基于适配体功能化金属纳米簇新型荧光探针的真菌毒素特异灵敏检测方法研究

发布时间：2022-05-05 19:12

　　食品安全问题是全球首要的公共卫生问题。真菌毒素的存在给食品安全带来了巨大挑战。真菌毒素是一组结构多样的真菌次级代谢产物,可产生刺激机体毒理学作用,痕量的食源性真菌毒素就足以改变机体正常的免疫功能。在农业、药理学、免疫学、环境、医学等领域,与真菌毒素有关的研究已取得了巨大进展,并已建立多种用于测定真菌毒素的常规方法。然而常规和免疫学方法存在明显不足与局限,因此迫切需要开发新型、快速、灵敏和稳定的方法用于检测真菌毒素。近年来,随着适配体功能化的金属纳米簇材料（Metal nanoclusters,MNCs）在生物传感和生物成像领域的应用潜力被不断发掘,发光适配体传感器开始被用于真菌毒素的定量检测。MNC粒径小、合成简便、易于适配体功能化,具备优异的生物相容性和光稳定性,并能有效地将能量转移至其他荧光团和淬灭剂,因此MNCs在荧光传感分析方法中具有广阔的应用前景。在本研究中,合成并表征了几种MNCs,随后将适配体功能化的MNCs与多种纳米材料,如AuNPs,MoS₂和WS₂纳米片复合使用,构建了一系列灵敏、简单、高效和快速的真菌毒素检测方法。首先,... 

【文章页数】：126 页

【学位级别】：博士

【文章目录】：
ACKNOWLEDGEMENTS
DEDICATION
LIST OF ABBREVIATIONS
ABSTRACT
摘要
Chapter.1 General introduction and review of literature
    1.1 Mycotoxin:General introduction
    1.2 Factors affecting mycotoxin production
    1.3 Impact on animal health and productivity
        1.3.1 Mycotoxin-induced toxicities and diseases
    1.4 Important mycotoxins
        1.4.1 AFB1
        1.4.2 ZEN
        1.4.3 T-2 toxin
    1.5 Analytical methods for mycotoxin detection
        1.5.1 Conventional methods
        1.5.2 Immunological methods
        1.5.3 Aptasensor
            1.5.3.1 Element of aptasensor
                1.5.3.1.1 Aptamer
                1.5.3.1.2 Nanomaterials
    1.6 Metal nanoclusters(MNCs)
        1.6.1 Background
        1.6.2 Synthesis of NCs
            1.6.2.1 DNA oligonucleotides
            1.6.2.2 Peptides and proteins
            1.6.2.3 Polymers
            1.6.2.4 Thiols
    1.7 2D materials used in this research
    1.8 Importance of research topic
    1.9 Main objectives of the research
    1.10 References
Chapter2.Silver nanoclusters based FRET aptasensor for sensitive and selective fluorescent detection of T-2 toxin
    Preface
    2.1 Introduction
    2.2 Experimental section
        2.2.1 Chemicals and materials
        2.2.2 Apparatus and characterization
        2.2.3 Synthesis of aptamer functionalized AgNCs
        2.2.4 Fluorescent detection of T-2 toxin
        2.2.5 Sample preparation and measurement
    2.3 Result and discussion
        2.3.1 Principle of AgNCs-MoS_2 nanosheet FRET sensing platform for T-2 toxin
        2.3.2 Characterization of AgNCs and MoS
        2.3.3 Optimization of bioassay
        2.3.4 Feasibility validation of the method
        2.3.5 Analytical performance
        2.3.6 Specificity of the FRET-based apta-assay
        2.3.7 Analytical application
    2.4 Conclusion
    2.5 Key Innovations
    2.6 References
Chapter3.Fabrication of PAA coated green-emitting AuNCs for construction of label-free FRET assembly for specific recognition of T-2 toxin
    Preface
    3.1 Introduction
    3.2 Experimental section
        3.2.1 Chemical
        3.2.2 Instruments
        3.2.3 One-step Synthesis of ATT-AuNCs
        3.2.4 Synthesis of Arg@ATT-AuNCs
        3.2.5 Synthesis of PAA@Arg@ATT-AuNCsNPs
        3.2.6 Preparation of the citrate-protected AuNPs
        3.2.7 General detection procedure of T-2 toxin
        3.2.8 Determination of T-2 in maize sample
    3.3 Results and discussion
        3.3.1 Principle mechanism
        3.3.2 Characterization
        3.3.3 The interaction between PDDA,aptamer,AuNPs,PAA@Arg@ATT-AuNCsNPs and their optimization
        3.3.4 Feasibility affirmation of the bioassay
        3.3.5 Analytical performance
        3.3.6 Specificity
        3.3.7 Analytical Applications
    3.4 Conclusion
    3.5 Key Innovations
    3.6 References
Chapter4:Aptamer induced multi-colored AuNCs-WS_2“Turn on”FRET nano platform for dual-color simultaneous detection of aflatoxin B1 and zearalenone
    Preface
    4.1 Introduction
    4.2 Experimental section
        4.2.1 Chemicals
        4.2.2 Instruments
        4.2.3 Synthesis of blue-emitting(442 nm)and red-emitting AuNCs
        4.2.4 Preparation of AFB1-apt-Lp-AuNCs and ZEN-apt-BSA-AuNCs
        4.2.5 Sample preparation and measurement
        4.2.6 Simultaneous detection of AFB1 and ZEN
    4.3 Result and discussion
        4.3.1 Fluorescent detection method
        4.3.2 Characterization of AuNCs and WS_2
        4.3.3 Optimization
        4.3.4 Feasibility validation of the method
        4.3.5 Analytical performance
        4.3.6 Specificity assay
        4.3.7 Analytical applications
    4.4 Conclusion
    4.5 Key innovations
    4.6 References
Chapter5:General conclusion key innovations and recommendations
    General conclusion
    Key innovations
    Recommendations
    Challenges
List of Publications


【参考文献】：
期刊论文
[1]Copper nanoclusters:Synthesis,characterization and properties[J]. LU YiZhong 1,2 ,WEI WenTao 1,3 &CHEN Wei 1* 1 State key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China; 2 Graduate School of the Chinese Academy of Sciences,Beijing 100049,China; 3 School of Chemistry and Environmental Engineering,Changchun University of Science and Technology,Changchun 130022,China.  Chinese Science Bulletin. 2012(01)



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