一株紫色红曲霉液态发酵产红曲色素的特性研究
发布时间：2022-11-09 21:22
红曲霉（Monascus）属微生物菌种可合成有用的次生代谢产物红曲色素（MPs）,它们作为食品色素被广泛应用于食品工业中。此外,MPs在医药、化妆品和纺织行业中也具有良好的市场前景。传统的红曲色素的生产主要采用固态发酵方法,但是固态发酵在规模化放大生产中还存在一定的局限性。相比于固态发酵,采用液态发酵技术生产红曲色素在工业规模上更具有优势和竞争力。本论文以从市售红曲米粉中分离筛选的一株紫色红曲霉（Monascus purpureus）为发酵菌种,研究了不同碳源、氮源等营养物质及其浓度,有机酸代谢调节剂,不同光照条件对该菌株液态发酵产红曲色素的影响,并初步优化了发酵培养基组成和发酵条件,旨在为MPs的液态发酵生产提供参考。主要研究结果如下:（1）影响MPs合成的营养物质种类和浓度的单因素实验结果表明:以葡萄糖为唯一碳源时可获得较高的菌丝体浓度和MPs产量,以甘油为唯一碳源时可促进MPs的合成,但是对菌体的合成影响不显著,葡萄糖和甘油的适宜浓度分别为120 g/L和60 g/L;适宜的氮源为KNO3和蛋白胨,适宜浓度分别为2 g/L和10g/L;KH2
【文章页数】：78 页
【学位级别】：硕士
【文章目录】：
摘要
Abstract
Chapter1 Literature review
1.1 Introduction
1.2 Production of MPs and its influential factors
1.2.1 Production of MPs
1.2.2 Influential factors of pigments production
1.3 Biosynthetic pathway and composition of MPs
1.3.1 Biosynthetic pathway of MPs
1.3.2 Composition of MPs
1.4 Bioactivity of MPs
1.4.1 Antiobesity effect
1.4.2 Anticancer activity
1.4.3 Anti-inflammatory activities
1.4.4 Antidepressant effect
1.4.5 Anti-osteoporosis effect
1.4.6 Anti-diabetic effect
1.5 Application of MPs
1.5.1 Pharmaceutical utilization
1.5.2 Food industry
1.5.3 Uses of MPs in the dye industries
1.6 Problem statement,aims and objectives
1.6.1 Problem statement
1.6.2 Aims and objectives
Chapter2 Materials and methods
2.1 Chemical reagents and experimental instruments
2.1.1 Chemical reagents
2.1.2 Experimental instruments
2.2 Microorganism and seed culture conditions
2.3 Experiment design
2.3.1 Effects of different nutrients on the MPs production by M.purpureus
2.3.2 The orthogonal experimental design
2.3.3 Effect of illumination on the growth and MPs production of M.purpureus
2.3.4 Effect of culture conditions on the growth and MPs production of M.purpureus
2.4 Analytical method
2.4.1 Determination of biomass
2.4.2 Quantification of pigments
2.5 Statistical analysis
Chapter3 Results
3.1 Effects of various carbon sources and concentrations on the MPs production by M.purpureus
3.1.1 Effects of different types of carbon sources
3.1.2 Effects of glucose and glycerol concentrations
3.2 Effects of different nitrogen sources and concentrations on the MPs production byM.purpureus
3.2.1 Effects of different types of nitrogen sources
3.2.2 Effects of peptone and KNO3 concentrations
3.3 Effects of different concentration of inorganic salts and organic acids on the MPsand biomass production by M.purpureus
3.3.1 Effects of inorganic salts
3.3.2 Effects of organic acids
3.4 Optimization of the medium composition for MPs production by OED
3.4.1 Orthogonal experiment result
3.4.2 ANOVA analysis
3.5 Effects of different cultural conditions on the MPs production by M.purpureus
3.5.1 pH value
3.5.2 Volume of medium
3.5.3 Culture temperature
3.5.4 Shaking speed
3.6 Effects of different illumination on the MPs production by M.purpureus
3.6.1 Effects of different light source and intensity on MPs production
3.6.2 Effects of the different light source on the morphology of M.purpureus
3.6.3 The biomass and MPs production under optimized conditions
Chapter4 Discussion
4.1 Effects of various carbon sources and concentrations
4.2 Effects of different nitrogen sources and concentrations
4.3 Effects of different concentration of inorganic salts and organic acids
4.4 Optimization of the medium composition for MPs production by OED
4.5 Effects of different cultural conditions
4.6 Effects of different illumination conditions
4.7 The biomass and MPs production under optimized conditions
Chapter5 Conclusions and prospects
5.1 Conclusions
5.2 Prospects
References
Research achievements during postgraduate study
Acknowledgements
