preparation of fecal samples
4.3.3 Gut microbiota analysis by 16S rRNA gene sequencing
4.3.3.1 Extraction of the fecal total DNA
4.3.3.2 PCR amplification
4.3.3.3 Target region PCR product purification
4.3.3.4 Computer sequencing and sequencing data processing
4.3.4 Gut metabolites analysis by Gas Chromatography(GC)
4.3.5 Statistical analysis
4.4 Results
4.4.1 Effect of SFP on gut microbiota in high-fat diet-induced mice at phylumlevel
4.4.2 Effect of SFP on gut microbiota in high-fat diet-induced mice at familylevel
4.4.3 Effect of SFP on gut microbiota in high-fat diet-induced mice at genus level
4.4.4 Effect of SFP on Gut Metabolites in high-fat diet-induced mice
4.4.5 Correlation analysis
4.5 Chapter Summary
Chapter 5 Conclusion and future work
5.1 Conclusions
5.2 Future work
References
攻读学位期间取得的研究成果
致谢
个人简况及联系方式
【参考文献】：
期刊论文
[1]沙棘葡萄复配果酒的生产工艺优化[J]. 米兰,蒋玉梅,李霁昕,赵毓璇,布鑫荣,毕阳,韩舜愈. 甘肃农业大学学报. 2019(03)
[2]凝固型苦荞酸奶对小鼠肠道菌群的调节作用[J]. 周一鸣,赵燊,冯飞,刘倩,周小理,肖瀛. 食品科学. 2019(13)
[3]有氧运动与复合多糖干预对高脂膳食诱导肥胖大鼠肠道菌群的影响[J]. 沙继斌,张静,隋波,张成岗. 中国运动医学杂志. 2018(04)
[4]沙棘果对高脂膳食大鼠肝脏脂代谢的影响[J]. 杜鹃,宋春梅,李鹏,冯小雨. 中国兽医杂志. 2018(02)
[5]肥胖人群肠道细菌多样性研究[J]. 徐海燕,马慧敏,王彦杰,赵飞燕,刘亚华,海棠,张和平,孙志宏. 中国食品学报. 2018(01)
[6]沙棘的营养价值及其利用[J]. 王志伟. 中国果菜. 2017(11)
[7]新疆沙棘果渣中黄酮的超声提取及成分分析[J]. 康健,王玲,孙文胜,王爱芹,秦亚南,吴桐,张丽霞. 食品安全质量检测学报. 2017(08)
[8]沙棘粉对大鼠肝脏脂质代谢及氧化应激的影响[J]. 杜鹃,郗艳丽,宋春梅. 现代食品科技. 2017(09)
[9]苦荞对高脂膳食诱导小鼠生理及肠道菌群的影响[J]. 周小理,刘泰驿,闫贝贝,王宏,肖瀛,周一鸣. 食品科学. 2018(01)
[10]桑叶提取物对食源性肥胖大鼠的减肥作用及机制研究[J]. 吴雯,梁凯伦,陈波,苏洁,陈素红,吕圭源. 中国中药杂志. 2017(09)
本文编号：3431145
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