两种非洲水稻响应盐胁迫的比较蛋白质组学和磷酸化蛋白质组学分析

发布时间：2021-03-18 11:15

　　水稻是全球超过50%人口的主食（Samiullah Khan,2016）,它被认为是仅次于小麦的全球第二大农产品。水稻具有很高的农业和经济价值,但同时在水稻的种植过程中也面临着生物和非生物等不同类型的胁迫。其中,土壤盐含量在影响全球农业生产的非生物胁迫类型中排名第二。盐胁迫通过干扰植物细胞内的各种生物学过程,导致抑制植物生长从而减少产量。从分子水平研究植物盐胁迫的机理,从而克服盐胁迫影响达到增产的目标是当务之急。本研究旨在于利用对盐胁迫敏感和盐胁迫耐受型水稻品种的比较蛋白质组学分析,从蛋白质及其翻译后修饰水平解析水稻抗盐胁迫的机理。同时也在形态以及生理生化水平上开展了比较研究。主要研究结果显示敏感和耐受品种在盐胁迫下的形态学、生理、生物化学、蛋白质组学和翻译后修饰（磷酸化）发生了显着变化。1)形态学研究揭示了在盐处理1天和3天后,根长、茎长、幼苗长度和侧根数量的统计学发生了显著性变化。2)对盐处理1天和3天的生理生化研究表明,与耐受品种相比,敏感品种的丙二醛（MDA）、电解质渗漏（EL）、钠离子和过氧化氢浓度更高。3)蛋白质组学研究表明,在不同的代谢和调控途径下,其表达模式是相似的、动... 

【文章来源】：中国科学院大学(中国科学院武汉植物园)湖北省

【文章页数】：89 页

【学位级别】：硕士

【文章目录】：
摘要
Abstract
Chapter 1 Background
    1.1 Rice
        1.1.2 Domestication
        1.1.3 Environmental factors affecting rice production
        1.1.4 Rice Food security
    1.2 Introduction to salinity stress
        1.2.1 Salinity
        1.2.2 Impact of salt stress on global agricultural production
        1.2.3 Mechanism of salinity tolerance
        1.2.4 Biochemical and molecular mechanisms of salt tolerance
        1.2.5 Signal transduction
        1.2.6 Crop species with varying degrees of salt tolerance transport in plants
        1.2.7 Input of proteomics （and phospho-proteomics）
    1.3 Objectives
        1.3.1 Overall objective:
        1.3.2 Specific Objectives
Chapter 2 Materials and Methods
    2.1 Rice Culture,Salt Treatment and Harvest
    2.2 Protein extraction
        2.2.1 Protein concentration
        2.2.2 Protein digestion
        2.2.3 IMAC enrichment of phospho-peptides
        2.2.4 Nano-LC-MS/MS analysis
    2.3 MDA determination TBA/TCA plate reader method
    2.4 Electrolyte leakage
    2.5 Potassium and sodium ions determination
    2.6 Determination of Hydrogen peroxide
    2.7 Morphological observation
    2.8 Data analysis
Chapter 3 Results
    3.1 Morphological changes under 300 mM NaCl stress
    3.2 Physiological and biochemical under 300 mM Nacl
        3.2.1 MDA concentration and Hydrogen peroxide under salt stress
        3.2.2 Electrolyte leakage, potassium and sodium ions concentration under saltstress
    3.3 Protein expression under salt stress
        3.3.1 Heat map for the differential expression of proteins
        3.3.2 Metabolism overview under salt stress
        3.3.3 Regulation overview under different pathways
        3.3.4 Protein abundance and function under metabolisms
Chapter 4 Discussion
    4.1 Rice responses at morphological and physiological levels
    4.2 General adaptations of rice to salt stress
    4.3 Salt-responsive signaling pathways in roots
        4.3.1 Calcium regulation, Ca2+ signaling and Ca2+ binding proteins
        4.3.2 Salt sensing and signaling
        4.3.3 G protein mediated signaling
        4.3.4 14-3-3 proteins
        4.3.5 Kinase cascades
    4.4 Phosphorylation
Chapter 5 Conclusion and Prospective
References
Appendices
Acknowledgement
Student Profile
    Academic records
    List of publication during MSc study
    Project affiliation


【参考文献】：
期刊论文
[1]Transport, signaling, and homeostasis of potassium and sodium in plants[J]. Eri Adams,Ryoung Shin.  Journal of Integrative Plant Biology. 2014(03)



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