PCR-RFLP在mtDNA多态性分析中的应用

发布时间：2018-01-07 13:36

本文关键词：PCR-RFLP在mtDNA多态性分析中的应用　出处：《中国法医学杂志》2011年01期 　论文类型：期刊论文

【摘要】：目的探讨PCR-RFLP法在mtDNA多态性分析中的应用价值。方法应用限制酶RsaI和MnlI消化mtDNA D-LOOP区(HVI16106～16297)PCR扩增产物,聚丙烯凝胶电泳进行RFLP分型,对不同PCR-RFLP分型扩增产物进行测序,并对150例辽宁汉族随机个体及30例真三联家系血样进行检验。结果在150例随机个体中,RsaI和MnlI酶切分别发现3和8种表型,DNA测序结果和PAGE分型结果一致,GD值分别为0.107,0.670。联合两种酶切,发现12种表型,GD值为0.708。在30个家系中,母子的RFLP带型完全相同。结论 PCR-RFLP法适合在基层实验室mtDNA分析中应用。
[Abstract]:Objective to evaluate the value of PCR-RFLP method in mtDNA polymorphism analysis. Methods mtDNA D-LOOP region was digested by restriction enzyme RsaI and MnlI. HVI16106~16297)PCR amplification products. Polypropylene gel electrophoresis was used for RFLP typing and different PCR-RFLP typing products were sequenced. The blood samples of 150 Han nationality random individuals and 30 true triple families were tested. Results three and eight phenotypes were found in 150 random individuals by RsaI and MnlI digestion, respectively. The GD values of DNA sequencing and PAGE typing were 0.107 and 0.670, respectively. Combined with two enzymes, the GD values of 12 phenotypes were 0.708.In 30 families, the GD values of 12 phenotypes were found to be 0.708. Conclusion the PCR-RFLP method is suitable for mtDNA analysis in basic laboratory.
【作者单位】：南通大学医学院法医学教研室;南通大学附属医院普外科;川北医学院法医系;中国医科大学法医学院法医血清教研室;
【分类号】：D919
【正文快照】： 人类线粒体DNA(m itochondrial DNA,m tDNA)是细胞核外唯一的遗传物质,因其拷贝数多、多态性高、呈母系遗传等特点而应用于微量、降解检材的DNA检验。本研究采用PCR-RFLP技术对m tDNA在法医实践中的应用价值进行探讨。1材料与方法1.1样本150例辽宁汉族健康无关个体及30例家系

【参考文献】