【参考文献】：
期刊论文
[1]铵盐对红曲黄色素、红色素及桔霉素代谢形成的影响(英文)[J]. 周波,朱明军,王菊芳,吴振强,梁世中. 重庆工学院学报(自然科学版). 2009(01)
[2]氮源对红曲霉突变株产黄色素的影响[J]. 周波,浦跃武,朱明军,梁世中. 现代食品科技. 2008(02)
[3]一株产黄色素红曲霉Monascus HB-5的生物学特性研究[J]. 唐秋琳,赵海,王忠彦,戚天胜,刘艳,黄宇峰. 食品科技. 2006(07)
本文编号：3704916
【文章页数】：78 页
【学位级别】：硕士
【文章目录】：
摘要
Abstract
Chapter1 Literature review
1.1 Introduction
1.2 Production of MPs and its influential factors
1.2.1 Production of MPs
1.2.2 Influential factors of pigments production
1.3 Biosynthetic pathway and composition of MPs
1.3.1 Biosynthetic pathway of MPs
1.3.2 Composition of MPs
1.4 Bioactivity of MPs
1.4.1 Antiobesity effect
1.4.2 Anticancer activity
1.4.3 Anti-inflammatory activities
1.4.4 Antidepressant effect
1.4.5 Anti-osteoporosis effect
1.4.6 Anti-diabetic effect
1.5 Application of MPs
1.5.1 Pharmaceutical utilization
1.5.2 Food industry
1.5.3 Uses of MPs in the dye industries
1.6 Problem statement,aims and objectives
1.6.1 Problem statement
1.6.2 Aims and objectives
Chapter2 Materials and methods
2.1 Chemical reagents and experimental instruments
2.1.1 Chemical reagents
2.1.2 Experimental instruments
2.2 Microorganism and seed culture conditions
2.3 Experiment design
2.3.1 Effects of different nutrients on the MPs production by M.purpureus
2.3.2 The orthogonal experimental design
2.3.3 Effect of illumination on the growth and MPs production of M.purpureus
2.3.4 Effect of culture conditions on the growth and MPs production of M.purpureus
2.4 Analytical method
2.4.1 Determination of biomass
2.4.2 Quantification of pigments
2.5 Statistical analysis
Chapter3 Results
3.1 Effects of various carbon sources and concentrations on the MPs production by M.purpureus
3.1.1 Effects of different types of carbon sources
3.1.2 Effects of glucose and glycerol concentrations
3.2 Effects of different nitrogen sources and concentrations on the MPs production byM.purpureus
3.2.1 Effects of different types of nitrogen sources
3.2.2 Effects of peptone and KNO3 concentrations
3.3 Effects of different concentration of inorganic salts and organic acids on the MPsand biomass production by M.purpureus
3.3.1 Effects of inorganic salts
3.3.2 Effects of organic acids
3.4 Optimization of the medium composition for MPs production by OED
3.4.1 Orthogonal experiment result
3.4.2 ANOVA analysis
3.5 Effects of different cultural conditions on the MPs production by M.purpureus
3.5.1 pH value
3.5.2 Volume of medium
3.5.3 Culture temperature
3.5.4 Shaking speed
3.6 Effects of different illumination on the MPs production by M.purpureus
3.6.1 Effects of different light source and intensity on MPs production
3.6.2 Effects of the different light source on the morphology of M.purpureus
3.6.3 The biomass and MPs production under optimized conditions
Chapter4 Discussion
4.1 Effects of various carbon sources and concentrations
4.2 Effects of different nitrogen sources and concentrations
4.3 Effects of different concentration of inorganic salts and organic acids
4.4 Optimization of the medium composition for MPs production by OED
4.5 Effects of different cultural conditions
4.6 Effects of different illumination conditions
4.7 The biomass and MPs production under optimized conditions
Chapter5 Conclusions and prospects
5.1 Conclusions
5.2 Prospects
References
Research achievements during postgraduate study
Acknowledgements
【参考文献】：
期刊论文
[1]铵盐对红曲黄色素、红色素及桔霉素代谢形成的影响(英文)[J]. 周波,朱明军,王菊芳,吴振强,梁世中. 重庆工学院学报(自然科学版). 2009(01)
[2]氮源对红曲霉突变株产黄色素的影响[J]. 周波,浦跃武,朱明军,梁世中. 现代食品科技. 2008(02)
[3]一株产黄色素红曲霉Monascus HB-5的生物学特性研究[J]. 唐秋琳,赵海,王忠彦,戚天胜,刘艳,黄宇峰. 食品科技. 2006(07)
本文编号：3704916
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